27,462 results
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Plasmid#111824PurposeKnockout eGFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA targeting eGFP 477-497
UseCRISPRTagsmCherryExpressionBacterial and MammalianPromoterU6Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-NES-ZapCV2 (cpV143)
Plasmid#112060PurposeGenetically-encoded Cyan-Yellow Zinc FRET sensor. Localizes to the cytosol. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNES-ZapCV2
TagsNuclear Export Signal (NES) derived from HIV-1ExpressionMammalianMutationECFP gene is truncated 36 bp at 3' end. Venu…PromoterCMVAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRPaCas9
Plasmid#111819PurposeExpresses Cas9 in T. brucei (2T1) cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9
UseCRISPRAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
tCRMod-GGSGGS7
Plasmid#111761PurposeExpresses soluble Clover-mRuby2 tension sensor module with 7-repeat GGSGGS extensible domainDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertClover-GGSGGS7-mRuby2 fusion
ExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCS6-StaPLd-Casp9
Plasmid#111511PurposeExpresses a tandem caspase-9 dimer, governed by a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStaPLd-Casp9
ExpressionMammalianMutationThe HCV NS3 protease carries V36M, T54A, and S122…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry Codon 59 sgRNA
Plasmid#109431PurposeMLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry codon 59 gRNA
UseCRISPRExpressionMammalianPromoterhU6Available sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-N1-EXO1b-D173A
Plasmid#111622PurposeExpression of human catalitic dead Exonuclease 1 (EXO1) mCherry-tagged in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertExonuclease 1b D173A (EXO1 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mVenusC-Cavin1
Plasmid#108888PurposeExpression of Cavin1 fused to the C-terminus of mVenus for bimolecular fluorescence complementationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCavin1 (altered codon usage) (CAVIN1 Human, Synthetic)
TagsmVenus156-239ExpressionMammalianMutationaltered codon usageAvailable sinceJune 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChR2-mRuby2-ST
Plasmid#109125PurposeCation channelrhodopsin ChR2 fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertChR2-mRuby2-ST
ExpressionMammalianPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mVenusN-Cavin1
Plasmid#108887PurposeExpression of Cavin1 fused to the N-terminus of mVenus for bimolecular fluorescence complementationDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcaveolae associated protein 1 (CAVIN1 Human, Synthetic)
TagsmVenus1-155ExpressionMammalianMutationaltered codon usageAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV iGluu
Plasmid#105832PurposeFast indicator for extracellular glutamate imagingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiGluSnFR S72T variant
TagsMyc and PDGFRExpressionMammalianMutationChanged Ser 72 to ThrPromoterCMVAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
Human USP30 (1-517, C77A, MG-38-16)
Plasmid#110749PurposeTransient transfection of human USP30 C77ADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertUSP30 (USP30 Human)
ExpressionMammalianMutationC77APromoterCMVAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAC_N_LMNA
Plasmid#111688PurposeMAC-tagged gene expressionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLMNA (LMNA Human)
ExpressionMammalianAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
cmamxA2-GFP
Plasmid#107197PurposeExpresses human annexin A2-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deletedDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertcmannexin A2 (ANXA2 Human)
TagsGreen Fluorescent ProteinExpressionMammalianMutationall type II Ca2+ binding sites deleted ( D161A, E…PromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEM705 Gs12
Plasmid#109357PurposeGsX chimera with Gα12 C-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGs12 chimera
TagsNoneExpressionMammalianPromoterCAGAvailable sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmiRFP-N1
Plasmid#108409PurposeMonomeric near-infrared fluorescent protein miRFP in mammalian expression plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmiRFP
ExpressionMammalianPromoterCMVAvailable sinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-FKBP-SAC1deltaTMD
Plasmid#108132PurposeRecruitable cytosolic SAC1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) (SACM1L Human)
ExpressionMammalianMutationSACM1L(1-521)PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGL3 2 kb prom. and exon 1 fragment CD274
Plasmid#107007PurposeModified Luciferase expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD274 (CD274 Human)
UseLuciferaseExpressionMammalianAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-HER3-DMVF-181-R
Plasmid#87621PurposeC-terminal Dronpa fragment fused to the C-terminus of full-length HER3; for BiFC.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHER3-DMVF181-C (ERBB3 Human, Synthetic)
ExpressionMammalianPromoterCMVAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
aL-EGFP
Plasmid#103879Purposefull-length human aL with EGFP at betapropellerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertfull-length human aL with EGFP in betapropeller (ITGAL Human)
Tagstension sensor moduleAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only