30,416 results

1. Cas12aUltra_5XNLS

   Plasmid

   #218775

   Purpose

   His-TEV-Cas12aUltra-BPSV40_cMyc_NP_5NLS

   Depositor

   Ross Wilson

   Article

   Sahu et al bioRxiv 2024.07.14.603391

   Insert

   5xNLS-BPSV40-NLP-cMyc-cMyc-BPSV40- AspCas12a
   

   Use

   CRISPR

   Tags

   6XHis (C terminal), HA, Nucleoplasmin, SV40, and …

   Expression

   Bacterial

   Mutation

   Nucleotides mutations were made to encode for arg…

   Promoter

   Available Since

   Aug. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNCS-mClover3

   Plasmid

   #74236

   Purpose

   Constitutive expression of mClover3 in bacteria

   Depositor

   Michael Lin

   Article

   Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

   Has Service

   Cloning Grade DNA

   Insert

   mClover3
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-SUMO-hGSDMD

   Plasmid

   #111559

   Purpose

   Express human GSDMD in E.coli

   Depositor

   Hongbo Luo

   Article

   Kambara et al Cell Rep. 2018 Mar 13;22(11):2924-2936.

   Insert

   Gasdermin D (GSDMD Human)
   

   Use

   Tags

   His, SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETDuet-TFIIF

   Plasmid

   #171082

   Purpose

   Co-expresses human TFIIF. The resulting plasmid was used to generate a expression construct encoding 2 subunits, with His-tag on RAP30. Originally from MN Gonzalez, was submitted with permission

   Depositor

   Joan Conaway

   Article

   He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

   Inserts

   RAP30 (GTF2F2 Human)
   RAP74 (GTF2F1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pColdI-GST-mGTSF1

   Plasmid

   #214671

   Purpose

   Bacterial expression of GST-tagged mouse GTSF1

   Depositor

   Phillip Zamore

   Article

   Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

   Insert

   GTSF1 (Gtsf1 Mouse)
   

   Use

   Tags

   6x His and GST

   Expression

   Bacterial

   Mutation

   Promoter

   CspA

   Available Since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. VR122 pETv2 10His-pG-Tn5 (E54K, L372P)

   Plasmid

   #198467

   Purpose

   E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.

   Depositor

   Viviana Risca

   Article

   Soroczynski et al bioRxiv 2024.07.11.602973

   Insert

   10His-pG-Tn5 (E54K, L372P)
   

   Use

   Tags

   10His-pG

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   Consensus φ10 T7 Promoter

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGLO-GFP-1UAG

   Plasmid

   #82500

   Purpose

   Expresses GFP with 1 UAG codon at the amino acid position 3

   Depositor

   Chang Liu

   Article

   Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

   Insert

   Green Fluorescent Protein with 1 UAG codon
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Added an UAG codon at the amino acid position 3

   Promoter

   AraBAD

   Available Since

   Sept. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET28-Nsp14-TEV-Strep

   Plasmid

   #154953

   Purpose

   Expresses Nsp14 from SARS-CoV-2 with a C-terminal TEV-protease cleavable Strep tag in E. coli

   Depositor

   Justin Kollman

   Insert

   Nsp14-TEV-Strep (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   TEV-Strep

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

9. pHL 1405

   Plasmid

   #53010

   Purpose

   pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp

   Depositor

   Han Lim

   Article

   Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

   Inserts

   RhyB sRNA
   DsrA sRNA
   hfq
   sodB
   

   Use

   Synthetic Biology

   Tags

   gfp

   Expression

   Bacterial

   Mutation

   contains -56 to +141 relative to start codon

   Promoter

   pCon, pLlacO-1, and pLtetO-1

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pHL 1032

    Plasmid

    #52997

    Purpose

    pLtetO-1:RhyB, pCon:ompC::mCherry, pLlacO-1:sodB::gfp

    Depositor

    Han Lim

    Article

    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

    Inserts

    RhyB sRNA
    ompC
    sodB
    

    Use

    Synthetic Biology

    Tags

    gfp and mCherry

    Expression

    Bacterial

    Mutation

    contains −56 to +141 bp relative to start codon a…

    Promoter

    pCon, pLlacO-1, and pLtetO-1

    Available Since

    May 16, 2014

    Availability

    Academic Institutions and Nonprofits only