33,902 results
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Plasmid#181782PurposeExpresses ShHELIX containing a nicking I-AniI fusion to ShTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertnAniI-ShTnsB, ShTnsC, ShTniQ, ShCas12k
ExpressionBacterialMutationnAniI = K227M, F80K, L232KPromoterLac and J23119Available sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTU1-A-SP44-sfGFP
Plasmid#163757PurposeExpresses Streptomyces codon-optimised sfGFPDepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterSP44Available sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAT201
Plasmid#134878PurposeBacterial expressionDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable sinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-HA-AR-DBD
Plasmid#171238PurposeMammalian expression of 2xHA-tagged human androgen receptor trancate: AR DNA-bindind domain (DBD)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsert2xHA-tagged human androgen receptor trancate:DNA-binding domain (AR Human)
TagsHAExpressionBacterial and MammalianMutationAR truncatePromoterCMVAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLEVI(408)-ColE-iLight-msfGFP
Plasmid#170268PurposeExpresses LexA_DBD-iLight-msfGFP under J23116 promoter and mCherry under ColE promoter with a LexA408 operator in bacteria.DepositorInsertiLight
TagsmsfGFPExpressionBacterialMutationTo design iLight for expression in bacteria, a fu…PromoterpJ23116Available sinceJune 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pET-28a(+)::NL
Plasmid#141289PurposeNLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)DepositorInsertNLUC
UseLuciferaseExpressionBacterialMutationEngineered for high stability (t1/2 = 11.5 days a…Promoterpromoter for bacteriophage T7 RNA polymeraseAvailable sinceFeb. 17, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pM2s2TsR
Plasmid#137923PurposeE. coli Nissle pMUT2-derived plasmid with tetracycline resistance and constitutive RFP expressionDepositorInsertmRFP1
UseSynthetic Biology; For use with e. coli nissle in…ExpressionBacterialPromoterJ23101Available sinceJan. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-RT004-LaG30VB
Plasmid#163384Purpose2nd generation lentiviral vector to express anti-GFP(LaG30) module in the G-baToN systemDepositorInsertLaG30-VEEGFRtm-BFP (LaG30VB)
UseLentiviralTagsBFPExpressionBacterial and MammalianPromoterEF1aAvailable sinceJan. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET-iSeroSnFR
Plasmid#128483PurposeFluorescent reporter for serotonin (bacterial expression)DepositorInsertiSeroSnFR
TagsHis, T7, and cpsfGFPExpressionBacterialPromoterT7Available sinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSP1E2D1
Plasmid#161606PurposeBacterial expression of "extended" membrane scaffold protein MSP1E2D1 for Nanodisc formationDepositorInsertMSP1E2D1
Tags7-HisExpressionBacterialMutation"extended" MSP1D1; contains repeat of h…PromoterT7Available sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF
Plasmid#149446PurposeExpression of WT MIF in E. coli for protein productionDepositorAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPP089
Plasmid#158796PurposepRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-3 in MCS1 for expression in E. coli and protein purificationDepositorInsertCasPhi-3
ExpressionBacterialAvailable sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pD451-pA-APEX2
Plasmid#157863PurposeBacterial expression plasmid to express pA-APEX2 for antibody-based biotin proximity labelingDepositorInsertpA-APEX2
ExpressionBacterialAvailable sinceAug. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody
Plasmid#138128PurposeExpresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertmClover3-anti-mouse-IgG(kappa)-nanobody
TagsGSTExpressionBacterialAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody
Plasmid#138133PurposeExpresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.DepositorInsertEGFP-anti-rabbit-IgG(Fc)-nanobody
TagsGSTExpressionBacterialAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα13-LgB106
Plasmid#134364PurposeNanoluc complementation assay. Expression of Gα13 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 106 and 107 of Gα13. Addition of the HA epitope at N term of Gα13.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGα13-LgB106 (GNA13 Human)
TagsHAExpressionBacterial and MammalianPromoterT7Available sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCgCas9_recT
Plasmid#153337PurposeDouble-plasmid-based CRISPR-Cas9 system in Corynebacterium glutamicum; pBL1ts oriVC. glutamicum; pSC101 oriVE. coli PlacM-SpCas9 Streptomyces coelicolor codon-optimized, Peftu-RecT; KanrDepositorInsertCas9
UseCRISPRExpressionBacterialAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT-ADARcd-E488Q-V5
Plasmid#139686PurposeExpresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5DepositorAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHCL149
Plasmid#134457PurposeCmR, Para-popZ-rbs-H3H4-msfGFPN-CTM. Replicative plasmid for the expression of bait. N-terminal GFP fusion.DepositorInsertPara-popZ-rbs-H3H4-msfGFPN-CTM.
ExpressionBacterialPromoterParaAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only