32,127 results

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PAHA

Plasmid

#74663

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

PKU1 (PAH Human)

Tags

His-6-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2016

Availability

Academic Institutions and Nonprofits only
pMS23: pHelper(PmcCAST)_entry_ΔTnsD

Plasmid

#168156

Purpose

Inducible expression of PmcCAST proteins (except TnsD). Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

PmcCAST minimal CRISPR array
PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
PmcCAST Tns proteins (TnsAB and TnsC)
PmcTniQ

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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3269 pBS Bcl-2

Plasmid

#8764

Depositor

Stanley Korsmeyer

Article

Silverman et al Proc Natl Acad Sci U S A 1990 Dec;87(24)

Insert

Bcl-2 (Bcl2 Mouse)

Expression

Bacterial

Available Since

July 6, 2005

Availability

Academic Institutions and Nonprofits only
pGex-6p2_ATRX

Plasmid

#221377

Purpose

Expresses ATRX in bacterial cells

Depositor

Albert Jeltsch

Article

Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

Insert

Transcriptional regulator ATRX (ATRX Human)

Expression

Bacterial

Promoter

tac

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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prp8-G736E

Plasmid

#108450

Purpose

prp8-G736E in pRS313 backbone for expression in yeast

Depositor

Christine Guthrie

Article

Mayerle et al Proc Natl Acad Sci U S A. 2019 Feb 5;116

Insert

PRP8 (PRP8 Budding Yeast)

Expression

Bacterial and Yeast

Mutation

changed G736 to E

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2 C450A

Plasmid

#81031

Purpose

Mammalian expression of the dark state of LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Mutation

C450A

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pBTB-4

Plasmid

#22820

Depositor

Ryan Gill

Article

Lynch et al Biotechnol Bioeng. 2006 May 5. 94(1):151

Type

Empty backbone

Expression

Bacterial

Available Since

March 22, 2010

Availability

Academic Institutions and Nonprofits only

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pFTK036

Plasmid

#171308

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

amdS ANIA_08777 acetamidase, selection marker

Use

Synthetic Biology

Expression

Bacterial and Yeast

Mutation

n/a

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-HsSENP3(307-574)

Plasmid

#71481

Purpose

Encodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 3 (SENP3 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Deleted amino acids 1-306 (catalytic domain only)

Promoter

T7

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only
pdCas9-AsiA

Plasmid

#158063

Purpose

Expressing CasTA of dCas9 fused AsiA

Depositor

Harris Wang

Article

Ho et al Mol Syst Biol. 2020 Jul;16(7):e9427. doi

Insert

dCas9-AsiA

Expression

Bacterial

Promoter

TetO

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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