33,449 results
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Plasmid#195486PurposeBacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.DepositorInsertHaloTag.v7.AviTag
Tags6xHIS and AviTagExpressionBacterialPromoterT7Available sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
luxA+luxB/pRSETB Duet
Plasmid#197581PurposeExpressing luxA and luxB under dual T7 promoterDepositorInsertLuxA LuxB
TagsT7 tag and 6xHisExpressionBacterialPromoterT7Available sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUS250
Plasmid#198322PurposeCumate-inducible gene expression in diverse gram negative bacteria. Features also include mobilisation via oriT, a gigantic multiple cloning site, and blue/white screening via amilCP chromoproteinDepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromotercumate-inducibleAvailable sinceApril 12, 2023AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
SupD v2
Plasmid#198598PurposePlasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.DepositorInsertSupD
ExpressionBacterialAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc12-HiBiT
Plasmid#198308PurposeProduces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assaysDepositorInsertPVCpnf_pvc12-HiBiT
ExpressionBacterialAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnfΔpvc13
Plasmid#198283PurposeProduces PVCs lacking tail fibersDepositorInsertPVCpnfΔpvc13
ExpressionBacterialAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
Twin-Strep(R)-Tev-GSGS-Ubiquitin
Plasmid#186803PurposeCodon-optimized bacterial expression plasmid. Expresses Twin-Strep(R)-Tev-GSGS-Ubiquitin.DepositorInsertTwin-Strep(R)-Tev-GSGS-Ubiquitin
TagsGS-Strep (WSHPQFER)-GGGSGGGSGGSSA-Strep (WSHPQFER…ExpressionBacterialMutationStrep tag Lysine's mutated to ArginineAvailable sinceMarch 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDule2 - Mj Acd A9
Plasmid#197652PurposeE. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pairDepositorInsertsMj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA
TagsNoneExpressionBacterialMutationY32A, L65D, F108L, Q109E, D158S, L162TPromoterGlnS and lppAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLN-AIDA-Tyr1
Plasmid#192831PurposeIPTG inducible AIDA-Tyrosinase expressionDepositorInsertAIDA-Tyr1
ExpressionBacterialPromoterpL-lacAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM-41-HsPABPC1_J
Plasmid#146644PurposeBacterial Expression of HsPABPc1DepositorInsertHsPABPc1 (PABPC1 Human)
ExpressionBacterialMutationtwo silent mutations compared to the sequence giv…Available sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQmod3C-GG
Plasmid#191350PurposeClostridium expression vector (pCB102 origin, cmR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
ExpressionBacterialPromoterPlacAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-MBP_G4G6 in pRSF
Plasmid#188454PurposeExpress in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.DepositorAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBTK1008 (pSL7)
Plasmid#191003PurposeExpresses red chromoprotein on a broad host range RSF1010 origin plasmidDepositorInsertred chromoprotein
UseSynthetic BiologyExpressionBacterialPromoterCP25Available sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMMLV-mRFP1-P2A-Puro
Plasmid#160228PurposeExpresses mRFP in mammalian cellsDepositorInsertmRFP1-P2A-Puro
UseRetroviralExpressionBacterial and MammalianPromoterLTRAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX-4T1_ATG4B_BamHI_NotI
Plasmid#190862PurposePlasmid for the expression and purification of of ATG4B. Internal reference: SMC1392DepositorAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBA630
Plasmid#186241PurposeRfxCas13d Negative Control (RFP targeting)DepositorInsertpTet-RfxCas13d + crRNA negative control
ExpressionBacterialAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBK.079
Plasmid#187218PurposeExpresses Eco1 RT and Cas1 + Cas2DepositorInsertsRetron Eco1 Reverse Transcriptase
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterJ23115 and T7/lacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pREDawn-StrR-MCS
Plasmid#188979PurposeFor red light-induced bacterial expression of gene of interest (StrR)DepositorTypeEmpty backboneExpressionBacterialAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBFC0625
Plasmid#186691PurposeVcDART under control of Lac promoter, Vc_lacZ_α_1 gRNA, GmR+sfGFP cargoDepositorInsertstnsA
tnsB
tnsC
tnsD
tniQ
cas8-cas5 fusion
cas7
cas6
Vc_lacZ_α_1 Spacer crRNA
Tn7 transposon
GmR+sfGFP VcDART Cargo
ExpressionBacterialPromoterPLacAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJJS002
Plasmid#188325PurposePCR template for repair templates to tag a gene with a GFP and mIAA7 AID degron using homology-directed repair for C. elegans.DepositorInsertLinker::GFP::mIAA7
ExpressionBacterialAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only