30,416 results

1. pET Flag TEV LIC cloning vector (2L-T)

   Plasmid

   #29715

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   FLAG-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET-51b(+)_NADP-Snifit

   Plasmid

   #113913

   Purpose

   Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+

   Depositor

   Kai Johnsson

   Article

   Sallin et al eLife. 2018 May 29;7. pii: 32638. doi: 1

   Insert

   NADP-Snifit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Sept. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC-GFP-AT

   Plasmid

   #133306

   Purpose

   "Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisation

   Depositor

   Chris Barnes

   Article

   Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

   Insert

   axe/txe toxin-antitoxin cassette
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. sgRNA_expression_vector

   Plasmid

   #210212

   Purpose

   An empty gRNA expression vector to clone U6 promoter driven sgRNAs with gRNA scaffold and with co-expression of DsRed. sgRNA can be cloned by Golden Gate Assembly or restriction digestion using BbsI.

   Depositor

   Albert Jeltsch

   Article

   Rajaram et al Methods Mol Biol. 2024;2842:179-192. doi

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-4T-1-3xFLAG

   Plasmid

   #129570

   Purpose

   Empty backbone for FLAG tag

   Depositor

   Kevin Janes

   Article

   Smolko et al Sci Rep. 2019 Dec 19;9(1):19409. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Jan. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. B23(9-122)

   Plasmid

   #23142

   Depositor

   SeWon Suh

   Article

   Lee et al Proteins. 2007 Nov 15. 69(3):672-8.

   Insert

   B23 core (NPM1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-8

   Promoter

   Available Since

   Sept. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pEvolvR-enCas9-PolI5M

   Plasmid

   #113078

   Purpose

   Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   John Dueber, David Schaffer

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI5M
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRibo-Sec-APEX2m

   Plasmid

   #176844

   Purpose

   Expression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)

   Depositor

   Jessica Seeliger

   Article

   Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

   Insert

   pRibo-Sec-APEX2m
   

   Use

   Inducible

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pRibo, theophilline riboswitch inducible hsp60 pr…

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHIS2-TIP47 (Delta 1-190)

   Plasmid

   #17640

   Depositor

   James Hurley

   Article

   Hickenbottom et al Structure. 2004 Jul . 12(7):1199-207.

   Insert

   Tip47 (Delta 1-190) (Plin3 Mouse)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Delta 1-190

   Promoter

   Available Since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pET28-iLOVf

    Plasmid

    #63723

    Purpose

    E coli expression plasmid to produce Thrombin cleavable His tagged iLOV fusion proteins

    Depositor

    Brian Smith

    Type

    Empty backbone

    Use

    Tags

    TEE, iLOV, 6xHis, thrombin cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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