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27,466 results

Showing: 11961 - 11980 of 27466 results

  1. Level 1 P4 TaU6 guide acceptor

    Plasmid
    #165600
    Purpose
    GoldenGate (MoClo) Level 1 Position 4 TaU6 guide acceptor plasmid
    Depositor
    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
    Publication
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TaU6 promoter::LacZ::sgRNA scaffold
    Use
    CRISPR and Synthetic Biology; Sgrna acceptor with…
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165600-plasmid-map-sequence-id-331273

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  2. Level 1 P3 TaU6 guide acceptor

    Plasmid
    #165599
    Purpose
    GoldenGate (MoClo) Level 1 Position 3 TaU6 guide acceptor plasmid
    Depositor
    Wendy Harwood, John Innes Centre - Wheat Transformation and Genome Editing
    Publication
    Smedley et al Curr Protoc. 2021 Mar;1(3):e65. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TaU6 promoter::LacZ::sgRNA scaffold
    Use
    CRISPR and Synthetic Biology; Sgrna acceptor with…
    Available since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    165599-plasmid-map-sequence-id-331270

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  8. pmiCas9-msa

    Plasmid
    #164833
    Purpose
    miCas9-msa fusion protein expressing plasmid
    Depositor
    Eugene Chen
    Publication
    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miCas9-msa
    Use
    CRISPR
    Tags
    FLAG and NLS
    Expression
    Mammalian
    Mutation
    contains mSA sequence range is 5106-5447 and Brex…
    Promoter
    CMV
    Available since
    April 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    164833-plasmid-map-sequence-id-329431

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  10. POLArISact

    Plasmid
    #164971
    Purpose
    Expresses POLArISact, an orientation probe that specifically binds to F-actin, in mammalian cells
    Depositor
    Sumio Terada
    Publication
    Sugizaki et al Proc Natl Acad Sci U S A. 2021 Mar 16;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    POLArISact
    Tags
    HA
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    164971-plasmid-map-sequence-id-326985

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  13. pCMV-T7-AsCas12a-P2A-EGFP (RTW2861)

    Plasmid
    #160140
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized AsCas12a with a c-terminal NLS(nucleoplasmin), 3x HA tag, and P2A-EGFP
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized AsCas12a with NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(nucleoplasmin)-3xHA-P2A-EGFP
    Expression
    Mammalian
    Promoter
    CMV and T7
    Available since
    Feb. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    160140-plasmid-map-sequence-id-317331

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  16. Nup133_mEGFP(-8a)

    Plasmid
    #163417
    Purpose
    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)
    Depositor
    Sanford Simon
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nup133 (NUP133 Human)
    Tags
    mEGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    163417-plasmid-map-sequence-id-321553

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  19. pCMV_eA3A-NG-nCas9

    Plasmid
    #163536
    Purpose
    Mammalian CG-to-GC base editing
    Depositor
    David Liu
    Publication
    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    eA3A-NG-nCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…
    Available since
    Dec. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    163536-plasmid-map-sequence-id-320176

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Showing: 11961 - 11980 of 27466 results