30,743 results

1. pET28a-SpyTag002-MBP

   Plasmid

   #102831

   Purpose

   Bacterial expression of SpyTag002 fused to maltose binding protein

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyTag002-MBP
   

   Tags

   His6 and Thrombin cleavage site

   Expression

   Bacterial

   Mutation

   Peptide tag added to the N-terminus of MBP via a …

   Promoter

   T7

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKEW303-AcrVA5

   Plasmid

   #115657

   Purpose

   Expresses AcrVA5 in bacteria, under TetR control.

   Depositor

   Jennifer Doudna

   Article

   Watters et al Science. 2018 Oct 12;362(6411):236-239.

   Insert

   AcrVA5
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Ptet

   Available Since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET23b-Casp3-His

   Plasmid

   #11821

   Depositor

   Guy Salvesen

   Article

   Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

   Insert

   Caspase 3 (CASP3 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. ATX3-13Q

   Plasmid

   #184248

   Purpose

   Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)

   Depositor

   Sandra Macedo Ribeiro

   Article

   Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

   Insert

   ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Human, Synthetic)
   

   Tags

   6x His-Tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   C-terminal codon optimization for protein expres…

   Promoter

   T7 promoter

   Available Since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRIM3-rfp

   Plasmid

   #74073

   Purpose

   GentR version of pRIM2 carrying rfp

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   rfp
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   ptac

   Available Since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPhiC31o

   Plasmid

   #13794

   Purpose

   Expression of mouse codon-optimized PhiC31 recombinase

   Depositor

   Philippe Soriano

   Article

   Raymond et al PLoS One. 2007 Jan 17;2(1):e162. doi: 10

   Insert

   PhiC31o
   

   Expression

   Bacterial

   Available Since

   Jan. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. GFP-PIPK1 gamma 90

   Plasmid

   #22299

   Depositor

   Pietro De Camilli

   Article

   Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

   Insert

   human phosphatidylinositol phosphate kinase type 1 gamma 90 (PIP5K1C Human)
   

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPLICSFAST-PO-MT-P2A

   Plasmid

   #214899

   Purpose

   Expresses equimolar Peroxisome- and Mitochondria- targeted split-FAST fragments for Short-range PO-MT Contact sites

   Depositor

   Tito Calì

   Insert

   SPLICSFAST-PO-MT-P2A
   

   Tags

   none

   Expression

   Bacterial and Mammalian

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSWAP_Entry_H2_beta_CM

   Plasmid

   #184870

   Purpose

   pSwap plasmid part with H2_beta homology arms and chloramphenicol cassette

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Expression

   Bacterial

   Available Since

   March 2, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pColdI-GST-mGTSF1

    Plasmid

    #214671

    Purpose

    Bacterial expression of GST-tagged mouse GTSF1

    Depositor

    Phillip Zamore

    Article

    Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

    Insert

    GTSF1 (Gtsf1 Mouse)
    

    Tags

    6x His and GST

    Expression

    Bacterial

    Promoter

    CspA

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGal4x5-E4T-G210

    Plasmid

    #171077

    Purpose

    The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    Gal4-E4-Gless-cassette
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDest17_N-myc

    Plasmid

    #234045

    Purpose

    Bacterial expression of N-terminally 6His-tagged N-myc; includes TEV cleavage site

    Depositor

    Tanja Mittag

    Article

    Yang et al Nat Struct Mol Biol. 2024 Oct;31(10):156

    Insert

    N-myc (MYCN Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDF-RFP amber

    Plasmid

    #229524

    Purpose

    mCherry red fluorescent protein (RFP) reporter protein with an amber stop codon at position 13

    Depositor

    Thomas Huber

    Article

    Abdelkader et al Chem 102385

    Insert

    RFP amber
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pP4-C

    Plasmid

    #225097

    Purpose

    gfpUV reporter vector with a high performance adenine-dependent riboswitch

    Depositor

    Robert Batey

    Article

    Drogalis et al PLoS One. 2020 Dec 1;15(12):e0243155. do

    Insert

    synthetic riboswitch
    

    Expression

    Bacterial

    Promoter

    synthetic constitutive promoter

    Available Since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCN38-kimA-yfp

    Plasmid

    #227635

    Purpose

    c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding kimA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    kimA-yfp
    

    Expression

    Bacterial

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCN38-ktrA-yfp

    Plasmid

    #227636

    Purpose

    c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    ktrA-yfp
    

    Expression

    Bacterial

    Available Since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAM236 Ubc4

    Plasmid

    #226361

    Purpose

    Expression of yeast Ubc4

    Depositor

    Andreas Martin

    Article

    Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

    Insert

    Ubc4 (UBC4 Budding Yeast)
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Nov. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. Cut190*_pET21b

    Plasmid

    #215404

    Purpose

    Expression construct for Cut190* gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    Cut190*
    

    Expression

    Bacterial

    Mutation

    S226P/R228S

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28a-VcRibB

    Plasmid

    #187977

    Purpose

    Expresses 3,4-dihydroxy-2-butanone 4-phosphate synthase RibB from Vibrio cholerae

    Depositor

    Audrey Lamb

    Article

    Kenjic et al J Am Chem Soc. 2022 Jul 20;144(28):12769

    Insert

    VcRibB
    

    Tags

    Hexahistag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJBL3907

    Plasmid

    #193140

    Purpose

    Clostridium beijerinckii pfl ZTP riboswitch with sfGFP reporter

    Depositor

    Julius Lucks

    Article

    Bushhouse et al Nucleic Acids Res. 2023 Mar 2:gkad110. d

    Insert

    Clostridium beijerinckii (Cbe) pfl ZTP riboswitch with sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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