32,395 results

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pPIPL12-FnCas12a

Plasmid

#199563

Purpose

Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia

Depositor

Philippe Lambin

Article

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

Fncas12a

Use

CRISPR and Synthetic Biology; Tetracycline induci…

Expression

Bacterial

Promoter

COtetR-PIPL12

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pPfetFn-Target_v2

Plasmid

#199564

Purpose

gRNA expression for FnCas12a in E. coli and clostridia

Depositor

Philippe Lambin

Article

Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

Insert

SacB

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

Pthl_fU

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE160

Plasmid

#118508

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

TagBFP2

Expression

Bacterial

Available Since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pTcI39-switch

Plasmid

#195557

Purpose

Temperature state switch expressing low-temperature mWasabi and high-temperature mRFP1

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Inserts

mRFP1
mWasabi
TcI39

Tags

6x His and AANDENYADAS degradation tag

Expression

Bacterial

Mutation

A67T

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pMIIS638

Plasmid

#238210

Purpose

Expresses native SpTasH TIGR locus. CmR

Depositor

Feng Zhang

Article

Faure et al Science. 2025 Feb 27:eadv9789. doi: 10.1

Insert

SpTasH

Expression

Bacterial

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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CBla_2.0_EmrE_TMD4_3P

Plasmid

#207848

Purpose

BLaTM-System; Antiparallel positive control EmrE_TMD4_3P in CBLa 2.0 vector

Depositor

Dieter Langosch

Article

Julius et al J Mol Biol. 2017 Apr 18. pii: S0022-2836

Insert

C-BLa 2.0 fusion protein

Tags

Flag (DYKDHDG)

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pOPINB-HOIL-1 full-length (1–510)

Plasmid

#193858

Purpose

Expression of human His-tagged HOIL-1 (RBCK1) full-length (1–510) codon optimized for E. coli

Depositor

Bernhard Lechtenberg

Article

Wang et al Nat Commun. 2023 Jan 11;14(1):168. doi:

Insert

HOIL-1 (RBCK1 Human)

Tags

3C protease cleavage site and 6x-His tag

Expression

Bacterial

Mutation

Codon optimised for expression in E. coli

Promoter

T7

Available Since

Jan. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pUCP30T-eCFP

Plasmid

#78476

Purpose

Broad range bacterial fluorescence labeling (ECFP)

Depositor

Mariette Barbier

Article

Barbier et al PLoS One. 2016 Mar 3;11(3):e0146827. doi

Insert

eCFP

Expression

Bacterial

Available Since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCDF-His-mTET1CD

Plasmid

#81053

Purpose

bacterial expression of the catalytic domain of murine TET1

Depositor

Primo Schaer

Article

Weber et al Nat Commun. 2016 Mar 2;7:10806. doi: 10.

Insert

TET1 (Tet1 Mouse)

Tags

6HIS

Expression

Bacterial

Mutation

catalytic domain amino acid 1367-2057

Promoter

T7

Available Since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pQTEV- PRDX2

Plasmid

#31338

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

PRDX2 (PRDX2 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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SpyTag-β-Lactamase-SpyCatcher

Plasmid

#52656

Purpose

Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.

Depositor

Mark Howarth

Article

Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2

Insert

TEM-1 β-Lactamase

Tags

SpyCatcher and SpyTag

Expression

Bacterial

Promoter

T7 promoter

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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pBbS5a-OptoCre-REDMAP

Plasmid

#228465

Purpose

Light-inducible Cre recombinase split with REDMAP photoreceptors for bacterial expression

Depositor

Mary Dunlop

Article

Jafarbeglou et al ACS Synth Biol. 2024 Nov 19. doi: 10.102

Insert

OptoCre-REDMAP

Use

Cre/Lox

Expression

Bacterial

Promoter

pLacUV5

Available Since

March 12, 2025

Availability

Academic Institutions and Nonprofits only

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pSR1038

Plasmid

#208737

Purpose

Characterization plasmid for PCap (sfgfp expressed from PCap on the S. aureus pC194 replicon)

Depositor

Lauren Andrews

Article

Rondthaler et al ACS Synth Biol. 2023 Dec 8. doi: 10.1021

Insert

PCap

Use

Synthetic Biology

Expression

Bacterial

Promoter

PCap

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_TP53BP2-1/1

Plasmid

#91503

Purpose

Protein expression and purification of human SH3 domain construct TP53BP2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

TP53BP2-1/1 (TP53BP2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTlpA36-Wasabi

Plasmid

#86115

Purpose

mWasabi reporter gene gated by TlpA36

Depositor

Mikhail Shapiro

Article

Piraner et al Nat Chem Biol. 2017 Jan;13(1):75-80. doi

Inserts

TlpA36
mWasabi

Tags

6xHis

Expression

Bacterial

Mutation

D341A, P60L, D135V, K187R, K202I, L208Q

Promoter

pTlpA and pTlpA, LacI

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pMT85_SpdCas9_pmcDA1

Plasmid

#204614

Purpose

CRISPR cytosine deaminase integrative plasmid for Mycoplasma bovis

Depositor

Pascal Sirand-Pugnet

Article

Ipoutcha et al Appl Environ Microbiol. 2022 Sep 13;88(1

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pMRE163

Plasmid

#118511

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Article

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sYFP2

Expression

Bacterial

Available Since

Aug. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-Glt1-eGFP

Plasmid

#176862

Purpose

Expresses Glt-1 fused with GFP at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Glt1-eGFP

Use

AAV

Tags

GFP

Expression

Bacterial

Promoter

gfaABC1D

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SpdCas9-LBiT_Strep-Tag-II

Plasmid

#198751

Purpose

Expresses S. Pyogenes dCas9 fused to LargeBiT fragment of split NanoLuc luciferase

Depositor

Maarten Merkx

Article

van der Veer et al ACS Cent Sci. 2023 Mar 15;9(4):657-667.

Insert

SpdCas9 - LargeBiT

Tags

Strep-tag II

Expression

Bacterial

Mutation

D10A, H840A (nuclease inactivating mutations in &…

Promoter

T7

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-N2H-N1

Plasmid

#125547

Purpose

Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1

Depositor

Marc Vidal

Article

Choi et al Nat Commun. 2019 Aug 29;10(1):3907. doi:

Type

Empty backbone

Use

In vitro expression

Tags

NanoLuc fragment1 attached to n-terminus of Gatew…

Expression

Bacterial and Mammalian

Promoter

synthetic promoter containing yeast, mammalian, a…

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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