33,128 results
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Plasmid#149249PurposeBacterial expression plasmid for SARS-CoV-2 3C-like proteinaseDepositorInsertSARS-CoV-2 3C-like proteinase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 11, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046799
Plasmid#145577PurposeBacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymeraseDepositorInsertSARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFP.R267
Plasmid#138179PurposeExpresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHimar6
Plasmid#137081Purposebacterial Himar1 mini-transposon donor plasmidDepositorInsertHimar1 mini-transposon with chloramphenicol resistance gene
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
HS69 pComb3xss anti-Homer1 nanobody
Plasmid#134716PurposeBacterial expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-Homer1 (Mouse) recombinant llama nanobody (Homer1 L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM5406
Plasmid#132661PurposeRSF1010-based broad host-range plasmid with additional RK2 bom site and pUC originDepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)10-(SIM)5
Plasmid#126866PurposeBacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.DepositorInsert10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
ExpressionBacterialPromotertacAvailable SinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH25-SceI
Plasmid#129389PurposeDerived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.DepositorInsertChloramphenicol acetyltransferase
ExpressionBacterialMutationN/APromoterCAT promoterAvailable SinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRDV5 containing Sb-convex
Plasmid#132696PurposepRDV5 plasmid containing a convex Sybody for the generation of a qPCR standard curveDepositorInsertSb_convex including 5' and 3' flanking sequences used for ribosome display
UseSynthetic BiologyExpressionBacterialPromoterT7Available SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH610
Plasmid#131862PurposeProduces holo-PhyB* in E. coliDepositorInsertsPhyB(1-651)-RGD-His6-Cys
HO1
PcyA
ExpressionBacterialPromoterT7 promoter-1 and T7 promoter-2Available SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETDest
Plasmid#124245PurposeAllows Gateway cloning into the bacterial 6xHIS tagged protein expression vector pET26DepositorTypeEmpty backboneExpressionBacterialAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAC1430
Plasmid#127981PurposeAMA1 plasmid with Aspergillus optimized Cpf1, pyrG selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable SinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPEPX-Plac
Plasmid#122632PurposeBglFusion compatible IPTG inducible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPEPY
Plasmid#122633PurposeBglFusion compatible integration vector for Streptococcus pneumoniaeDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceAug. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-His6-Sumo-CfaC-MBP
Plasmid#126018PurposeBacterial expression of CfaC-MBP fusion protein (with N-terminal His6-Sumo)DepositorInsertHis6-Sumo-CfaC-MBP
Tags6xHis-SUMO and HA-MBPExpressionBacterialAvailable SinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT13-splitD
Plasmid#122597Purposeclone M13 phage by SapI Golden GateDepositorInsertM13 bacteriophage genes
ExpressionBacterialAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAH01Sp::Cas9
Plasmid#128160PurposePtet-controlled expression of S. pyogenes Cas9DepositorInsertSp Cas9
ExpressionBacterialPromoterPtetAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAH01
Plasmid#128162PurposeIncP-based broad host range vector used for inducible expression of genes in methanotrophic bacteriaDepositorTypeEmpty backboneExpressionBacterialPromoterPtetAvailable SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet1-Cdc45
Plasmid#126878PurposeExpresses human Cdc45 in bacteriaDepositorAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET_RGG-RGG-RGG
Plasmid#124938PurposeExpression of RGG-RGG-RGGDepositorAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only