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27,462 results

Showing: 8341 - 8360 of 27462 results

  1. dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP

    Plasmid
    #154139
    Purpose
    Expresses the dCas9-DNMT3a-DNMT3l-3xFLAG-tagBFP fusion protein for targeted DNA methylation.
    Depositor
    Albert Jeltsch
    Publication
    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9, DNMT3a (catalytic domain), DNMT3l (C-terminal part), tagBFP (DNMT3A, DNMT3L Human, Streptococcus pyogenes)
    Tags
    3x FLAG and tagBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    154139-plasmid-map-sequence-id-345683

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  2. 10TALE_Pmin_fLuc

    Plasmid
    #160663
    Purpose
    Expression of the firefly luciferase reporter upon binding of a transcriptional activator to target sites. The plasmid contains 10 copies of target sites for the TALEN1257 DNA-binding domain
    Depositor
    Roman Jerala
    Publication
    Mesko et al ACS Synth Biol. 2020 Aug 21;9(8):2055-20
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    10TALE_Pmin_fLuc
    Use
    Luciferase
    Expression
    Mammalian
    Promoter
    minimal
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160663-plasmid-map-sequence-id-345378

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  4. DDX41-GFP

    Plasmid
    #175494
    Purpose
    Total RNA was isolated from TF-1 cells and converted to cDNA using SuperScript III (Invitrogen). The full DDX41 coding sequence was cloned into pcDNA3.1 (Invitrogen) with an EGFP c-terminal insert.
    Depositor
    Evan Braunstein
    Publication
    Li et al Haematologica. 2016 Jun;101(6):e228-31.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDX41 (DDX41 Human)
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Oct. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    175494-plasmid-map-sequence-id-345045

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  5. pSF4 TetCMV ATF4(5'UTR)-SunTag-Renilla-FKBP-Stop-24xMS2v5-CTE-polyA

    Plasmid
    #164615
    Purpose
    Expresses ATF4(5'UTR)-SunTag-Renilla mRNA reporter under tetracycline-inducible promoter. SunTag and Renilla luciferase are in frame with the main start codon of ATF4.
    Depositor
    Jeffrey Chao
    Publication
    Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Renilla luciferase
    Tags
    24xMS2v5 (3'UTR) and SunTag
    Expression
    Bacterial and Mammalian
    Promoter
    TetCMV
    Available since
    Sept. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    164615-plasmid-map-sequence-id-325006

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  6. pX330 _CRISPIE donor B8

    Plasmid
    #172849
    Purpose
    DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B8 (Zhong et al, eLife 2021), mEGFP translational phase (1-1), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DRS-2 sgRNA and CRISPIE designer exon (phase 1-1) encoding mEGFP
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    172849-plasmid-map-sequence-id-340928

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  9. pcDNA3.1-GGamma5-GFP2

    Plasmid
    #166777
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGamma5-GFP2 (GNG5 Human, Synthetic)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    166777-plasmid-map-sequence-id-408989

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  10. pcDNA3.1-GGamma3-GFP2

    Plasmid
    #166775
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGamma3-GFP2 (GNG3 Human, Synthetic)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    May 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    166775-plasmid-map-sequence-id-330996

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  11. GLuc-PM-Venus

    Plasmid
    #164785
    Purpose
    Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein
    Depositor
    Laszlo Hunyady
    Publication
    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Gaussia princeps)
    Tags
    GLuc and mVenus
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    May 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    164785-plasmid-map-sequence-id-326677

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  14. pCDNA5_FRT_TO_TurboID-mCherry-Cry2

    Plasmid
    #166504
    Purpose
    Expression of your protein of interest fused with TurboID and mcherry-CRY2 for optogenetic activation, Flp-In T-Rex system. Search for client proteins within light-induced biomolecular condensates.
    Depositor
    Angelos Constantinou
    Publication
    Frattini et al Mol Cell. 2021 Jan 16. pii: S1097-2765(2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    None
    Tags
    TurboID and mCherry-CRY2
    Expression
    Mammalian
    Available since
    March 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    166504-plasmid-map-sequence-id-328214

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Showing: 8341 - 8360 of 27462 results