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32,387 results

Showing: 3041 - 3060 of 32387 results

  6. pConRef-2H12

    Plasmid
    #221182
    Purpose
    cdGreen expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11213)
    Depositor
    Urs Jenal
    Article
    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:
    Inserts
    cdGreen
    mScarlet-I
    Expression
    Bacterial
    Mutation
    N-terminus changed: starts with MSKKYGEAVIKE ...
    Promoter
    Constitutive and None (same as cdGreen)
    Available Since
    June 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    221182-plasmid-map-sequence-id-437177

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  8. pAC-crRNA-Km

    Plasmid
    #158712
    Purpose
    Plasmids containing the medium-copy-number p15A origin of replication can be propagated in E. coli cells and a non-targeting crRNA
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    AcGFP (for crRNA cloning)
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Promoter
    TetO
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    158712-plasmid-map-sequence-id-321925

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  11. KEAP1 (KEAP1A-c029)

    Plasmid
    #110247
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KEAP1 (KEAP1 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    110247-plasmid-map-sequence-id-212283

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  12. pTF-ahpC-rfp

    Plasmid
    #153035
    Purpose
    For CRISPR-Cas12a genome editing in E. coli. Contains a constituitively expressed CRISPR array targeting the ahpC gene in E. coli and donor DNA to insert an rfp translational fusion in the ahpC gene.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Expression
    Bacterial
    Available Since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153035-plasmid-map-sequence-id-318838

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  14. pHelper-CBE-sgRNA

    Plasmid
    #221137
    Purpose
    Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with Cas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221137-plasmid-map-sequence-id-436076

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  18. pACYC-T7-SpCas9-T7-EGFP_gRNA1-T7term (BPK848)

    Plasmid
    #181745
    Purpose
    dual T7 promoter vector for bacterial expression of SpCas9 with a c-terminal NLS and 3xFLAG tag, along with an SpCas9 sgRNA targeting EGFP site 1
    Depositor
    Benjamin Kleinstiver
    Article
    Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038
    Insert
    human/zebrafish codon optimized SpCas9
    Use
    In vitro transcription; t7 promoter
    Tags
    NLS(SV40)-3xFLAG
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Feb. 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    181745-plasmid-map-sequence-id-354676

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  19. Dox-Akt

    Plasmid
    #207328
    Purpose
    PiggyBac vector backbone encoding doxycycline-inducible expression of membrane-localized Akt1 (without pleckstrin homology domain)
    Depositor
    Jared Toettcher
    Article
    Underhill et al Development. 2023 Aug 15;150(16):dev2016
    Insert
    rac protein kinase-alpha (AKT1 Human)
    Tags
    myristoylation tag - BFP
    Expression
    Bacterial
    Mutation
    range: 129 - 480
    Promoter
    Tet Response Element
    Available Since
    Nov. 2, 2023
    Availability
    Academic Institutions and Nonprofits only
    207328-plasmid-map-sequence-id-413074

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  20. mCherry VPS37b

    Plasmid
    #245030
    Purpose
    Protein localization of human wild-type VPS37b in cells
    Depositor
    Thomas Martin
    Insert
    VPS37b (VPS37B Human)
    Tags
    mCherry
    Expression
    Bacterial
    Available Since
    Sept. 30, 2025
    Availability
    Academic Institutions and Nonprofits only
    245030-plasmid-map-sequence-id-487497

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Showing: 3041 - 3060 of 32387 results