32,870 results
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Plasmid#157879PurposeEmpty vector backbone for defense systemsDepositorInsertNone
ExpressionBacterialAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG008 (Ec78 retron)
Plasmid#157886PurposeExpresses the Ec78 retron defense systemDepositorInsertEc78 retron
ExpressionBacterialAvailable SinceNov. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-A3A(N57Q)-BE3
Plasmid#158157PurposeT7 promoter bacterial expression plasmid for hAPOBEC3A(N57Q)-BE3 with N-terminal 6xHis-tag and C-terminal SV40 NLSDepositorInsert6xHis-hA3A(N57Q)-XTEN linker-hSpCas9n(D10A)-UGI-SV40NLS
Tags6xHis tag on N-terminusExpressionBacterialPromoterT7Available SinceNov. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSL1749 (pDonor, R*MmeI)
Plasmid#160733PurposeMini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.DepositorInsertVchCAST donor DNA
ExpressionBacterialPromoterN/AAvailable SinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC18/promEco/Lysostaphin
Plasmid#125765PurposeProduction of staphylolytic protein lysostaphin from a constitutive promoterDepositorInsertlysostaphin
ExpressionBacterialMutationsequence corresponding to the mature form proteinPromoterpemIK-Sa1 promoterAvailable SinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
R701-X65-527: His6-tev-Hs.CALM1(2-148)
Plasmid#159693PurposeE. coli protein expression of His6-tev-Hs.CALM1(2-148)DepositorAvailable SinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET11b-MIF
Plasmid#149446PurposeExpression of WT MIF in E. coli for protein productionDepositorAvailable SinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp16
Plasmid#156482PurposeBacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tagDepositorInsertNsp16 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1.Gα11-LgB97
Plasmid#134361PurposeNanoluc complementation assay. Expression of Gα11 protein fused with the LgBiT(LgB)of the nanoluciferase inserted between residues 97 and 98 of Gα11. Addition of the HA epitope at N terminus of Gα11.DepositorAvailable SinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4092677
Plasmid#153883PurposeBacterial expression plasmid for fragment SARS-CoV-2 orf1a stop to orf1b stopDepositorInsertfragment SARS-CoV-2 orf1a stop to orf1b stop (ORF1ab Severe acute respiratory syndrome coronavirus 2)
ExpressionBacterialMutationwt;handful of SNPs as watermarksPromoterPromoter | t7 consensus from NEB, G is +1Available SinceJune 12, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMAK464
Plasmid#140702PurposeNBαEGFPDepositorInsertNBαEGFP
ExpressionBacterialAvailable SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
[pSK131] pCOLADuet p14|MP1
Plasmid#136145Purposebacterial expression of p14 and MP1DepositorInsertp14 and MP1
ExpressionBacterialAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTW199b2
Plasmid#136928PurposeDirected evolution of SpCas9DepositorInsertgIII
UseCRISPRExpressionBacterialMutationnoneAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC-GFP-HS
Plasmid#133305Purpose"Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisationDepositorInserthok/sok toxin-antitoxin cassette
UseSynthetic BiologyExpressionBacterialAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T1 human PP2A B56α full length
Plasmid#132635Purposeexpresses human PP2A B56α full length in E. coliDepositorInsertPP2A B56α
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P-2-human RNF146 full length
Plasmid#132610Purposeexpresses human RNF146 full length in E. coliDepositorInsertRNF146 (RNF146 Human)
ExpressionBacterialAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)10
Plasmid#126948PurposeBacterial expression plasmid for (SUMO)10 client to assess SUMO-SIM scaffolds.DepositorInsert10 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
ExpressionBacterialPromoterunknownAvailable SinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTOX10
Plasmid#127455PurposeEncodes YhaV toxin. Gent-RDepositorInsertyhaV toxin
ExpressionBacterialPromoterpRhaAvailable SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBbB19k-GFP
Plasmid#126135PurposeConstitutive expression vector for Escherichia coli. BglBrick compatible.DepositorInsertGFPuv
UseSynthetic BiologyExpressionBacterialPromoterJ23105Available SinceJune 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG
Plasmid#124929PurposeExpression of RGG domain from LAF-1 geneDepositorAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only