Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

Applied Filters

Clear All Filters
50 µg Transfection-Ready DNA Clear filter

27,462 results

Showing: 10181 - 10200 of 27462 results

  4. pEGFP-NMHC-IIA-3xA

    Plasmid
    #101041
    Purpose
    Expresses GFP-MYH9 construct (human myosin IIA) carrying S1943A mutation and S1916A, S1915A mutation, driven by CMV promoter. Thus inhibits CKII and PKC phosphorylation of the tail.
    Depositor
    Tom Egelhoff
    Publication
    Rai et al J Biol Chem. 2017 Feb 24;292(8):3099-311
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Non-muscle myosin IIA heavy chain (MYH9 Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    S1915A, S1916A, S1943A
    Promoter
    CMV
    Available since
    Nov. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    101041-plasmid-map-sequence-id-343684

    Enlarge

  6. AICSDP-27:SLC25A17-mEGFP

    Plasmid
    #101784
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human SLC25A17
    Depositor
    Allen Institute for Cell Science
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SLC25A17 Homology Arms with linker-mEGFP (SLC25A17 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    1 Article
    101784-plasmid-map-sequence-id-197677

    Enlarge

  7. pCAGGS-hPhyBY276H-mCherry-HRasCT

    Plasmid
    #100540
    Purpose
    Expresses Phytochrome B-Y276H (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells
    Depositor
    Kazuhiro Aoki
    Publication
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Phytochrome B (1-908 aa, Y276H mutation) (PHYB Mustard Weed)
    Tags
    mCherry fused with HRas C-terminus for plasma mem…
    Expression
    Mammalian
    Mutation
    Human codon optimized PhyB (1-908 aa), Y276H muta…
    Promoter
    CAG promoter
    Available since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    100540-plasmid-map-sequence-id-197261

    Enlarge

  8. p458 VRER

    Plasmid
    #101728
    Purpose
    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 VRER
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    VRER (D1135V, G1218R, R1335E, and T1337R mutation…
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    101728-plasmid-map-sequence-id-196591

    Enlarge

  9. px458 EQR

    Plasmid
    #101731
    Purpose
    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs
    Depositor
    Andrew Holland
    Publication
    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9 EQR
    Tags
    3xFLAG-NLS and NLS
    Expression
    Mammalian
    Mutation
    EQR (D1135E, R1335Q, and T1337R mutations in Cas9)
    Available since
    Oct. 17, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    101731-plasmid-map-sequence-id-196590

    Enlarge

  11. pOTTC591 - pCMV Mem-iRFP

    Plasmid
    #59134
    Purpose
    A plasmid that express membrane-targeted iRFP under the CMV promoter.
    Depositor
    Brandon Harvey
    Publication
    Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Membrane-localized Infrared Fluorescent Protein
    Tags
    Palmitylation signal
    Expression
    Mammalian
    Promoter
    CMV-IE
    Available since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    59134-plasmid-map-sequence-id-194618

    Enlarge

  12. CD8-pTagRFP657-LOVpep (T406A, T407A, I532A)

    Plasmid
    #100742
    Purpose
    To express a plasma membrane "anchor" in mammalian cells to hot-wire endocytosis
    Depositor
    Stephen Royle
    Publication
    Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CD8A (CD8A Human)
    Tags
    LOVpep and tagRFP657
    Expression
    Mammalian
    Mutation
    Truncated
    Promoter
    CMV
    Available since
    Sept. 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    100742-plasmid-map-sequence-id-194015

    Enlarge

  13. pIRES-hrGFP II-mTET1_CD

    Plasmid
    #83571
    Purpose
    Expresses a mutant catalytic domain of TET1 in mammalian cells
    Depositor
    Jean-Pierre Issa
    Publication
    Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tet Methylcytosine Dioxygenase 1 (TET1 Human)
    Tags
    3X FLAG tag and hr GFP II
    Expression
    Mammalian
    Mutation
    TET1 amino acids 1-1417 deleted, catalytic domain…
    Promoter
    CMV
    Available since
    Sept. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    83571-plasmid-map-sequence-id-191052

    Enlarge

  17. WT-Pan

    Plasmid
    #99638
    Purpose
    EGFP-mCherry control reporter for methionine misincorporation
    Depositor
    Tao Pan
    Publication
    Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry
    Tags
    EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    99638-plasmid-map-sequence-id-190530

    Enlarge

  19. pLinker-6xHA-HXGPRT-LoxP

    Plasmid
    #86552
    Purpose
    The plasmid is used for endogenous tagging using CRISPR technology in Toxoplasma
    Depositor
    David Sibley
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Linker-6xHA-HGPRT-LoxP
    Use
    Expression in toxoplasma gondii
    Available since
    Aug. 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    86552-plasmid-map-sequence-id-190138

    Enlarge

Showing: 10181 - 10200 of 27462 results