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p264 pSP72 hAR-LBD(LNCaP)

Plasmid

#12495

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res. 2003 Aug 1. 31(15):e8

Insert

AR LBD (AR Human)

Expression

Bacterial

Mutation

Thr 878 Ala in AR-LBD

Available Since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only
LB335 (Bcl-2 from ATG to -1281)

Plasmid

#15382

Depositor

Linda Boxer

Article

Heckman et al J Biol Chem. 2000 Mar 3. 275(9):6499-508

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from ATG to -1281 with P2

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
4MVT

Plasmid

#60101

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

PIAS3:YTC008-B10:C225910 (PIAS3 Human)

Tags

6xHis

Expression

Bacterial

Mutation

See comments

Promoter

T7

Available Since

Oct. 22, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pET26b(+)-ATI_QconCAT_1

Plasmid

#163957

Purpose

Exression of ATI QconCAT protein in E. coli expression strains (BL21). Encodes a concatemer of tryptic peptides from wheat amylase/trypsin inhibitors. Used as standard for LC-MS-based quantification.

Depositor

Stefan Tenzer

Article

Sielaff et al J Proteome Res. 2021 Jan 28. doi: 10.102

Insert

ATI QconCAT

Tags

polyhistidine tag

Expression

Bacterial

Promoter

T7 promoter

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-Ubl

Plasmid

#78712

Purpose

Expresses Sacsin Ubl- domain (2-85) in fusion with GST (bacterial)

Depositor

Kalle Gehring

Article

Menade et al J Biol Chem. 2018 Aug 17;293(33):12832-1

Insert

Human Sacsin 2-85

Tags

GST-Tag

Expression

Bacterial

Available Since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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pCoofy33

Plasmid

#44001

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His10 and OneStrep

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp7

Plasmid

#156476

Purpose

Bacterial expression of Sars-CoV2 Nsp7 protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pFLAG-CrmA

Plasmid

#11831

Depositor

Guy Salvesen

Article

Muzio et al J Biol Chem. 1997 Jan 31. 272(5):2952-6.

Insert

CrmA (CrmA Cowpox virus)

Expression

Bacterial

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
phFluc-WT

Plasmid

#177714

Purpose

Cytosolic firefly luciferase reporter previously described in Shcherbakov et al. 2019

Depositor

Erik Böttger

Article

Shcherbakov et al Commun Biol. 2019 Oct 17;2:381. doi: 10.

Insert

hFluc WT

Expression

Bacterial and Mammalian

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pSelect-6

Plasmid

#190243

Purpose

Encodes lacI- and GFP-targeting gRNAs for positive and negative selections of dCas9 activity.

Depositor

Marc Ostermeier

Article

Spisak et al PLoS One. 2022 Jun 3;17(6):e0269270. doi

Insert

LacI

Expression

Bacterial

Available Since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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