-
Plasmid#159376PurposeExpresses gSTEP1 in E. coliDepositorInsertgSTEP1
UseTags6x His tagExpressionBacterialMutationPromoterT7Available sinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_USP7_MATH
Plasmid#178490PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertUSP7_MATH (USP7 Synthetic, Human)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-143
Plasmid#118527PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertsYFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH6HTC_minus_cer-region
Plasmid#175297PurposeProduction of HaloTag fusion proteinDepositorTypeEmpty backboneUseCell free expressionTagsHaloTag-6xHisExpressionBacterialMutationPromoterAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a Gx-mNG-NL
Plasmid#169106PurposeExpresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.DepositorInsertprotein G - mNeonGreen - NanoLuc
UseTagshis-tag and strep-tagExpressionBacterialMutationAla 24 stopPromoterT7Available sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEDJ333
Plasmid#177272PurposeGAL1p:Cas9:CYC1tDepositorInsertCas9
UseTagsExpressionBacterial and YeastMutationPromoterGAL1Available sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
BPV00500 pET21GG2 His(6)-TF-BECN(1-450)
Plasmid#136684PurposeExpresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor)DepositorInsertBeclin-1 (BECN1 Human)
UseTagsTrigger FactorExpressionBacterialMutationDNA optimized for E. coli expressionPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pL0M-S-mTurquoise2-EC18152
Plasmid#137074PurposeGolden Gate Level 0 S module for N-terminal fluorescent protein taggingDepositorInsertmTurquoise2 DNA synthesis product with 5' and 3' extensions for Golden Gate cloning
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJune 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBBR8k-GFPuv
Plasmid#106385PurposeL-arabinose-inducible araBAD promoter expressing gfpuv on BBR1 originDepositorInsertGFPuv
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMarch 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSP1E3D1
Plasmid#199772PurposeExpresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)DepositorInsertcMSP1E3D1
UseTagsHis6-TagExpressionBacterialMutationExtended cMSP1D1; external His-Tags flanking the …PromoterAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
nGCaMP2-NpuDnaEn
Plasmid#34850DepositorInsertnGCaMP2-NpuDnaEn
UseTagsExpressionBacterial and MammalianMutationPromoterCMV, T7 lacAvailable sinceMarch 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET19-MTdTwt
Plasmid#207460PurposeBacterial expression of Terminal deoxynucleotidyl transferase (TdT)DepositorInsertMTdTwt (Dntt Mouse)
UseTags10xHis-MBPExpressionBacterialMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBAD_sfGFP3TCG4TAG
Plasmid#174518PurposeRecoded sfGFP reporter with the codons TCG and TAG inserted at positions 3 and 4, respectively.DepositorInsertsfGFP
UseTags6 x HisExpressionBacterialMutationRecoded. TCG and TAG codons were introduced in po…PromoteraraBADAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1TFAKRS
Plasmid#177311PurposetRNA synthetase/tRNA pair for the in vivo incorporation of N6-(trifluoroacetyl)-L-lysine (TFA-Lys) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJOE_pvdJ
Plasmid#110142PurposeSuicide plasmid with repair template for knockout in P. putidaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp6-DARPinOFF7
Plasmid#182398PurposeBacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusionDepositorInsertVNp6-DARPinOFF7
UseTagsVesicle Nucleating peptide 6 (VNp6)ExpressionBacterialMutationPromoterT7Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAJM.884
Plasmid#108536PurposeAcuRAM + PAcu-YFP Reporter. Acr sensor for use in non-Marionette strain.DepositorInsertAcuRAM + PAcu-YFP
UseTagsExpressionBacterialMutationAcuR: E80K R154H L202MPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
HeLa full-length MLCK-GFP
Plasmid#46316DepositorInsertHeLa long myosin light chain kinase (MYLK Human)
UseTagsGFPExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
IL2 pMalLP
Plasmid#41808DepositorInsertIL2 (IL2 Human)
UseTags3C protease site, 6x His, and Maltose Binding Pro…ExpressionBacterialMutationPromoterpTacAvailable sinceJan. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(4CNW)RS
Plasmid#222871PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 4-Cyano-L-Tryptophan (4-CN-Trp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only