30,756 results

1. pSP64T Activin betaB (DM#108)

   Plasmid

   #15045

   Depositor

   Douglas Melton

   Article

   Thomsen et al Cell. 1990 Nov 2. 63(3):485-93.

   Insert

   Activin Beta b (inhbb Frog)
   

   Expression

   Bacterial

   Available Since

   Oct. 18, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBbA2k-RFP

   Plasmid

   #35327

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Tet

   Available Since

   Feb. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJM230 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD-stRBS-lacZ)

   Plasmid

   #110560

   Purpose

   miniTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoter and StRBS-lacZ insert

   Depositor

   Joanna Goldberg

   Article

   Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

   Insert

   stRBS-lacZ
   

   Expression

   Bacterial

   Promoter

   rhaSR-PrhaBAD

   Available Since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. p324_DG

   Plasmid

   #234690

   Purpose

   Golden gate domesticated pET28b with TEV-His6

   Depositor

   Matthew Wohlever

   Article

   Gaur et al Protein Sci. 2025 Apr;34(4):e70106. doi:

   Type

   Empty backbone

   Tags

   TEV-His6

   Expression

   Bacterial

   Mutation

   No

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEVA221TadA*-T7RNAP

   Plasmid

   #167980

   Purpose

   Expresses fusion protein TadA*-T7RNAP in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

   Insert

   TadA*-T7RNAP
   

   Tags

   T7 tag and T7RNAP

   Expression

   Bacterial

   Promoter

   TetR-Ptet

   Available Since

   June 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRMC2-sec:msfGFP

   Plasmid

   #194914

   Purpose

   Tetracycline inducible expression of msfGFP fused to Sec signal peptide in Staphylococci

   Depositor

   Rikke Meyer

   Article

   Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

   Insert

   Shine Dalgarno sequence followed by Sec signal peptide fused to monomeric superfolder GFP
   

   Use

   Tetracycline-inducible expression vector for stap…

   Expression

   Bacterial

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. nuc-mCherry-pTRIP

   Plasmid

   #163520

   Purpose

   Used to construct lentivirus to express nucleus-targeted mCherry in mammalian cells

   Depositor

   Thomas Weber

   Insert

   nucleus targeted mCherry
   

   Use

   Lentiviral

   Expression

   Bacterial and Mammalian

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMycoFos

   Plasmid

   #84577

   Purpose

   Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium. Copy number is controllable by arabinose in E.coli.

   Depositor

   Nicholas Coleman

   Article

   Ly et al J Microbiol Methods. 2011 Sep;86(3):320-

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 7, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pGMCtKq28-TSC10M1-sspB

   Plasmid

   #49526

   Purpose

   Kanamycin resistant; contains qtag28, expresses sspB under a promoter containing PtetO, and constutively expresses single-chain TetR10 (tsc10); integrates into the mycobacteriophage att-Tweety site

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   TSC10M1-sspB
   

   Expression

   Bacterial

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGMCK-T38S38-750-luxAB-DAS+4

    Plasmid

    #49518

    Purpose

    Kanamycin resistant; expresses luxAB-DAS+4 under the control of a promoter containing PtetO-4C5G; constitutively expresses reverse TetR (tetR38); integrates into the mycobacteriophage att-L5 site

    Depositor

    Dirk Schnappinger

    Article

    Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

    Insert

    T38S8-P750-luxAB-DAS+4
    

    Tags

    DAS+4 (AANDENYSENYADAS)

    Expression

    Bacterial

    Promoter

    P750

    Available Since

    Jan. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-Tag4A-MITF-M (wt)

    Plasmid

    #31151

    Depositor

    Yardena Samuels

    Article

    Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

    Insert

    MITF-M (MITF Human)
    

    Tags

    FLAG

    Expression

    Bacterial and Mammalian

    Mutation

    WT

    Available Since

    July 22, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAT217

    Plasmid

    #134885

    Purpose

    Bacterial expression

    Depositor

    Thomas Howard

    Article

    Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

    Insert

    mRFP1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    July 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCoofy1-psHaloTag1a

    Plasmid

    #232940

    Purpose

    Photoswichable HaloTag: psHaloTag1a variant

    Depositor

    Claire Deo

    Article

    Walterspiel et al bioRxiv 2024.12.18.629107

    Insert

    photoswitchable HaloTag 1a variant
    

    Tags

    His6x-HRV3C

    Expression

    Bacterial

    Mutation

    E143W, A258W with linkers L1 (FAG) and L2 (P)

    Promoter

    T7

    Available Since

    March 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-SUMO-DogTag

    Plasmid

    #105629

    Purpose

    Plasmid for recombinant expression in bacteria of SUMO domain fused to DogTag, permitting covalent linkage to SnoopTagJr

    Depositor

    Mark Howarth

    Article

    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

    Insert

    SUMO-DogTag
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET His6 NusA TEV LIC cloning vector (2N-T)

    Plasmid

    #29709

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6-NusA-TEV

    Expression

    Bacterial

    Available Since

    Aug. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28b_RanWT

    Plasmid

    #186270

    Purpose

    Expression vector of His-RanQ69L in E.coli

    Depositor

    Masahito Ikawa

    Article

    Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

    Insert

    GTP-binding nuclear protein Ran (Ran Mouse)
    

    Tags

    His tagged

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSWEET-bgaB

    Plasmid

    #210272

    Purpose

    Xylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.

    Depositor

    Eric Brown

    Article

    Bhavsar et al Appl Environ Microbiol. 2001 Jan;67(1):4

    Insert

    bgaB
    

    Expression

    Bacterial

    Promoter

    PxylA

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. psgRNAv1-empty

    Plasmid

    #171611

    Purpose

    sgRNA_v1 plasmid for AsCas12f1 in bacteria

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1_sgRNA_1
    

    Expression

    Bacterial

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGST-DHFR Mm

    Plasmid

    #83073

    Purpose

    Expresses the full length DHFR Mm in E.coli

    Depositor

    Gali Prag

    Article

    Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

    Insert

    2x DHFR (Dhfr Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Nov. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMBEC2

    Plasmid

    #187596

    Purpose

    Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistance

    Depositor

    Pablo Ivan Nikel

    Article

    Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

    Insert

    nCas9-BEC-UGI; cas6f, gfp
    

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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