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pZac2.1 gfaABC1D-Lck-13xLinker-BioID2-BioID2-HA

Plasmid

#176741

Purpose

Expresses membrane bound biotin ligase, BioID2, in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Lck-13xLinker-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET19b Adrm1

Plasmid

#19423

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

adhesion regulating molecule 1 (ADRM1 Human)

Tags

His

Expression

Bacterial

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
mTFP1-pBAD

Plasmid

#54553

Purpose

Localization: Protein Expression Vector , Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al Biochem J. 2006 Dec 15. 400(3):531-40.

Type

Empty backbone

Tags

6xHis and mTFP1

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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Pcr4-12xMBS-PBS

Plasmid

#52984

Purpose

provide MBS-PBS alternating sequence

Depositor

Robert Singer

Article

Wu et al Sci Rep. 2014 Jan 9;4:3615. doi: 10.1038

Insert

12xMBS-PBS

Expression

Bacterial

Promoter

none

Available Since

June 23, 2014

Availability

Academic Institutions and Nonprofits only

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pBAD-smURFP-RBS-HO-1

Plasmid

#80341

Purpose

Expresses smURFP and Heme Oxygenase-1 in bacterial cells.

Depositor

Erik Rodriguez, Roger Tsien

Article

Rodriguez et al Nat Methods. 2016 Aug 1. doi: 10.1038/nm

Inserts

smURFP
HO-1

Tags

6 Histidine Tag

Expression

Bacterial

Promoter

pBAD

Available Since

Aug. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-FnCas12a-TEV

Plasmid

#174658

Purpose

FnCas12a expression plasmid

Depositor

Jin Wang, Huimin Zhao

Article

Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

Insert

FnCas12a

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 4, 2021

Availability

Academic Institutions and Nonprofits only

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ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain

Plasmid

#228663

Purpose

Bacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)

Depositor

ASAP Discovery, Frank von Delft

Insert

Zika virus portion of NS2B protease coexpressed with NS3 protease

Tags

His-GST-TEV

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGPD2

Plasmid

#43972

Depositor

Hal Alper

Article

Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial and Yeast

Promoter

GPD

Available Since

April 9, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pJMP1337

Plasmid

#119270

Purpose

for cloning new sgRNAs

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pTP223

Plasmid

#13263

Depositor

Kenan Murphy

Article

Murphy et al BMC Mol Biol. 2003 Dec 13;4:11. doi: 10.

Insert

Plac-gam-bet-exo

Expression

Bacterial

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pGEX-2T Crkl

Plasmid

#36400

Depositor

Nora Heisterkamp

Article

ten Hoeve et al Cancer Res. 1994 May 15;54(10):2563-7.

Insert

Crkl (CRKL Human)

Tags

GST

Expression

Bacterial

Available Since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
pRU1156

Plasmid

#14473

Depositor

Philip Poole

Article

Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

Insert

Gfp-mut3.1-GusA

Expression

Bacterial

Available Since

April 23, 2007

Availability

Academic Institutions and Nonprofits only

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pKJ1712

Plasmid

#21870

Depositor

Keith Joung

Article

Maeder et al Nat Protoc. 2009. 4(10):1471-501.

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 3, 2009

Availability

Academic Institutions and Nonprofits only

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pET30b-5M SrtA with TEV site

Plasmid

#86962

Purpose

Expresses calcium-dependent Sortase A with a TEV cleavage site proximal to the C-terminal histidine tag

Depositor

Ron Bose

Article

Sarpong et al Anal Biochem. 2017 Mar 15;521:55-58. doi

Insert

S.aureus SrtA 5M

Tags

6x Histidine tag and TEV site

Expression

Bacterial

Mutation

P94R, D160N, D165A, K190E, K196T

Promoter

T7

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pJKR-L-tetR

Plasmid

#62562

Purpose

Tetracycline biosensor. Low copy plasmid that expresses GFP proportional to tetracycline concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-NanoLuc

Plasmid

#188313

Purpose

Expression of NanoLuc via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

NanoLuc

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAD-mKate(KPS12)

Plasmid

#133831

Purpose

Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides.

Depositor

Oscar Kuipers

Article

Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

Insert

mKate(KPS12)

Use

E.coli/bacillus spp. shuttle vector

Expression

Bacterial

Promoter

Pupp

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pKL09-GCaMP6f-mScarlet bb118

Plasmid

#158979

Purpose

Constitutive expression (sigma 70 promoter) of GCaMP6f tethered to mScarlet fluorophores in E. coli.

Depositor

Joel Kralj

Article

Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

Insert

GCaMP6f-mScarlet fusion

Expression

Bacterial

Mutation

codon optimized for e. coli from Addgene#100833

Promoter

Bba_J23118 (sigma 70)

Available Since

Sept. 17, 2020

Availability

Academic Institutions and Nonprofits only

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Xla.Tubb2:hGCGR-TEVcs-QF;he1.1:CFP

Plasmid

#218857

Purpose

Xla.Tubb2 promoter driving expression of human glucagon receptor, TEV protease recognition site, QF fusion protein for use in trans-Tango.

Depositor

Marnie Halpern

Article

Coomer et al Nat Neurosci. 2025 Jan;28(1):189-200. do

Insert

hGCGR (GCGR Human)

Tags

QF fusion protein

Expression

Bacterial

Available Since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pKL004

Plasmid

#98920

Purpose

Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.

Depositor

Joel Kralj

Article

Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11

Insert

GCaMP6f tethered to mRuby

Tags

His and mRuby3

Expression

Bacterial

Promoter

BBa_J23118 - Strong Constitutive

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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