30,738 results

1. 40 pCOLI_K_MBP

   Plasmid

   #160183

   Purpose

   N-term MBP 3C cleavable Kan resistant bacterial expression vector

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX6P1‐hsRNASEH2BCA

   Plasmid

   #108692

   Purpose

   For expression in E coli of N-terminally GST-tagged human RNASEH2B and untagged RNASEH2C and A, allowing affinity purification of the RNase H2 trimeric enzyme

   Depositor

   Martin Reijns, Andrew Jackson

   Article

   Reijns et al J Biol Chem. 2011 Mar 25;286(12):10530-9

   Inserts

   RNASEH2B (RNASEH2B Human)
   RNASEH2C (RNASEH2C Human)
   RNASEH2A (RNASEH2A Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Shine Dalgarno sequence upstream of start codon a…

   Promoter

   tac

   Available Since

   April 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDC158

   Plasmid

   #115793

   Purpose

   Expresses Rat SNAP25

   Depositor

   Axel Brunger

   Article

   Lai et al Neuron. 2017 Aug 2;95(3):591-607.e10. do

   Insert

   SNAP25 (Snap25 Rat)
   

   Tags

   hexa histidine

   Expression

   Bacterial

   Mutation

   c84s, c85s, c90s, c92s

   Promoter

   T7

   Available Since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFPV25.1

   Plasmid

   #20668

   Purpose

   S. typhimurium rpsM promoter driving gfpmut3 expression in bacteria. Promoter is not regulated by acidic conditions.

   Depositor

   Raphael Valdivia

   Article

   Valdivia et al Mol Microbiol. 1996 Oct . 22(2):367-78.

   Type

   Empty backbone

   Tags

   GFP mut3

   Expression

   Bacterial

   Available Since

   March 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. YARS_pNIC-CTB10H

   Plasmid

   #153059

   Purpose

   Expression construct for producing Avi tagged Tyrosyl-tRNA synthetase

   Depositor

   Susanne Gräslund

   Insert

   YARS (YARS1 Human)
   

   Tags

   TEV-Avi-His10

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. OPEN Beta2-microglobulin

   Plasmid

   #215657

   Purpose

   E. coli expression of human beta-2-microglobulin containing a cysteine mutation for disulfide linkage to mutant HLA.

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN Beta2-microglobulin (B2M Human)
   

   Expression

   Bacterial

   Mutation

   Histidine 31 to Cysteine

   Promoter

   T7

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX-EZH2

   Plasmid

   #28060

   Depositor

   Mien-Chie Hung

   Article

   Wei et al Nat Cell Biol. 2011 Jan . 13(1):87-94.

   Insert

   EZH2 (EZH2 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   June 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. esmGFP-C10-pVTCB052

   Plasmid

   #231303

   Purpose

   Plasmid used for expression of AI-designed GFP protein esmGFP-C10 E. coli

   Depositor

   Alexander Rives

   Insert

   esmGFP-C10
   

   Expression

   Bacterial

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX4T1-Rpb4/7

   Plasmid

   #138484

   Purpose

   Expresses GST-tagged Rpb4 and His-tagged Rpb7 in bacteria

   Depositor

   Britt Glaunsinger

   Article

   Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

   Inserts

   Rpb4 (POLR2D Human)
   Rpb7 (POLR2G Human)
   

   Tags

   6xHis and GST

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDR111_Bgal no NLS

    Plasmid

    #188397

    Purpose

    Expresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.

    Depositor

    Christopher Contag

    Article

    Madsen et al Commun Biol. 2022 Aug 30;5(1):888. doi:

    Insert

    lacZ
    

    Tags

    PhoD secretion peptide

    Expression

    Bacterial

    Promoter

    Phyper spank

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pETDuet-T7-Ct-YhjH

    Plasmid

    #79167

    Purpose

    Dual expression of Ct biosensor and YhjH under individual T7 promoters

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Inserts

    yhjH
    T7-Spinach2-Ct
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET30-HPF1-His-Sumo-Flag

    Plasmid

    #111577

    Purpose

    Expresses full length HPF1 in E. coli

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    HPF1 (HPF1 Human)
    

    Tags

    6xHis-Sumo-FLAG

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pQE30-His-RF3

    Plasmid

    #124131

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Release factor 3 (prfC Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGPD2

    Plasmid

    #43972

    Depositor

    Hal Alper

    Article

    Crook et al Nucleic Acids Res. 2011 Aug;39(14):e92.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial and Yeast

    Promoter

    GPD

    Available Since

    April 9, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pGEx-4T-1-YTHDF1

    Plasmid

    #70087

    Purpose

    Encode YTHDF1 with N-terminal GST tag for E. coli expression

    Depositor

    Chuan He

    Article

    Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

    Insert

    YTH N(6)-methyladenosine RNA binding protein 1 (YTHDF1 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Nov. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCP30T-E2Crimson

    Plasmid

    #78478

    Purpose

    Broad range bacterial fluorescence labeling

    Depositor

    Mariette Barbier

    Article

    Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

    Insert

    E2-Crimson
    

    Expression

    Bacterial

    Available Since

    June 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1.Gαi1-LgB91

    Plasmid

    #134342

    Purpose

    Nanoluc complementation assay. Expression of Gαi1 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 91 and 92 of Gαi1. Addition of the HA epitope at N terminus of Gαi1.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαi1-LgB91 (GNAI1 Human)
    

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Promoter

    T7

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. GST-GRP78-Full Length (pGEX4T1)

    Plasmid

    #238421

    Purpose

    Expresses GRP78 Full Length fused to the GST Tag

    Depositor

    Amy Shiu Lee

    Article

    Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. 73-21 KRAS PANCS-binder posAP

    Plasmid

    #240119

    Purpose

    KRAS G12D PANCS-binder positive AP

    Depositor

    Bryan Dickinson

    Article

    Styles et al bioRxiv [Preprint]. 2025 Jan 17:2025.01.

    Insert

    KRas-RNAPC Pos AP fusion
    

    Expression

    Bacterial

    Promoter

    custom

    Available Since

    July 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRPCN

    Plasmid

    #193726

    Purpose

    Expresses Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection

    Depositor

    Martin Buck

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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