33,449 results
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Plasmid#198296PurposeProduces PVCs retargeted with HA tagDepositorInsertPVCpnf_pvc13-HA
ExpressionBacterialAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
SSB-wt
Plasmid#197260Purposewild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistanceDepositorInsertwt E. coli SSB
TagsnoneExpressionBacterialPromoterT7Available sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
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EB3-mNeonGreen
Plasmid#191761PurposeExpression of mNeonGreen fused to EB3DepositorInsertEB3-mNeonGreen (MAPRE3 Human)
TagsmNeonGreenExpressionBacterial and MammalianPromoterCMVAvailable sinceFeb. 8, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pReg-RamR
Plasmid#190610PurposeExpresses the RamR transcription factor in E. coliDepositorInsertRamR
ExpressionBacterialAvailable sinceJan. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCWU6-xylR-E-luciferase
Plasmid#190721Purposefine tune expression of luciferase under the control of xylose- and theophylline- inducible systemDepositorInsertxylR-E-luciferase
UseLuciferaseExpressionBacterialAvailable sinceNov. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPROEX-TwStrp-FTO
Plasmid#190265PurposeExpresses FTO in bacterial cells, with two purification tags: His and Twin-Streptag.DepositorAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHPSE-P6
Plasmid#185907PurposeEngineered Human Heparanase mutant for e.coli expressionDepositorTagsHis-tagExpressionBacterialPromoterT7Available sinceAug. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDae029 (eMutaT7[TadA-8e])
Plasmid#187620PurposeExpresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coliDepositorInsertTadA-8e-T7RNAP
ExpressionBacterialAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHybrid-Electra1
Plasmid#179474PurposeExpresses Electra1 in bacterial cells and Electra1 and mScarlet in mammalian cellsDepositorInsertsElectra1
mScarlet-I
ExpressionBacterial and MammalianAvailable sinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMBEC6
Plasmid#187598PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Gentamycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
ExpressionBacterialPromotertrcAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMBEC2
Plasmid#187596PurposeBase editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistanceDepositorInsertnCas9-BEC-UGI; cas6f, gfp
ExpressionBacterialPromotertrcAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYTK-DN2
Plasmid#180283PurposeSpacer cassette (TU3)DepositorInsertConL2 - Spacer - ConRE
ExpressionBacterialAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGJJ045
Plasmid#183621PurposeabundancePCA plasmid - Contains a barcode landing pad and two fragments of the murine methotrexate-resistant DHFR, the expression of one driven by the CYC promoter and the other by the GPD promoter.DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a Halotag-CNA35
Plasmid#131128PurposeHalotag-CNA35 peptide for Collagen labellingDepositorInsertHalotag-CNA35
TagsHalotagExpressionBacterialAvailable sinceMay 10, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWM_12x601_45bpLinker
Plasmid#157791PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_45bp linker
ExpressionBacterialAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJA5 H-2K(b)
Plasmid#180441PurposeE. coli expression of soluble MHC proteinDepositorInsertH-2K(b)
TagsBSP41ExpressionBacterialMutationReplacement of transmembrane domain with enzymati…Available sinceMarch 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7FTrp)RS
Plasmid#177310PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Fluoro-L-Tryptophan (7FTrp) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
ExpressionBacterialPromoterT7Available sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T3-GST-EGFP
Plasmid#177136PurposeBacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFPDepositorInsertEGFP
ExpressionBacterialPromoterTacAvailable sinceDec. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
p2B-58
Plasmid#177928PurposeExpresses yeGFP under control of doxycycline-responsive TET4 promoter (contains four rtTA binding sites)DepositorPublicationInsertEGFP
ExpressionBacterial and YeastPromoterTET4 (contains four rtTA binding sites)Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only