32,529 results
-
Plasmid#18668PurposepET19b converted Gateway Destination vector for expressing His tagged fusion proteins in bacteria. See note in comments section below regarding the mutated enterokinase site.DepositorTypeEmpty backboneUseGateway CloningTagsHisExpressionBacterialPromoterT7Available SinceJuly 26, 2008AvailabilityAcademic Institutions and Nonprofits only
-
RanBP2-RB3-4-pET23a
Plasmid#85769Purposebacterial expression of RanBP2 SUMO E3 ligase fragment (aa 2304-3062) with C-terminal His tagDepositorInsertRanBP2 fragment (RANBP2 Human)
TagsHisExpressionBacterialMutationfragment (amino acids 2304-3062)PromoterT7Available SinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-Trx-mSA2
Plasmid#52320PurposeSolubly expresses monomeric streptavidin containing S25H mutation in E. coli as a thioredoxin fusion. App Microbiol & Biotech 98, 6285-6295 (2014)DepositorInsertmonomeric streptavidin 2
TagsFLAG, His, S tag, TEV site, and ThioredoxinExpressionBacterialMutationS25H mutation compared to streptavidinPromoterT7Available SinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
mKate1.31-pBAD
Plasmid#54827PurposeLocalization: Protein Expression Vector , Excitation: 588, Emission: 635. Also known as mKate2.DepositorTypeEmpty backboneTags6xHis and mKate1.31 (aka mKate2)ExpressionBacterialAvailable SinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pComb3XTT
Plasmid#63891PurposeBackbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)DepositorInsertTT Fab
UsePhage displayTags6xHis, HA tag, and geneIIIExpressionBacterialPromoterlacZAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMVV052
Plasmid#215303PurposeExpresses sfGFP under rhamnose induction (RhaS + RhaR + pRhaB-sfGFP) with a BBR1 backbone.DepositorInsertsfGFP
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterPRhaBAvailable SinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p1-GST-ArcFL
Plasmid#119877PurposeBacterial Expression construct for protein production and purificationDepositorAvailable SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
OmpA3-PNGaseF-TEV-His6
Plasmid#114274PurposeAmidase removing almost all N-linked oligosaccharides from glycoproteins. This PNGase F construct can have its hexa-his removed.DepositorInsertPeptide: N-glycosidase F
TagsOmpA3 leader sequence (for periplasmic localisati…ExpressionBacterialPromoterT7Available SinceAug. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pmRFP_LAP2beta_IRES_puro2b
Plasmid#21047DepositorAvailable SinceJuly 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCR2.1-InC
Plasmid#83959PurposeContains a synthetic insert to serve as internal control DNA template for a Salmonella Real-Time PCR assay.DepositorInsertInternal Control sequence
ExpressionBacterialAvailable SinceNov. 2, 2016AvailabilityAcademic Institutions and Nonprofits only