30,260 results

1. pGDP1 NDM-1

   Plasmid

   #112883

   Purpose

   This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

   Depositor

   Gerard Wright

   Article

   Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

   Insert

   NDM-1 (blaNDM-1 Klebsiella pneumoniae)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Pbla

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pramr-GFP

   Plasmid

   #194148

   Purpose

   Contains a RamR-regulated promoter expressing GFP

   Depositor

   Andrew Ellington

   Article

   d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

   Insert

   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pramr

   Available since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. GST PTBP1 isoform 4

   Plasmid

   #108594

   Purpose

   Recombinant GST tagged protein production

   Depositor

   Chris Smith

   Article

   Coelho et al EMBO J. 2015 Mar 4;34(5):653-68. doi: 10

   Insert

   PTBP1 isoform 4 (PTBP1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   isoform 4 (isoform a)

   Promoter

   tac

   Available since

   Dec. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJCSUP35

   Plasmid

   #1088

   Depositor

   Susan Lindquist

   Article

   Glover et al Cell 1997 May 30;89(5):811-9.

   Insert

   SUP35 (SUP35 Budding Yeast)
   

   Use

   Tags

   HIS tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 26, 2005

   Availability

   Academic Institutions and Nonprofits only

5. PARP9-c020

   Plasmid

   #173097

   Purpose

   Expression of recombinant fusion protein in E. coli

   Depositor

   Lari Lehtiö

   Article

   Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

   Insert

   PARP9 (PARP9 Human)
   

   Use

   Tags

   mCerulean

   Expression

   Bacterial

   Mutation

   N310-N493

   Promoter

   Available since

   Nov. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDx_mScarlet-I3-SYFP2

   Plasmid

   #189763

   Purpose

   Dual expression vector for bacteria and mammalian cells producing mScarlet-I3-SYFP2 fusion protein for FRET analysis

   Depositor

   Dorus Gadella

   Article

   Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

   Insert

   mScarlet-I3-SYFP2
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV & Rha

   Available since

   March 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. GST-thr-RAF1-RBD

   Plasmid

   #86033

   Purpose

   Expresses the RAS-binding domain of RAF1 for in vitro analysis of RAS activation

   Depositor

   Dominic Esposito

   Insert

   RAF1 RBD (Ras-binding domain) (RAF1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Contains residues 1-149

   Promoter

   T7

   Available since

   Jan. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. Luciferase

   Plasmid

   #213979

   Purpose

   Expresses Doxy Inducible Luciferase

   Depositor

   Coleman Lindsley

   Article

   Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

   Insert

   Luciferase
   

   Use

   Lentiviral

   Tags

   6xHis affinity tag

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Tet-responsive promoter PTight, consisting of sev…

   Available since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBAD-LacI

   Plasmid

   #46394

   Purpose

   Overproduction of LacI

   Depositor

   Florian Hollfelder

   Article

   Gatti-Lafranconi et al Microb Cell Fact. 2013 Jul 8;12:67. doi:

   Insert

   LacI
   

   Use

   Tags

   6xHIS tag

   Expression

   Bacterial

   Mutation

   silent T to C mutation at position 19

   Promoter

   arabinose promoter

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. phage UbiC SNAP-DDX6

    Plasmid

    #119948

    Purpose

    Lentiviral vector expressing SNAP-DDX6 fusion protein

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    DDX6 (DDX6 Human)
    

    Use

    Lentiviral

    Tags

    SNAP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    UbiC

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-Duet-1-NONO(53-312)

    Plasmid

    #135442

    Purpose

    Expresses NONO 53-312 in the second site; for co-expression with His-tagged protein

    Depositor

    Charles Bond

    Article

    Lee et al Nucleic Acids Res. 2015 Apr 20;43(7):382

    Insert

    Non-POU domain-containing octamer-binding protein (NONO Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTriEx-mCherry-PA-Rac1-C450A

    Plasmid

    #22028

    Purpose

    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
    

    Use

    Tags

    6xHis and mCherry

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…

    Promoter

    CMV, p10

    Available since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMMB67EH-CFP

    Plasmid

    #90103

    Purpose

    CFP control expression plasmid

    Depositor

    Samuel Miller

    Article

    Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

    Insert

    CFP
    

    Use

    Broad-host range

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTXBI-mclover-YTHDC1-FL

    Plasmid

    #177121

    Purpose

    Bacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    YTHDC1 (YTHDC1 Human)
    

    Use

    Tags

    Mxe GyrA intein-Chitin binding domain for protein…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-SENP1 (catalytic domain)

    Plasmid

    #16356

    Depositor

    Guy Salvesen

    Article

    Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

    Insert

    human SENP1 catalytic domain (SENP1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Amino Acid 419 to 644 catalytic domain of human S…

    Promoter

    Available since

    Dec. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pnsgRNA

    Plasmid

    #172717

    Purpose

    Delivery of guide RNA for prime editing

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    J23119-gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pC0057 BzoCas13 (B01) His6-TwinStrep-SUMO-BsaI

    Plasmid

    #115207

    Purpose

    Expresses BzoCas13b from a SUMO-tagged bacterial expression construct

    Depositor

    Feng Zhang

    Article

    Gootenberg et al Science. 2018 Apr 27;360(6387):439-444.

    Insert

    BzoCas13
    

    Use

    Tags

    SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET_RTX-6xHis

    Plasmid

    #145029

    Purpose

    pET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tag

    Depositor

    Andrew Ellington

    Article

    Bhadra et al BioProtocol 2021

    Insert

    RTX
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNIC28-BSA4-G-L11

    Plasmid

    #127830

    Purpose

    Production of recombinant human ribosomal protein L11

    Depositor

    Ivanhoe Leung

    Article

    Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

    Insert

    RPL11 (RPL11 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMAL-his-LbCpf1-EC

    Plasmid

    #79008

    Purpose

    Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

    Depositor

    Jin-Soo Kim

    Article

    Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

    Insert

    LbCpf1
    

    Use

    Tags

    MBP-6xHis-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    tac promoter

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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