30,424 results

1. pGGF_RT_FAST_RFP

   Plasmid

   #136979

   Purpose

   Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

   Depositor

   Klaus Theres

   Article

   Rossmann et al Plant J. 2019 Oct 30. doi: 10.1111/tpj.1

   Insert

   seed coat marker RFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

   Promoter

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHCL151

   Plasmid

   #134455

   Purpose

   TetR, lacI-Plac-ssdsbA-mscarN, Integrative plasmid for the expression of fully-screted prey. N-terminal mScarlet-I fusion.

   Depositor

   Thomas Bernhardt

   Article

   Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

   Insert

   linker-mScarlet-I for C-terminal fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   plac

   Available Since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAD-GamS

   Plasmid

   #99246

   Purpose

   Expresses 6xHis-tagged GamS from T7lac promoter in the E.coli Tuner (DE3) strain. See notes below for important strain information.

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   gamS (with C-terminal 6xHis tag)
   

   Use

   Tags

   6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTKEI-Tre-C04

   Plasmid

   #79754

   Purpose

   Single fluorescent protein biosensor for trehalose

   Depositor

   David Savage

   Article

   Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

   Insert

   Tre-C04
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   pTrc

   Available Since

   July 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pdCas9

   Plasmid

   #46569

   Purpose

   Expresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.

   Depositor

   Luciano Marraffini

   Article

   Bikard et al Nucleic Acids Res. 2013 Jun 12.

   Inserts

   tracrRNA
   dcas9
   CRISPR array
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A, H840A

   Promoter

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHCL147

   Plasmid

   #134454

   Purpose

   TetR, lacI-Plac-ssdsbA-mscarC, Integrative plasmid for the expression of fully-secreted prey. C-terminal mScarlet-I fusion.

   Depositor

   Thomas Bernhardt

   Article

   Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111

   Insert

   linker-mScarlet-I for C-terminal fusion
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   plac

   Available Since

   Jan. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETM6-G6-vioABE-4A6-vioC-3A2-vioD

   Plasmid

   #73440

   Purpose

   Violacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.

   Depositor

   Mattheos Koffas

   Article

   Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

   Insert

   PG6-vioABE-P4A6-vioC-P3A2-vioD
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PG6, P4A6, and P3A2 (orthogonal T7-lac variants)

   Available Since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pORTMAGE-Pa1

   Plasmid

   #138475

   Purpose

   Enables high-efficiency recombineering in P. aeruginosa

   Depositor

   George Church

   Article

   Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

   Inserts

   PapRecT
   mutL E36K
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E36K

   Promoter

   Available Since

   June 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDXinit

   Plasmid

   #110101

   Purpose

   E.coli entry and expression vector for FX cloning system, N-terminal pelB signal sequence and C-terminal fusion to PIII for phage display using M13 phages

   Depositor

   Markus Seeger

   Article

   Zimmermann et al Elife. 2018 May 24;7. pii: 34317. doi: 1

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBFC0984

    Plasmid

    #231992

    Purpose

    CRISPRi-ART plasmid encoding aTc-inducible dRfxCas13d and constitutive crRNA with 2xBsaI spacer cloning site

    Depositor

    Brady Cress

    Article

    Adler et al Nat Microbiol. 2025 Mar;10(3):694-709. d

    Inserts

    dRfxCas13d
    crRNA with 2xBsaI spacer
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119 and pTet

    Available Since

    April 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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