Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
100 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
100 µg Transfection-Ready DNA Clear filter

27,469 results

Showing: 5761 - 5780 of 27469 results

  3. pdCas9-DNMT3A-PuroR_hNT

    Plasmid
    #71830
    Purpose
    Expression of dCas9-DNMT3A fusion with T2A-PuroR and non-targeting control sgRNA for use in human cells
    Depositor
    Vlatka Zoldos
    Publication
    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Non-targeting sgRNA human (DNMT3A Synthetic, Human, S. pyogenes)
    Use
    CRISPR
    Tags
    3xFLAG, SV40 NLS, and T2A-PuroR
    Expression
    Mammalian
    Mutation
    D10A and H840A in S.pyogenes Cas9
    Promoter
    U6
    Available since
    March 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    71830-plasmid-map-sequence-id-131025

    Enlarge

  4. BPK2797

    Plasmid
    #72251
    Purpose
    Human expression plasmid for SpCas9-VRQR variant: CMV-T7-humanSpCas9-VRQR(D1135V, G1218R, R1335Q, T1337R)-NLS-3xFLAG
    Depositor
    Keith Joung
    Publication
    Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 VRQR(D1135V/G1218R/R1335Q/T1337R)-NLS-3xFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335Q, and T1337R
    Promoter
    CMV
    Available since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    72251-plasmid-map-sequence-id-131872

    Enlarge

  8. pGL3 Zscan4 promoter

    Plasmid
    #69249
    Purpose
    Luciferase reporter for Dux4 activation of Zscan4 transcription
    Depositor
    Stephen Tapscott
    Publication
    Geng et al Dev Cell. 2012 Jan 17;22(1):38-51. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Zscan4 (ZSCAN4 Human)
    Use
    Luciferase
    Mutation
    The two single nucleotide mismatches found betwee…
    Promoter
    Zscan4
    Available since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    69249-plasmid-map-sequence-id-123962

    Enlarge

  9. GIPR-Tango

    Plasmid
    #66294
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GIPR (GIPR Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  10. APJ-Tango

    Plasmid
    #66224
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Publication
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    APJ (APLNR Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  11. GFP-TRIM66

    Plasmid
    #65400
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Publication
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TRIM66 (TRIM66 Human)
    Tags
    GFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  12. TPST2-EGFP

    Plasmid
    #66618
    Purpose
    Encodes GFP fusion of TPST2 trans Golgi marker (Tyrosyl protein sulfotransferase 2)
    Depositor
    David Stephens
    Publication
    Spooner et al Biochem J. 2008 Sep 15;414(3):471-84. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TPST2 (TPST2 Human)
    Expression
    Mammalian
    Mutation
    Insert contains T370A relative to NM_001008566.1
    Available since
    July 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  15. sfGFP-TFR-20

    Plasmid
    #56488
    Purpose
    Localization: Transferrin Receptor, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TFR (TFRC Human)
    Tags
    sfGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    56488-plasmid-map-sequence-id-271349

    Enlarge

  16. pDest eGFP CUGBP1

    Plasmid
    #61276
    Purpose
    Mammalian expression of EGFP-tagged human CUGBP1
    Depositor
    Nicolas Charlet-Berguerand
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CUG-BP (CELF1 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    61276-plasmid-map-sequence-id-99587

    Enlarge

  17. pEGFP-N3 alpha-SSeCKS

    Plasmid
    #61294
    Purpose
    Mammalian expression of GFP-alpha-SSeCKS
    Depositor
    Irwin Gelman
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    alpha SSeCKS (Akap12 Rat)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Dec. 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  18. pBI-MCS-EYFP

    Plasmid
    #58855
    Purpose
    The tet-responsive reporter pBI-MCS-EGFP (Addgene plasmid 16542) was modified to express YFP instead of GFP
    Depositor
    Joshua Leonard
    Publication
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    enhanced yellow fluorescent protein
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    58855-plasmid-map-sequence-id-98989

    Enlarge

  19. mCherry-Alpha-Actin-12

    Plasmid
    #54971
    Purpose
    Localization: Actin, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Alpha-Actin (ACTA2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    54971-plasmid-map-sequence-id-239313

    Enlarge

Showing: 5761 - 5780 of 27469 results