33,902 results
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Plasmid#13577DepositorInsertRat liver fatty acid binding protein (Fabp1 Rat)
ExpressionBacterialAvailable sinceMarch 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pMK47
Plasmid#13324DepositorInsertgyrB
ExpressionBacterialAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRS314-URA3
Plasmid#11004DepositorAvailable sinceApril 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBFC0984
Plasmid#231992PurposeCRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning siteDepositorInsertsdRfxCas13d
crRNA with 2xBsaI spacer
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119 and pTetAvailable sinceApril 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
CRBN_ΔHBD
Plasmid#232779PurposeExpresses CRBN_ΔHBD sequence in E. coli cells with cleavable MBP-His tag and deleted internal DDB1 binding domainDepositorInsertCereblon (CRBN Human)
TagsMBP-His6-TEVExpressionBacterialMutationCRBN (residues 47 to 193 and 249 to 436) with GNG…PromoterT7Available sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRMC2-msfGFP
Plasmid#194913PurposeTetracycline inducible expression of msfGFP in StaphylococciDepositorInsertShine Dalgarno Sequence followed by monomeric superfolder GFP
UseTetracycline-inducible expression vector for stap…ExpressionBacterialAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBZS-M.CviPI
Plasmid#198355PurposeExpress M.CviPI in bacterial cells.DepositorInsertM.CviPI (M.CviPI )
ExpressionBacterialAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBK- Ma PylRS WT
Plasmid#197573PurposeExpresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.DepositorInsertM. alvus PylRS WT
ExpressionBacterialPromoterGlnS (constitutive)Available sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
RepX_pET28a(+)
Plasmid#172199PurposeExpresses 6xHis-tagged RepX in E. coli BL21(DE3), kanamycin resistance, optimal growth at 20 degrees.DepositorInsertRepX superhelicase
Tags6xHis tagExpressionBacterialMutationC18L, C43S, C167V, S400C, C612AAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAT-WT-Myc
Plasmid#177717PurposeER protein alpha-1-antitrypsin (WT), myc taggedDepositorAvailable sinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pTO149_RFP_CauNEO
Plasmid#177277PurposeEncodes a G418 (NEOr) selectable marker with codon-optimization for expression in CUG clade fungi. Also encodes RFP tagging of Candida auris ENO1 to measure transformation efficiency.DepositorInsertRFP, CauNEO
TagsENO1-RFPExpressionBacterialMutationAll CUG Leucine codons were replaced with the syn…PromoterAgTEF2Available sinceNov. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-NTD
Plasmid#171235PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR N-terminal-domain (NTD)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert2xHA-tagged human androgen receptor trancate: N-terminal-domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p70a-T7RNAP
Plasmid#105683PurposeExpresses T7 polymerase in cell-free TXTL.DepositorInsertT7 polymerase
ExpressionBacterialPromoterOR2-OR1-Pr (bacteriophage Lambda with one mutatio…Available sinceMay 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE-60NA-proinsulin-msGFP2
Plasmid#160466PurposeEncodes a fusion of human proinsulin and the monomeric superfolder GFP2DepositorInsertproinsulin-msGFP2
ExpressionBacterialPromoterT5Available sinceMarch 10, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJV53-Cas12a
Plasmid#158706PurposeCRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61DepositorInsertFnCpf1
UseCRISPRExpressionBacterialPromoterTetOAvailable sinceJan. 15, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLG007 (Ec86 retron)
Plasmid#157885PurposeExpresses the Ec86 retron defense systemDepositorInsertEc86 retron
ExpressionBacterialAvailable sinceDec. 21, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
bNS2B47NS3
Plasmid#155320Purposeco-expression of DENV4 47 peptides from NS2B cofactor and NS3 full lengthDepositorInsertCo expression of DENV4 NS3 full length protein with 47 peptides from NS2B cofactor
TagsHis tagExpressionBacterialMutationWTPromoterT7Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUD-MSP1D1
Plasmid#161608PurposeBacterial expression of mutated membrane scaffold protein ("ultra-dark" MSP1D1) for Nanodisc formationDepositorInsertUD-MSP1D1
Tags7-His tagExpressionBacterialMutationmutant of MSP1D1; all tryptophans and tyrosines w…PromoterT7Available sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GEF-H1 FLARE212.pa
Plasmid#134930PurposeGEF-H1 biosensor that does not have GEF activity.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)
TagsNone.ExpressionBacterial and MammalianPromoterCMV and T7Available sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only