30,806 results

1. pNR239

   Plasmid

   #79576

   Purpose

   A118 attB-attP. GFP will express if sites recombine.

   Depositor

   Timothy Lu

   Article

   Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

   Insert

   GFPmut3b
   

   Expression

   Bacterial

   Available Since

   July 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMUT2-pFF-41.5-mCherry

   Plasmid

   #192863

   Purpose

   pMUT2 based vector for sensing oxygen in E. coli Nissle 1917

   Depositor

   Morten Sommer

   Article

   Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

   Insert

   Oxygen inducible promoter
   

   Expression

   Bacterial

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPGN-K

   Plasmid

   #174579

   Purpose

   pOPIN-GG expression vector compatible with N-terminal tagging, Kanamycin resistant

   Depositor

   Mark Banfield

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Oct. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZE27MC3

   Plasmid

   #75464

   Purpose

   Plasmid for constitutive expression of mcherry-pdt3

   Depositor

   James Collins

   Article

   Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

   Insert

   mcherry-pdt#3
   

   Expression

   Bacterial

   Promoter

   placIq promoter

   Available Since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pOPINK

   Plasmid

   #41143

   Depositor

   Ray Owens

   Insert

   GST
   

   Expression

   Bacterial

   Available Since

   March 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDLxR6

   Plasmid

   #172560

   Purpose

   Inducible gene expression, pCDF origin, LuxR activator, PLuxB promoter, mRFP, Streptomycin Resistance Marker.

   Depositor

   Christopher Reisch

   Insert

   LuxR-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLuxB

   Available Since

   Nov. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDFNKI-strepII-3C-LIC-strep

   Plasmid

   #108708

   Purpose

   Expression of a StrepII-tagged target protein with 3C protease cleavage site.

   Depositor

   Titia Sixma

   Article

   Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

   Type

   Empty backbone

   Tags

   StrepII-3C protease cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBEST-OR2-OR1-Pr-UTR1-deGFP-T500

   Plasmid

   #40019

   Depositor

   Vincent Noireaux

   Article

   Shin et al J Biol Eng. 2010 Jun 24;4:8.

   Insert

   deGFP
   

   Expression

   Bacterial

   Promoter

   OR2-OR1-Pr (bacteriophage Lambda with one mutatio…

   Available Since

   Oct. 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCWori-A13AMO-aaCPRct

   Plasmid

   #20117

   Depositor

   Jay Keasling

   Article

   Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

   Insert

   Amorphadiene oxidase and p450 reductase
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Feb. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL3287

    Plasmid

    #139750

    Purpose

    Base Cas12a CRISPRi plasmid

    Depositor

    Himadri Pakrasi

    Article

    Knoot et al ACS Synth Biol. 2020 Jan 17;9(1):132-143

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET15b-ACPs (from S. pneumonia)

    Plasmid

    #63687

    Purpose

    Inducible expression of Acyl Carrier Protein Synthase (ACPs) from S. pneumoniae

    Depositor

    Michael Johnson

    Insert

    acpS
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

12. pRSET-pr-Kaede (Kaede-A69T)

    Plasmid

    #99228

    Purpose

    Bacterial expression of the primed conversion capable protein pr-Kaede (Kaede-A69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-Kaede
    

    Expression

    Bacterial

    Mutation

    A69T for primed conversion applications

    Promoter

    T7

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pST44-AP-1 core

    Plasmid

    #184809

    Purpose

    Bacterial expression of clathrin adaptor AP-1 core complex

    Depositor

    James Hurley

    Article

    Ren et al Cell. 2013 Feb 14;152(4):755-67. doi: 10

    Insert

    Hs AP1S3; Hs AP1B1 (1-584); Mm AP1G1 (1-595); Mm AP1M1
    

    Tags

    GST tag and His6

    Expression

    Bacterial

    Mutation

    AP1S3: S148C; AP1B1: K359R, E476K

    Promoter

    T7

    Available Since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEND-227_Cacnes_mCerulean

    Plasmid

    #225614

    Purpose

    Replicative vector for blue fluorescent reporter protein mCerulean strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+mCerulean.

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Insert

    mCerulean
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for Cutibacterium acnes

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMRBAD-link-CGFP

    Plasmid

    #52733

    Purpose

    L-(+)-arabinose-inducible expression of link-CGFP negative control for in vivo split GFP assembly assay

    Depositor

    Lynne Regan

    Article

    Magliery et al J Am Chem Soc. 2005 Jan 12;127(1):146-57

    Insert

    link-CGFP
    

    Expression

    Bacterial

    Promoter

    PBAD

    Available Since

    May 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pH6HTC_TKT-Halo

    Plasmid

    #175331

    Purpose

    Production of HaloTag fusion protein

    Depositor

    Claudia Kutter

    Article

    Atanasoai et al bioRxiv 2021.11.08.467743

    Insert

    TKT (TKT Human)
    

    Use

    Cell free expression

    Tags

    HaloTag-6xHis

    Expression

    Bacterial

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. 6920 pPROEXHsp104b

    Plasmid

    #1230

    Depositor

    Susan Lindquist

    Article

    Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

    Insert

    Hsp104 (HSP104 Budding Yeast)
    

    Tags

    his tag (tev cleavable)

    Expression

    Bacterial

    Available Since

    Aug. 15, 2005

    Availability

    Academic Institutions and Nonprofits only

18. pYFJ16-LplA(W37V) (for E. Coli expression)

    Plasmid

    #34838

    Depositor

    Alice Ting

    Article

    Uttamapinant et al Proc Natl Acad Sci U S A. 2010 Jun 15. 1

    Insert

    LplA W37V (lplA E. Coli)
    

    Tags

    His Tag

    Expression

    Bacterial

    Mutation

    Mutated Trp37 to Val

    Promoter

    T5 promoter

    Available Since

    May 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSFDuet-1_VNp-mNeongreen-DARPinOFF7

    Plasmid

    #182415

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen-DARPinOFF7 fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-mNeongreen-DARPinOFF7
    

    Tags

    Vesicle Nucleating peptide (VNp) & mNeongreen

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28_human-Caspase-4_enzymatic-domain

    Plasmid

    #183381

    Purpose

    Overexpression of catalytic domain of inflammatory caspase in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-4 (AA94-377) (CASP4 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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