29,729 results

1. pET51b_HCAII

   Plasmid

   #187110

   Purpose

   Expression of HCAII in bacteria

   Depositor

   Kai Johnsson

   Article

   Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

   Insert

   HCAII
   

   Use

   Tags

   Hisx10 and Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCFT4.2

   Plasmid

   #208807

   Purpose

   Expresses the fluorescent protein phiLOV2.1

   Depositor

   François-Xavier Campbell-Valois

   Article

   Tchagang et al Front Microbiol. 2023 Oct 20;14:1245755.

   Insert

   phiLOV2.1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Oct. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-16b-10His-TEV-MCES_VACCA_D1_D12

   Plasmid

   #203671

   Purpose

   The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

   Depositor

   Stephen Cusack

   Article

   Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

   Insert

   MCES_VACCA_D1_D12
   

   Use

   Tags

   10xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTKW106alp7A

   Plasmid

   #69360

   Purpose

   Stabilized lacZ expression vector containing alp7A and hok/sok

   Depositor

   Jeff Hasty

   Article

   Danino et al Sci Transl Med. 2015 May 27;7(289):289ra

   Inserts

   lacZ
   alp7A
   hok
   placIq-lacI
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTac and placIq

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDule- Mj Acd A9

   Plasmid

   #197651

   Purpose

   E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair

   Depositor

   Ryan Mehl

   Article

   Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-

   Inserts

   Mj Acd A9 synthetase
   Methanocaldococcus jannaschii tRNA
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32A, L65D, F108L, Q109E, D158S, L162T

   Promoter

   GlnS and lpp

   Available since

   April 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pFLxR3

   Plasmid

   #167518

   Purpose

   Broad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Tetracycline Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   LuxR-mRFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PLuxB

   Available since

   Oct. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. KE-005

   Plasmid

   #133440

   Purpose

   Human elF4A1 residues 1-406, N-term His tag, thrombin cleavage, in pET21 vector backbone.

   Depositor

   Gerhard Wagner

   Insert

   EIF4A1 (EIF4A1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSC101-pT7-T3RNAP

   Plasmid

   #140872

   Purpose

   T7 promoter driven synthesis of T3 RNA polymerase

   Depositor

   Neal Devaraj

   Article

   Niederholtmeyer et al Nat Commun. 2018 Nov 28;9(1):5027. doi:

   Insert

   T3 RNA polymerase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEx-4T-1-YTHDF3

   Plasmid

   #70088

   Purpose

   Encode YTHDF3 isoform a with N-terminal GST tag for E. coli expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pBTR1

    Plasmid

    #22468

    Depositor

    Gary Pielak

    Article

    Olteanu et al Biochem Biophys Res Commun. 2003 Dec 19.

    Insert

    cytochrome c (CYCS Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCDF-His-mTET1CD

    Plasmid

    #81053

    Purpose

    bacterial expression of the catalytic domain of murine TET1

    Depositor

    Primo Schaer

    Article

    Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

    Insert

    TET1 (Tet1 Mouse)
    

    Use

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    catalytic domain amino acid 1367-2057

    Promoter

    T7

    Available since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBEST-p15a-pBAD-UTR1-TetR-T500

    Plasmid

    #45787

    Depositor

    Richard Murray

    Insert

    Tetracycline repressor
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pBLADE_ONLY_C

    Plasmid

    #168050

    Purpose

    Expresses the synthetic gene BLADE FP6 under the constitutive J23101** promoter.

    Depositor

    Barbara Di Ventura

    Article

    Romano et al Nat Chem Biol. 2021 Apr 26. pii: 10.1038

    Insert

    Blue light-inducible AraC dimers in Escherichia coli
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23101**

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pST-KT

    Plasmid

    #44561

    Purpose

    for ectopic inducible expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    UV15 promoter

    Available since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-6xHis-MBP-TEV site-TadA8e

    Plasmid

    #208319

    Purpose

    Overxpression MBP-TadA8e

    Depositor

    Weixin Tang

    Article

    Xiao et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    TadA8e
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    mutations in TadA are described in the paper

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCH1/RAD51o

    Plasmid

    #102562

    Purpose

    Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon

    Depositor

    Maria Spies

    Article

    Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11

    Inserts

    Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Human, Synthetic)
    E. coli groE operon
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E.coli Expression

    Promoter

    T7

    Available since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. tracrRNA(scr)-CJ8421_04975 mRNA

    Plasmid

    #199718

    Purpose

    Expresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequence

    Depositor

    Chase Beisel

    Article

    Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

    Insert

    CJ8421_04975 mRNA and scrambled tracrRNA
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21a-C2-SNAP

    Plasmid

    #208769

    Purpose

    To express C2-SNAP in bacteria

    Depositor

    Urte Neniskyte

    Article

    Dirvelyte et al Cell Mol Biol Lett. 2023 Jul 27;28(1):59

    Insert

    C2-SNAP (Mfge8 Mouse)
    

    Use

    Tags

    SNAP-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. CD Ugi4

    Plasmid

    #177196

    Purpose

    Greengate entry vector pGGC000 modified to contain bladderwort insulator 4

    Depositor

    Wayne Parrott

    Article

    Park et al bioRxiv 2021.11.04.467280

    Insert

    Utricularia gibba insulator 4
    

    Use

    Tags

    Expression

    Bacterial and Plant

    Mutation

    Promoter

    Available since

    June 28, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pcDNA3.1.Gαs-LgB113

    Plasmid

    #134362

    Purpose

    Nanoluc complementation assay. Expression of Gαs protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 113 and 114 of Gαs. Addition of the HA epitope at N terminus of Gαs.

    Depositor

    Julien Hanson

    Article

    Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

    Insert

    Gαs-LgB113 (GNAS Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    T7

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge