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pQE60-EFTs-His

Plasmid

#124128

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Elongation factor Ts (tsf Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pGTM_COV2_NSP5_004_SUMO

Plasmid

#190062

Purpose

Expresses SARS-CoV-2 3CLpro for the expression of the active mature protease

Depositor

Gaetano Montelione

Article

Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

Insert

3CLpro (ORF1ab SARS-CoV-2)

Tags

6X-His-Sumo

Expression

Bacterial

Promoter

T7 lac

Available Since

June 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAGD248

Plasmid

#219498

Purpose

Temperature sensitive replication (repA101ts) backbone encoding the retron recombineering operon (ncRNA, RT, CspRecT, mutLE32K)

Depositor

Seth Shipman

Article

Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

Insert

Eco1 ncRNA (rpoB donor), Eco1 RT, CspRecT, mutLE32K

Expression

Bacterial

Promoter

pBAD

Available Since

May 31, 2024

Availability

Academic Institutions and Nonprofits only

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mEos3-Rac1

Plasmid

#92039

Purpose

Expresses mEos3-Rac1 fusion protein

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pMRE-Tn7-151

Plasmid

#118565

Purpose

miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

mTurquoise2

Use

Minitn7 delivery vector

Expression

Bacterial

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pCS6

Plasmid

#55752

Purpose

Produces T7 RNA Polymerase.

Depositor

Matthew Bennett

Article

Schmidt et al ACS Synth Biol. 2012 Oct 19;1(10):445-50

Insert

T7 RNA Polymerase

Expression

Bacterial

Promoter

pBAD

Available Since

July 15, 2014

Availability

Academic Institutions and Nonprofits only

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mEos3-dnRac1

Plasmid

#92040

Purpose

Expresses mEos3 fusion with dnRac1 (T17N).

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Mutation

T17N (dominant negative)

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_PDCD6_EF-hand

Plasmid

#110009

Purpose

Protein expression and purification of PDCD6_EF-hand

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PDCD6_EF-hand (PDCD6 Human)

Expression

Bacterial

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pScaf-5544.1

Plasmid

#111405

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v1)

Depositor

Shawn Douglas

Article

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-GH'

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-pgaC

Plasmid

#154919

Purpose

aTc inducible gRNA targeting pgaC along with arabinose-inducible lambda red enzymes

Depositor

Michael Lynch

Article

Moreb et al CRISPR J. 2020 Dec;3(6):550-561. doi: 10

Insert

gRNA targeting pgaC

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

pTet

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET9d-hnRNP-A1

Plasmid

#23026

Depositor

Douglas Black

Insert

hnRNPA1 (HNRNPA1 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 26, 2012

Availability

Academic Institutions and Nonprofits only

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pUCP20T-ecfp

Plasmid

#78463

Purpose

Broad range bacterial fluorescence labeling

Depositor

Mariette Barbier

Article

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

eCFP

Expression

Bacterial

Available Since

June 14, 2016

Availability

Academic Institutions and Nonprofits only

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ERG_pet28a

Plasmid

#131642

Purpose

Bacterial expression of ETS gene ERG

Depositor

Peter Hollenhorst

Article

Selvaraj et al Cell Commun Signal. 2015 Feb 19;13:12. d

Insert

ERG (ERG Human)

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pMSP1E3D1_D73C

Plasmid

#39328

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E3D1_D73C (APOA1 synthetic gene)

Tags

6xHis

Expression

Bacterial

Mutation

cys mutant of MSP1E3D1

Promoter

T7

Available Since

Sept. 11, 2012

Availability

Academic Institutions and Nonprofits only
pBK- Ma PylRS WT

Plasmid

#197573

Purpose

Expresses wild-type Methanomethylophilus alvus pyrrolysine tRNA synthetase under GlnS promoter. Used for Pyl tRNA-synthetase selections.

Depositor

Ryan Mehl

Article

Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

Insert

M. alvus PylRS WT

Expression

Bacterial

Promoter

GlnS (constitutive)

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCOLA-T7-WspR

Plasmid

#79162

Purpose

Expresses WspR WT under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

WspR (wspR P. areuginosa)

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pASK-mSAN1-Streptag

Plasmid

#117165

Purpose

Expresses Strep-tagged murine SAN1 nuclease in bacteria

Depositor

Ian Macara

Article

Andrews et al Nat Commun. 2018 Jul 3;9(1):2592. doi: 1

Insert

mSAN1 (Fam120b Mouse)

Tags

Streptag

Expression

Bacterial

Promoter

tetR/tetA

Available Since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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mEos3-caRac1

Plasmid

#92041

Purpose

Expresses mEos3 fusion with caRac1 (Q61L)

Depositor

Ji Yu

Article

Das et al Proc Natl Acad Sci U S A. 2015 Jan 20;11

Insert

RAC1 (RAC1 Human)

Tags

mEos3

Expression

Bacterial, Insect, and Mamm…

Mutation

Q61L (constitutively active)

Promoter

CMV

Available Since

June 8, 2017

Availability

Academic Institutions and Nonprofits only

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pDB6 (CK2alpha')

Plasmid

#27084

Depositor

David Litchfield

Article

Turowec et al Methods Enzymol. 2010;484:471-93.

Insert

CK2alpha' (CSNK2A2 Human)

Tags

GST

Expression

Bacterial

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pVSV eGFP RABV-G

Plasmid

#31833

Depositor

Connie Cepko

Article

Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

Insert

VSV genome

Expression

Bacterial

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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