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32,401 results

Showing: 3841 - 3860 of 32401 results

  4. pCas12f1

    Plasmid
    #171182
    Purpose
    Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1
    Depositor
    Kohsuke Honda
    Article
    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124
    Insert
    Cas12f1
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    cas9 promoter from Streptococcus pyogenes
    Available Since
    June 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    171182-plasmid-map-sequence-id-336407

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  9. pBRC901

    Plasmid
    #167948
    Purpose
    express wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
    Depositor
    James Naismith
    Article
    Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:
    Insert
    wild-type bacterial tyrosine kinase Wzc from E. coli E69 (O9a:K30)
    Tags
    hexa-histidine tag
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    Oct. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    167948-plasmid-map-sequence-id-344794

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  10. pVH015

    Plasmid
    #80398
    Purpose
    Contains pBAD-CusR (response regulator) and pCusR-antiscaffold-GFP(3xFLAG). Used for Western blots
    Depositor
    Richard Murray
    Article
    Hsiao et al ACS Synth Biol. 2015 Feb 20;4(2):150-61.
    Inserts
    Anti-scaffold-ffGFP-3xFLAG
    CusR (cusR )
    AraC (araC )
    Use
    Synthetic Biology
    Tags
    C-terminal 3xFLAG tag and C-terminal LZx domain
    Expression
    Bacterial
    Available Since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    80398-plasmid-map-sequence-id-155896

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  15. pIA123

    Plasmid
    #234038
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.
    Depositor
    Craig Ellermeier
    Article
    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Pgdh and Pxyl
    Available Since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    234038-plasmid-map-sequence-id-465702

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  18. TTC5

    Plasmid
    #39064
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    TTC5 (TTC5 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  20. pET-29b(+)_His-CBD-SpEndoH

    Plasmid
    #218196
    Purpose
    Bacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoH
    Depositor
    Florence Pojer
    Insert
    EndoH
    Tags
    tufA-His-CBD
    Expression
    Bacterial
    Mutation
    codon optimized Ecoli, residues 45-313
    Promoter
    T7
    Available Since
    April 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    218196-plasmid-map-sequence-id-431799

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Showing: 3841 - 3860 of 32401 results