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pDest_PANCEd_AraC_R2_S170_LOV_P2.1_C3

Plasmid

#242155

Purpose

Expression Plasmid containing a LacI repressed sd2 AraC R2-LOV POGO-PANCE P2.1.3 variant cassette

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Insert

AraC R2 S170 LOV P2.1.3

Expression

Bacterial

Mutation

AraC: E3K, E144K, R146L, A152T, S225R, R251L; AsL…

Available Since

Nov. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pLIC-His6-MBP-TEV-Rgs14

Plasmid

#236395

Purpose

Expresses His6-MBP-TEV-Rgs14 in E. coli

Depositor

John Hepler

Article

Brown et al J Biol Chem. 2015 Apr 3;290(14):9037-49.

Insert

Rattus norvegicus regulator of G-protein signaling 14 (Rgs14 Rat)

Tags

hexahistidine (H6) tag and maltose-binding protei…

Expression

Bacterial

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pRExT2A-CT-BmSc

Plasmid

#232208

Purpose

Endogenous tagging of genes by CRISPR/Cas9-mediated homologous recombination; for C-terminal tagging: mScarlet-3xTy-T2A-BSD-3xTy

Depositor

Calvin Tiengwe

Article

Carbajo et al Open Biol. 2025 Feb;15(2):240334. doi: 1

Insert

mScarlet

Expression

Bacterial

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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Pxyl-ftsZ-sfGFP

Plasmid

#174505

Purpose

Integrated plasmid harboring ftsZ-sfGFP gene, inducible by xylose (sequence of sfGFP optimized)

Depositor

Suliana Manley

Article

Mahecic et al Nat Methods. 2022 Sep 8. pii: 10.1038/s4

Insert

ftsZ

Tags

superfold GFP

Expression

Bacterial

Available Since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003 S49C

Plasmid

#133448

Purpose

Expresses SpyCatcher003 S49C in bacterial cytoplasm, with a unique cysteine for chemical coupling

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003 S49C

Tags

GSSGS, His6, and TEV cleavage site

Expression

Bacterial

Mutation

Contains S49C mutation

Promoter

T7

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pBBR1MCS-2

Plasmid

#85168

Purpose

Mobilisable shuttle and expression vector. Replicates in many Gram-negative bacteria. Has multiple cloning site with blue/white selection function. Cloned genes driven by derepressed lac promoter.

Depositor

Kenneth Peterson

Article

Kovach et al Gene. 1995 Dec 1;166(1):175-6.

Has Service

Cloning Grade DNA

Type

Empty backbone

Expression

Bacterial

Promoter

Constitutive (derepressed P-lac)

Available Since

Nov. 8, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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6xHis-SNAP-PI(4)P Probe

Plasmid

#211509

Purpose

For expression of recombinant biosensor for detection of Phosphatidylinositol-4-Phosphate (PI(4)P) from bacteria

Depositor

Hannes Maib, David Murray

Article

Maib et al J Cell Biol. 2024 Jun 3;223(6):e20231009

Insert

SidC (P4C)

Tags

6xhis-SNAP

Expression

Bacterial

Available Since

Jan. 19, 2024

Availability

Academic Institutions and Nonprofits only

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prhaBAD-anti-mouse-Nb-Hia5

Plasmid

#239626

Purpose

Rhamnose inducible expression plasmid for anti-mouse nanobody fusion to Hia5 DNA adenine methyltransferase

Depositor

Aaron Straight

Article

Gamarra et al bioRxiv [Preprint]. 2025 Mar 14:2025.03.

Insert

Hia5

Tags

6HIS, MBP, TEV, and anti-Mouse Nanobody

Expression

Bacterial

Mutation

Codon optimized for bacterial expression

Promoter

rhaBAD

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a_6H-TAQ_E602D

Plasmid

#166944

Purpose

Bacterial expression of Taq DNA polymerase

Depositor

Robert Tjian

Article

Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

Insert

Thermus aquaticus DNA polymerase

Tags

6X His

Expression

Bacterial

Mutation

E602D

Promoter

T7

Available Since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Phusion

Plasmid

#206210

Purpose

Inducible expression of DNA polymerase

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2018 Aug 15;13(8):e0201681. do

Insert

Phusion DNA polymerase

Expression

Bacterial

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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