30,753 results

1. pTYB1-T4uvsX

   Plasmid

   #228213

   Purpose

   Expression in BL21(DE3) of T4 recombinase

   Depositor

   Stephen Benkovic

   Insert

   uvsX (uvsX Escherichia phage T4)
   

   Tags

   Sce VMA intein-chitin binding domain

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pExodus CMV.Artemis

   Plasmid

   #40211

   Purpose

   Mammalian expression of codon-optimized Artemis.

   Depositor

   Andrew Scharenberg

   Article

   Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

   Insert

   Artemis (DCLRE1C Human)
   

   Expression

   Bacterial and Mammalian

   Promoter

   CMV and T7

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. bdSUMO-pHD118-GFP-HiBit

   Plasmid

   #171015

   Purpose

   For bacterial expression of bdSUMO tagged muGFP with pHD118 peptide and NanoLuc complementation peptide variant #86

   Depositor

   Angus Johnston

   Article

   Teo et al Nat Commun. 2021 Jun 17;12(1):3721. doi:

   Insert

   14x-His-bdSUMO-pHD118-muGFP-HiBiT
   

   Use

   Luciferase

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDule-3-nitroTyrosine (5B)

   Plasmid

   #85498

   Purpose

   Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

   Insert

   3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32H H70C D158S I159A L162R

   Promoter

   lpp (constitutive)

   Available Since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-Matr3 delRRM1

   Plasmid

   #32881

   Depositor

   Yossi Shiloh

   Article

   Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

   Insert

   MATR3 Matrin3 (MATR3 Human)
   

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   deleted amino acids 399-468

   Promoter

   CMV

   Available Since

   Jan. 31, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)

   Plasmid

   #48285

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTn7-SCOUT12.G

   Plasmid

   #201000

   Purpose

   pLv1-pUC18R6KT-miniTn7-Km-sfGFP, KmR, ApR

   Depositor

   Philip Poole

   Article

   Jorrin et al Microbiome. 2024 May 7;12(1):81. doi: 10

   Insert

   superfolder GFP
   

   Expression

   Bacterial

   Promoter

   J23104

   Available Since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZS*12-MS2-GFP

   Plasmid

   #26245

   Depositor

   Philippe Cluzel

   Article

   Guet et al Nucleic Acids Res. 2008 Jul . 36(12):e73

   Insert

   MS2-GFP
   

   Expression

   Bacterial

   Mutation

   five amino acid linker (Ser, Gly, Gly, Gly, Gly) …

   Available Since

   Nov. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEX-6P1-Vinculin tail

   Plasmid

   #107154

   Purpose

   bacterial expression

   Depositor

   Tina Izard

   Article

   Chinthalapudi et al J Cell Biol. 2014 Dec 8;207(5):643-56. d

   Insert

   Vinculin (VCL Human)
   

   Tags

   GST

   Expression

   Bacterial

   Promoter

   Ptac

   Available Since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX2-Cas9

    Plasmid

    #85811

    Purpose

    Arabinose inducible Cas9 in a broad host range backbone.

    Depositor

    Ryan Gill

    Insert

    Cas9
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSR59.4

    Plasmid

    #63175

    Purpose

    Expresses OmpR constitutively from PompB97 and sfGFP under the PompF146 promoter

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

    Inserts

    OmpR (ompR )
    sfGFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. 560HTR

    Plasmid

    #24444

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Tags

    6XHis

    Expression

    Bacterial

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pMRE-Tn7-141

    Plasmid

    #118557

    Purpose

    miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mTurquoise2
    

    Use

    Minitn7 delivery vector

    Expression

    Bacterial

    Available Since

    Oct. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBad33_ISAam1_reRNA

    Plasmid

    #205322

    Purpose

    Bacterial expression of ISAam1 TnpB and its reRNA

    Depositor

    Haoyi Wang

    Article

    Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

    Insert

    ISAam1 TnpB
    

    Expression

    Bacterial

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. RPO41

    Plasmid

    #205072

    Purpose

    RPO41 protein expression

    Depositor

    Smita Patel

    Article

    Tang et al J Biol Chem. 2009 Feb 27;284(9):5514-22.

    Insert

    RPO41 (RPO41 Budding Yeast)
    

    Expression

    Bacterial

    Mutation

    Lacks N Terminal 100 amino acids

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET-X(4130)B1

    Plasmid

    #168018

    Purpose

    Expresses X(4130)B1-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Blum et al Science. 2021 Feb 19;371(6531):803-810.

    Insert

    X(4130)B1-6xHis
    

    Tags

    Hisx6

    Expression

    Bacterial

    Available Since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. WT C-His Ana GvpC in pET28a

    Plasmid

    #85732

    Purpose

    Expresses His-tagged GvpC from Anabaena flos-aquae in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al ACS Nano. 2016 Aug 23;10(8):7314-22. doi

    Insert

    Gas vesicle protein C (his-tagged)
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Added an extra glycine after the methionine at th…

    Promoter

    T7

    Available Since

    March 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSF4 TetCMV 5'TOP intron 20xGCN4 Renilla FKBP Stop 24xMS2v5 SV40 CTE polyA

    Plasmid

    #119946

    Purpose

    Expresses 5'TOP-SunTag-Renilla mRNA reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    Renilla luciferase
    

    Tags

    24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

    Expression

    Bacterial and Mammalian

    Promoter

    TetCMV

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. MDH2

    Plasmid

    #38792

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    MDH2 (MDH2 Human)
    

    Tags

    His Tev

    Expression

    Bacterial

    Available Since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

20. pDIV296

    Plasmid

    #204687

    Purpose

    AMA1 plasmid with nat1 resistance marker

    Depositor

    Uffe Mortensen

    Article

    Vanegas et al J Fungi (Basel). 2022 Dec 22;9(1):16. do

    Insert

    nat1
    

    Use

    Fungal expression

    Expression

    Bacterial

    Promoter

    Aspergillus nidulans trpC promoter

    Available Since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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