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33,441 results

Showing: 3041 - 3060 of 33441 results

  4. pET-EIN-FTO(32-505)

    Plasmid
    #186466
    Purpose
    Expresses human RNA demythelase protein FTO 32-505 amino acid sequence with N-terminal fusion with the N-domain of EI ( e.coli protein)
    Depositor
    Vincenzo Venditti
    Publication
    Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055
    Insert
    Fat mass obesity associated protein (FTO Human)
    Tags
    6 Histidine-EIN (N-terminal domain of E.coli Enzy…
    Expression
    Bacterial
    Mutation
    amino acid 32-505
    Promoter
    T7
    Available since
    June 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    186466-plasmid-map-sequence-id-365741

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  5. SpyTag-sfGFP

    Plasmid
    #184226
    Purpose
    Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.
    Depositor
    Mark Howarth
    Publication
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    SpyTag-sfGFP
    Tags
    His6, SpyTag, and TEV protease cleavage site
    Expression
    Bacterial
    Promoter
    T5
    Available since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184226-plasmid-map-sequence-id-361605

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  8. pHN764

    Plasmid
    #178819
    Purpose
    Expression of EndoH in E. coli
    Depositor
    Kazuhiro Abe
    Insert
    synthetic gene encoding endo-β-N-acetylglucosaminidase H
    Tags
    HIs8 and MBP
    Expression
    Bacterial
    Promoter
    tac
    Available since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178819-plasmid-map-sequence-id-351934

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  11. pCE41

    Plasmid
    #174433
    Purpose
    C. auris LEUpOUT HYG marker gRNA expression construct. Use with pCE38 CAS9 expression construct.
    Depositor
    Clarissa Nobile
    Publication
    Ennis et al Microbiol Spectr. 2021 Dec 22;9(3):e0182
    Insert
    HYG 2 of 2, pSNR52, ADE2 gRNA, gRNA conserved, C. auris LEU2 2 of 2
    Use
    CRISPR
    Expression
    Bacterial and Yeast
    Available since
    Dec. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    174433-plasmid-map-sequence-id-349717

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  13. pMB1_1R26PylRS(CbzK)_AfTyrRS(p-I-Phe)_AlvtRNA-ΔNPyl(8)(CGA)_AftRNA-Tyr(A01)(CUA)

    Plasmid
    #174516
    Purpose
    Recoded double aaRS/tRNA plasmid with 1R26PylRS/AlvtRNA-ΔNPyl(8)(CGA) & AfTyrRS/AftRNA-Tyr(A01)(CUA) to incorporate CbzK into the TCG codon & p-I-Phe into the TAG codon.
    Depositor
    Jason W Chin
    Publication
    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.
    Inserts
    PylS_1R26 - CBZK
    Af_TyrRS - p-I-Phe
    Ma tRNA pylT(8) with CGA anticodon
    A.fulgidus tRNA with CUA anticodon
    Expression
    Bacterial
    Mutation
    CUA anticodon, M.alvus Pyl tRNA with CGA anticodo…
    Promoter
    GlnS and lpp
    Available since
    Oct. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    174516-plasmid-map-sequence-id-343328

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  16. pKAR2-Br512g3.1

    Plasmid
    #171200
    Purpose
    Expresses Br512g3.1 polymerase in E.coli
    Depositor
    Andrew Ellington
    Publication
    Bhadra et al medRxiv 2022.08.09.22278605
    Insert
    Engineered Bst DNA Polymerase I Large Fragment
    Tags
    8x His tag; Villin Headpiece 47 amino acid (vHP47…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    171200-plasmid-map-sequence-id-339557

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Showing: 3041 - 3060 of 33441 results