Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

27,462 results

Showing: 9101 - 9120 of 27462 results

  1. mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax

    Plasmid
    #173870
    Purpose
    Mammalian expression of mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax (opto-RhoA)
    Depositor
    Takao Nakata
    Publication
    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-NES-SspB-LARG-DH-P2A-iLIDcaax (ARHGEF12 Avena sativa (oat), Human)
    Tags
    C-terminal CAAX-box and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    173870-plasmid-map-sequence-id-341719

    Enlarge

  3. PEA1-Nuclease-Puro

    Plasmid
    #171992
    Purpose
    Delivers all prime editing nuclease components in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CbH-Cas9-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    Aug. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    171992-plasmid-map-sequence-id-338139

    Enlarge

  4. DENV4 Rluc Reporter GND mutant Replicon

    Plasmid
    #118586
    Purpose
    For use as a positive control for translation of the Rluc gene in the transfected cells and negative control for replication.
    Depositor
    Radhakrishnan Padmanabhan
    Publication
    Alcaraz-Estrada et al J Gen Virol. 2010 Nov;91(Pt 11):2713-8.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DENV4 Renilla luciferase gene (Rluc) reporter replicon containing replication-defective GND mutation in the NS5 polymerase gene.
    Use
    Yeast-e. col shuttle vector prs424
    Mutation
    The conserved GDD in the NS5 polymerase gene muta…
    Available since
    Aug. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    118586-plasmid-map-sequence-id-227283

    Enlarge

  6. pCAG-spCas9-N57

    Plasmid
    #169920
    Purpose
    Expression of SpCas9-N57 fusion protein to enhace site-specific DNA integration.
    Depositor
    Zhili Rong
    Publication
    Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-N57
    Use
    CRISPR
    Mutation
    N57 is the PAI domain of SB100X
    Promoter
    CAG
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    169920-plasmid-map-sequence-id-336052

    Enlarge

  7. pCAG-spCas9-SB

    Plasmid
    #169919
    Purpose
    Expression of SpCas9-SB100X transposase fusion protein to enhace site-specific DNA integration.
    Depositor
    Zhili Rong
    Publication
    Ma et al Nucleic Acids Res. 2020 Oct 9;48(18):105
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-SB100X
    Use
    CRISPR
    Promoter
    CAG
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    169919-plasmid-map-sequence-id-336025

    Enlarge

  9. pcDNA3.1-GGamma10-GFP2

    Plasmid
    #166779
    Purpose
    Encodes a G gamma subunit containing GFP2 as an "non-optimal" component of a BRET2 biosensor for studying heterotrimeric G protein dissociation
    Depositor
    Bryan Roth
    Publication
    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GGamma10-GFP2 (GNG10 Synthetic, Human)
    Expression
    Mammalian
    Mutation
    Contains an N-terminal GFP2 and GSAG linker
    Promoter
    CMV
    Available since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    166779-plasmid-map-sequence-id-331002

    Enlarge

  14. pPGC1a_Distal Promoter-FLuc2P

    Plasmid
    #154260
    Purpose
    Destabilized firefly luciferase reporter containing the upstream Human PGC-1alpha Distal Promoter from -500 to +52.
    Depositor
    Antonio Amelio
    Publication
    Musicant et al Cell Rep. 2021 Feb 23;34(8):108768. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PGC-1a Distal Promoter (PPARGC1A Human)
    Expression
    Mammalian
    Promoter
    Human PGC-1alpha Distal Promoter
    Available since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    154260-plasmid-map-sequence-id-306859

    Enlarge

  15. pCS2+ GCamp6-3NLS

    Plasmid
    #165401
    Purpose
    Expresses a nuclear localized genetically encoded calcium reporter.
    Depositor
    Philipp Niethammer
    Publication
    Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Nuclear Localization Signal
    Use
    Avian, xenopus, zebrafish
    Tags
    GCamp6
    Expression
    Mammalian
    Promoter
    SP6
    Available since
    March 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    165401-plasmid-map-sequence-id-325983

    Enlarge

  19. GEF-H1 FLARE191.pa

    Plasmid
    #134931
    Purpose
    GEF-H1 biosensor that has GEF activity.
    Depositor
    Klaus Hahn
    Publication
    Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)
    Tags
    None.
    Expression
    Bacterial and Mammalian
    Promoter
    CMV and T7
    Available since
    Dec. 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    134931-plasmid-map-sequence-id-319656

    Enlarge

Showing: 9101 - 9120 of 27462 results