30,576 results

1. pNCST-AvicFP3

   Plasmid

   #129506

   Purpose

   Expresses AvicFP3 constitutively in E. coli (most strains)

   Depositor

   Nathan Shaner

   Article

   Lambert et al PLoS Biol. 2020 Nov 2;18(11):e3000936. d

   Insert

   AvicFP3
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   synthetic constitutive (stationary phase) promote…

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJUMP49-2D'(sfGFP)

   Plasmid

   #126994

   Purpose

   Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJUMP29-1D'(sfGFP)

   Plasmid

   #126981

   Purpose

   Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-Sfp R4-4 mutant

   Plasmid

   #173491

   Purpose

   Expression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.

   Depositor

   Jun Yin

   Article

   Sunbul et al J Mol Biol. 2009 Apr 10;387(4):883-98. d

   Insert

   Sfp-R44
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. SPLICSFAST-ER-PO-P2A

   Plasmid

   #214894

   Purpose

   Expresses equimolar Peroxisome- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-PO Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-ER-PO-P2A
   

   Use

   Tags

   none

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQIq_MRGS_HIS8_(27F3)_FLAG

   Plasmid

   #199615

   Purpose

   Encodes DARPin-FLAG 27F3 for bacterial expression and column purification

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   DARPin-FLAG 27F3
   

   Use

   Tags

   8xHis and FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available Since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPhiC31o

   Plasmid

   #13794

   Purpose

   Expression of mouse codon-optimized PhiC31 recombinase

   Depositor

   Philippe Soriano

   Article

   Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

   Insert

   PhiC31o
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. CH-GECO2.1

   Plasmid

   #52099

   Purpose

   mCherry-based Calcium indicators

   Depositor

   Robert Campbell

   Article

   Carlson et al Protein Eng Des Sel. 2013 Dec;26(12):763

   Insert

   CH-GECO2.1
   

   Use

   Tags

   6 His tag

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD promotor

   Available Since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pGEX-4T1-RhoA G17A

   Plasmid

   #69357

   Purpose

   expression of nucleotide free RhoA in bacterial cells

   Depositor

   Rafael Garcia-Mata

   Article

   Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

   Insert

   RhoA (RHOA Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   G17A

   Promoter

   tac

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

10. p006-GFP-strong

    Plasmid

    #108313

    Purpose

    GFP from jellyfish Aquorea sp. under strong synthetic constitutive promoter pFAB4026; kanamycin resistant.

    Depositor

    Jaroslaw Bryk

    Article

    Siddall et al bioRxiv 2021.06.20.449138

    Insert

    Green fluorescent protein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pFAB4026

    Available Since

    Aug. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGRB

    Plasmid

    #71539

    Purpose

    for expression of gRNA in E. coli

    Depositor

    Tao Chen

    Article

    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J231196

    Available Since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKL10-mRuby-iATPSnFR1.0 bb118

    Plasmid

    #158980

    Purpose

    Constitutive expression (sigma 70 promoter) of the ratiometric mRuby-iATPSnFR1.0 ATP indicator in E. coli.

    Depositor

    Joel Kralj

    Article

    Bruni et al eLife. 2020 Aug 4;9. pii: 58706. doi: 10

    Insert

    mruby-iATPSNfR1.0
    

    Use

    Tags

    mruby3

    Expression

    Bacterial

    Mutation

    codon optimized for e. coli from Addgene#102557

    Promoter

    Bba_J23118 (sigma 70)

    Available Since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNC02

    Plasmid

    #233644

    Purpose

    For bacterial expression of sfGFP c-terminally tagged with VitelloTagB.

    Depositor

    Zak Swartz

    Article

    Clarke et al Development. 2024 Oct 15;151(20):dev2028

    Insert

    sfGFP with c-terminal VitelloTagB
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-hDcp2

    Plasmid

    #72214

    Purpose

    bacterial expression of His-tagged human Dcp2

    Depositor

    Megerditch Kiledjian

    Article

    Wang et al Proc Natl Acad Sci U S A. 2002 Oct 1;99(

    Insert

    Dcp2 (DCP2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pEVOL-pylT-AznLRS

    Plasmid

    #172482

    Purpose

    For E.Coli expression, 306L309L348I384F411W

    Depositor

    Wenshe Liu

    Article

    Wang et al Angew Chem Int Ed Engl. 2017 Feb 1;56(6)

    Insert

    AznLRS and pylTcua
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available Since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET264-pUC 24xMS2V6 Loxp KANr Loxp

    Plasmid

    #104393

    Purpose

    Yeast endogenous mRNA tagging

    Depositor

    Robert Singer, Evelina Tutucci

    Article

    Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

    Insert

    24xMS2V6
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    All Loops are U-variants, interspaced by 50 nucle…

    Promoter

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pG106 - KA

    Plasmid

    #178041

    Purpose

    Bacteroides - Escherichia coli shuttle vector with ErmF and ErmAM, Kan and Amp selection markers

    Depositor

    Janina Lewis

    Article

    Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

    Type

    Empty backbone

    Use

    Shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 1, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pY001 (pFnCpf1_full)

    Plasmid

    #69973

    Purpose

    Expresses FnCpf1 locus including Cas genes and spacers 1-4 of CRISPR array.

    Depositor

    Feng Zhang

    Article

    Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

    Insert

    FnCpf1 locus
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. 1B-GFP1-10

    Plasmid

    #193867

    Purpose

    E. coli expression vector for Hisx6 tagged GFP1-10.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    sfGFP1-10
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available Since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBT1-proD-mCherry

    Plasmid

    #65823

    Purpose

    Const. mCherry expression , proD promoter, E. coli Expression

    Depositor

    Michael Lynch

    Insert

    mCherry
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    proD E . coli Constitutive Promoter

    Available Since

    July 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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