30,535 results

1. pUC18T-mini-Tn7T-hph-Ptet1

   Plasmid

   #218367

   Purpose

   Empty backbone for Tn7-site insertion with tetR-PtetA expression system with single tetR operator site

   Depositor

   Michael Gebhardt

   Article

   Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX SHIP1-SH2

   Plasmid

   #46492

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHIP1 (INPP5D Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-118

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-29b(+)_His-CBD-3C-EmPNGaseF

   Plasmid

   #218193

   Purpose

   Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola PNGaseF

   Depositor

   Florence Pojer

   Insert

   PNGaseF
   

   Use

   Tags

   tufA-His-CBD-3C

   Expression

   Bacterial

   Mutation

   codon optimized Ecoli, residues 41-354

   Promoter

   T7

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJL1-eBFP2

   Plasmid

   #102640

   Purpose

   In vitro expression of eBFP2 from the T7 promoter

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

   Insert

   eBFP2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX SHP-1(NC)-SH2

   Plasmid

   #46496

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHP-1(NC) (PTPN6 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-221

   Promoter

   Tac

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJYS2_crtYf

   Plasmid

   #85544

   Purpose

   Constitutive transcription of crRNA of crtYf, Spectinomycin resistance

   Depositor

   Sheng Yang

   Article

   Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

   Insert

   crRNA of crtYf
   

   Use

   CRISPR; Shuttle vector corynebacterium glutamicum…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   May 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFCiKm7

   Plasmid

   #167521

   Purpose

   Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, KmR, Erythromycin Resistance Marker.

   Depositor

   Christopher Reisch

   Article

   Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

   Insert

   CinRAM-KmR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PCin

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDual-eGFP

   Plasmid

   #63215

   Purpose

   Expression of eGFP in bacteria and in mammalian cells. Used as a reporter for quantifying gene targeting and recombineering in mammalian cells.

   Depositor

   Richard S Myers

   Article

   Valledor et al IUBMB Life. 2012 Aug;64(8):684-9. doi: 1

   Insert

   His-T7-eGFP
   

   Use

   Lentiviral

   Tags

   His6 and T7

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV-EF1α hybrid (CEF)

   Available Since

   April 1, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pCASB

   Plasmid

   #190175

   Purpose

   Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

   Depositor

   Jessica Tyler

   Article

   Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

   Insert

   sgRNA cloning site
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Added 5'-agagacc-3' upstream and 5'…

   Promoter

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pOPINM

    Plasmid

    #26044

    Depositor

    Ray Owens

    Article

    Berrow et al Nucleic Acids Res. 2007 . 35(6):e45.

    Insert

    His-MBP-3C-lacZ
    

    Use

    Tags

    MAHHHHHHSSG_MBP_LEVLFQGP

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available Since

    June 30, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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