29,754 results

1. pOPINO_NbH11-H4

   Plasmid

   #155364

   Purpose

   Expresses nanobody H11-H4, which binds to the receptor binding domain (RBD) of the spike protein of SARS-Cov-2

   Depositor

   Ray Owens

   Article

   Huo et al Nat Struct Mol Biol. 2020 Jul 13. pii: 1

   Insert

   H11-H4
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pEMF037

   Plasmid

   #157634

   Purpose

   Batis maritima MHT

   Depositor

   Jonathan Silberg

   Article

   Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

   Insert

   MHT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pScaf-3024.1

   Plasmid

   #111404

   Purpose

   pScaf phagemid with insert for generating DNA origami scaffold 3024 bases in length

   Depositor

   Shawn Douglas

   Article

   Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

   Insert

   pScaf-3024
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 16, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-His-SCov2-nsp8

   Plasmid

   #154758

   Purpose

   Expression of SARS-CoV-2 nsp8

   Depositor

   Patrick Cramer

   Article

   Hillen et al Nature. 2020 May 21. pii: 10.1038/s41586

   Insert

   SARS-CoV-2 nsp8 (ORF1ab Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSB233

   Plasmid

   #164128

   Purpose

   PP7-CFP and PhlF-RFP expression plasmid

   Depositor

   Christopher Voigt

   Article

   Shao et al Nat Commun. 2021 Mar 5;12(1):1475. doi:

   Insert

   PP7-CFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptet

   Available since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX-Pak1 83-149

   Plasmid

   #12216

   Depositor

   Jonathan Chernoff

   Article

   Xiao et al J Biol Chem. 2002 Jan 11. 277(2):883-6.

   Insert

   Pak1 (Pak1 Rat)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Dominant negative Pak1: autoinhibitory domain, aa…

   Promoter

   Available since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

7. PDLIM4_PDZ_1

   Plasmid

   #103941

   Purpose

   Protein expression and purification of RIL BGLII PDZ domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PDLIM4 (PDLIM4 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBR322-Pdp1-HiBiT

   Plasmid

   #198310

   Purpose

   Loads HiBiT-tagged Pdp1 into PVCs; for payload loading assays; also contains Pnf

   Depositor

   Feng Zhang

   Article

   Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

   Insert

   Pdp1-HiBiT
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTetO-ISce1-TetO

   Plasmid

   #58506

   Purpose

   ISce1 restriction site is flanked by Tet operator repeats, 96 on each side

   Depositor

   Tom Misteli

   Article

   Roukos et al Science. 2013 Aug 9;341(6146):660-4. doi

   Insert

   Tet repeats flanking ISce1 restriction site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   no promoter

   Available since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pACYC-T7-Nla-repeat-BbsI

    Plasmid

    #180215

    Purpose

    For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

    Depositor

    Yan Zhang

    Article

    Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pIBA2-Int-SpyTag

    Plasmid

    #198038

    Purpose

    Expression plasmid for producing SpyTagged stalled secretion variant of intimin

    Depositor

    Jack Leo

    Article

    Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

    Insert

    eaeA
    

    Use

    Tags

    SpyTag

    Expression

    Bacterial

    Mutation

    lacks native signal peptide (amino acids 1-39)

    Promoter

    tet

    Available since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMBEC8

    Plasmid

    #187599

    Purpose

    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Apramycin resistance

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

    Insert

    nCas9-BEC-UGI; cas6f, gfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    trc

    Available since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. SpyCatcher002-oDi

    Plasmid

    #124661

    Purpose

    Expresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coil

    Depositor

    Mark Howarth

    Article

    Khairil Anuar et al Nat Commun. 2019 Apr 15;10(1):1734. doi:

    Insert

    SpyCatcher002-oDi
    

    Use

    Tags

    C-tag, His6, and TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAtetO_6xHis-Klentaq

    Plasmid

    #157862

    Purpose

    Plasmid for the aTc-inducible expression of the Klentaq

    Depositor

    Andrew Ellington

    Article

    Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

    Insert

    Klentaq
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    tet PA

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRK2-AraE

    Plasmid

    #110141

    Purpose

    Constitutive promoter expressing araE on RK2 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    araE
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJJS001

    Plasmid

    #188324

    Purpose

    PCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.

    Depositor

    Mike Boxem

    Article

    Sepers et al G3 (Bethesda). 2022 Aug 27. pii: 6677660

    Insert

    mIAA7::GFP::Linker
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. p406CYC1

    Plasmid

    #15975

    Depositor

    Fred Cross, Nicolas Buchler

    Insert

    CYC1 promoter + polylinker + CYC1 terminator (CYC1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    CYC1 promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. DCX-RFP

    Plasmid

    #32851

    Depositor

    Joseph Gleeson

    Article

    Tanaka et al J Cell Biol. 2004 Jun 7. 165(5):709-21.

    Insert

    DCX (DCX Human)
    

    Use

    Tags

    DsRed2

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 15, 2011

    Availability

    Academic Institutions and Nonprofits only

19. human p53-(311-393)

    Plasmid

    #24869

    Depositor

    Cheryl Arrowsmith

    Article

    Ayed et al Nat Struct Biol. 2001 Sep . 8(9):756-60.

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Contains aa 311-393.

    Promoter

    Available since

    Aug. 5, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pGEX-hXRCC1

    Plasmid

    #52274

    Purpose

    Bacterial expression of human XRCC1 with N-terminal GST-tag

    Depositor

    Primo Schaer

    Article

    Weber et al PLoS One. 2014 Jul 9;9(7):e102157. doi:

    Insert

    XRCC1 (XRCC1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    July 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge