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pGI3EM17

Plasmid

#210569

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

SpH2Bpro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Mutation

Promoter

SpH2Bpro

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pEK22

Plasmid

#210588

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

BdH3pro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Mutation

Promoter

BdH3pro

Available since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pDusk

Plasmid

#43795

Purpose

Empty backbone for blue-light-regulated expression of a gene of interest. Expression is repressed under blue illumination.

Depositor

Andreas Moeglich

Article

Ohlendorf et al J Mol Biol. 2012 Mar 2;416(4):534-42. do

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pBS-iNOS

Plasmid

#19295

Depositor

Charles Lowenstein

Article

Lowenstein et al Proc Natl Acad Sci U S A. 1992 Aug 1. 89

Insert

inducible nitric oxide synthase (Nos2 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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pYUB28b

Plasmid

#37277

Depositor

Ted Baker

Article

Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

Type

Empty backbone

Use

Mycobacteria shuttle vector

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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pD441_WT_hi

Plasmid

#128857

Purpose

High copy number plasmid for expression of E. coli flagellin from an IPTG-inducible T5 promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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KLHL40 (KBTBD5A-c006)

Plasmid

#98233

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

KBTBD5A (KLHL40 Human)

Use

Tags

TEV-His6-Flag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-SENP2 (catalytic domain)

Plasmid

#16357

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

human SENP2 catalytic domain (SENP2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Amino Acid 365 to 590 catalytic domain of human S…

Promoter

Available since

Dec. 19, 2007

Availability

Academic Institutions and Nonprofits only

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pPSU15

Plasmid

#173828

Purpose

Expresses 15 kD Penn State ladder protein

Depositor

Song Tan

Article

Santilli et al Sci Rep. 2021 Aug 18;11(1):16703. doi: 1

Insert

HSTPABPAC

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLG008 (Ec78 retron)

Plasmid

#157886

Purpose

Expresses the Ec78 retron defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Ec78 retron

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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hUbc13

Plasmid

#51131

Purpose

human ubc13, E2 for synthesis k63 linked ubiquitin chain

Depositor

Cynthia Wolberger

Article

Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

Insert

ubc13 (UBE2N Human)

Use

Tags

His6 and SUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 29, 2015

Availability

Academic Institutions and Nonprofits only
pFNF

Plasmid

#22687

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

Neo*

Use

Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

FRT-pPGK-pEM7-Neo*-bpA-FRT

Promoter

Available since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-v2 RsTAL At4CL

Plasmid

#126524

Purpose

expresses TAL (Rhodobacter sphaeroides) and 4CL (Arabidopsis thaliana paralog 1) for PYP chromophore biosynthesis

Depositor

Andrew Woolley

Article

Brechun et al Biochemistry. 2019 Jun 11;58(23):2682-26

Inserts

tyrosine ammonia lyase
p-coumaroyl-CoA ligase (4CL1 Mustard Weed)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pBbA6c_PsulA_sfGFP

Plasmid

#224587

Purpose

SOS response reporter

Depositor

Ophelia Venturelli

Article

Cheng et al Mol Syst Biol. 2023 Mar 9;19(3):e11406.

Insert

sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PsulA

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_EV-D68_VP1

Plasmid

#202072

Purpose

Bacterial expression of VP1 protein from enterovirus D68 (EV-D68). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

EV-D68 VP1 protein with Histag

Use

Tags

6xHis-tag and GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pGR618

Plasmid

#213786

Purpose

Integrates at the CAN1 locus the BB-Tv TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette

Depositor

Chang Liu

Insert

BB-Tv TP-DNAP1

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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pTE100

Plasmid

#59395

Purpose

MethyloBrick cloning vector, no promoter; TcR

Depositor

Tobias Erb

Article

Schada von Borzyskowski et al ACS Synth Biol. 2014 Aug 8.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

none

Available since

Oct. 23, 2014

Availability

Academic Institutions and Nonprofits only

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pSC101-GFPmut2-mScarlet-I

Plasmid

#208183

Purpose

mistranslation GFP and mScarlet-I positive control

Depositor

Tanel Tenson

Article

Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10

Inserts

GFPmut2
mScarlet-I

Use

Reporter

Tags

Expression

Bacterial

Mutation

Promoter

Ptet+dnaK P1

Available since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-2xMreBN-mCherry-His

Plasmid

#133626

Purpose

Expression vector for mCherry fused to a tandem repeat of the E. coli MreB membrane targeting sequence (MTS) (N-terminal fusion) and a C-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

2xMreB-mCherry-His

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET28-MHL: nsp10_SARS-CoV-2

Plasmid

#167851

Purpose

Bacterial expression of the SARS-CoV-2 protein nsp10

Depositor

Cheryl Arrowsmith

Article

Perveen et al SLAS Discov. 2021 Jan 10:247255522098504

Insert

nsp10

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 16, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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