30,535 results

1. pCDFDuet-1 MKK4(EE)-MKK7a1(EE)

   Plasmid

   #47580

   Purpose

   Bacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Inserts

   MKK4-EE (MAP2K4 Human)
   MKK7a1-EE (Map2k7 Mouse)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Lacks the first 36 residues of the RefSeq protein…

   Promoter

   Available Since

   Sept. 19, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pET26b_Nb.b201

   Plasmid

   #131404

   Purpose

   E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

   Depositor

   Andrew Kruse

   Article

   McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

   Insert

   Nanobody b201
   

   Use

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-ATG8(416)/GFP-AUT7(416)

   Plasmid

   #49425

   Purpose

   Expresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.

   Depositor

   Daniel Klionsky

   Article

   Guan et al Mol Biol Cell. 2001 Dec;12(12):3821-38.

   Insert

   autophagy-related 8 (ATG8 Budding Yeast)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   ATG8

   Available Since

   Jan. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28c-F30-2xdBroccoli

   Plasmid

   #66843

   Purpose

   Expresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cells

   Depositor

   Samie Jaffrey

   Article

   Filonov et al Curr Protoc Chem Biol. 2016 Mar 16;8(1):

   Insert

   Two dimeric Broccoli aptamers in the F30 scaffold
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. mt POLRMT

   Plasmid

   #205059

   Purpose

   POLRMT protein expression

   Depositor

   Smita Patel

   Article

   Ramachandran et al Nucleic Acids Res. 2017 Jan 25;45(2):861

   Insert

   POLRMT (POLRMT Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized

   Promoter

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET-B-Sniper SpCas9-NLS-6xHis

   Plasmid

   #207377

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-Sniper SpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   F539S, M763I, K890N, amino acids 1005-1013 replac…

   Promoter

   T7

   Available Since

   Sept. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUCSh5vio-2300

   Plasmid

   #170987

   Purpose

   Encodes sgRNA target 2300 and Gentamicin resistance - violacein pathway transposon.

   Depositor

   Christopher Reisch

   Article

   Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

   Insert

   Sh-CAST sgRNA 2300 violacein
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   placI

   Available Since

   July 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pST39

   Plasmid

   #64009

   Purpose

   original polycistronic cloning vector for plasmid suite (generation I)

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)

   Plasmid

   #140082

   Purpose

   Entry cloning vector for in vitro transcription or expression of SpCas9 sgRNAs from a T7 promoter

   Depositor

   Keith Joung

   Insert

   pT7-[BsaI_cassette]-SpCas9_sgRNA_scaffold (MSP3485)
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQE30-His-IF1

    Plasmid

    #124123

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Takuya Ueda, Sebastian Maerkl

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Initiation factor 1 (infA Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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