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pHS1_10xHis_MBP_TEV_WT-Cas9

Plasmid

#244825

Purpose

Bacterial expression of SpyCas9 (WT) for protein purification

Depositor

Jennifer Doudna

Article

Shi et al Mol Cell. 2025 May 1;85(9):1730-1742.e9.

Insert

SpyCas9 (WT)

Use

CRISPR

Tags

10xHis-MBP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAD-mKate(KPS12)

Plasmid

#133831

Purpose

Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides.

Depositor

Oscar Kuipers

Article

Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

Insert

mKate(KPS12)

Use

E.coli/bacillus spp. shuttle vector

Expression

Bacterial

Promoter

Pupp

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a(His10-PD-1cyto)

Plasmid

#177896

Purpose

E coli expression of His10 tag fused to human PD-1 Intracellular Domain

Depositor

Ron Vale

Article

Hui et al Science. 2017 Mar 31;355(6332):1428-1433

Insert

PD-1 (aa 194-288 only) (PDCD1 Human)

Tags

10xHis-Thrombin cut site-T7 tag

Expression

Bacterial

Promoter

T7

Available Since

May 18, 2024

Availability

Academic Institutions and Nonprofits only

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pTXTL-T7max-NanoLuc

Plasmid

#188313

Purpose

Expression of NanoLuc via a T7 promoter in TXTL

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

NanoLuc

Use

Luciferase and Synthetic Biology

Tags

8x His-tag

Expression

Bacterial

Promoter

T7Max

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAM236 Ubc4

Plasmid

#226361

Purpose

Expression of yeast Ubc4

Depositor

Andreas Martin

Article

Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

Insert

Ubc4 (UBC4 Budding Yeast)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pCAS9counter

Plasmid

#107192

Purpose

Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

Depositor

Steve Salipante

Article

Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

Inserts

Cas9
sgRNA

Expression

Bacterial

Promoter

prab17

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pKT-CNP

Plasmid

#211803

Purpose

Expresses 2',3'-cyclic nucleotide phosphodiesterase catalytic domain

Depositor

Emily Weinert

Article

Fontaine et al Biochem J. 2018 Apr 30;475(8):1491-1506.

Insert

2',3'-cyclic nucleotide phosphodiesterase catalytic domain (Cnp Rat)

Expression

Bacterial

Promoter

tet

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pWSK129-stm2585

Plasmid

#174401

Purpose

S. Typhimurium stm2585 (sarA/steE) flanked with endogenous regulatory sequences (786bp on 5' and 28bp on 3' end) for bacterial expression.

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

sarA

Expression

Bacterial

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pSWEET-bgaB

Plasmid

#210272

Purpose

Xylose-dependent expression system for tight and modulated expression of cloned genes in Bacillus subtilis.

Depositor

Eric Brown

Article

Bhavsar et al Appl Environ Microbiol. 2001 Jan;67(1):4

Insert

bgaB

Expression

Bacterial

Promoter

PxylA

Available Since

March 14, 2024

Availability

Academic Institutions and Nonprofits only

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ILF2_DZF_pGEX

Plasmid

#135162

Purpose

Recombinant expression of ILF2 with dual-affinity tag

Depositor

Christopher Burge

Article

Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

Insert

ILF2 (ILF2 Human)

Tags

GST-SBP

Expression

Bacterial

Promoter

tac

Available Since

Nov. 14, 2020

Availability

Academic Institutions and Nonprofits only

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