30,807 results

1. His6-TEV-mCerulean3-pBluescript

   Plasmid

   #166757

   Purpose

   For purification of mCerulean3 fluorophore

   Depositor

   David Andrews

   Article

   Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

   Insert

   mCerulean3
   

   Tags

   6x Histidine tag, and TEV protease site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR-XL-rAkap9

   Plasmid

   #196867

   Purpose

   Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akap9 (Akap9 Rat)
   

   Expression

   Bacterial

   Promoter

   Lac promoter and T7 promoter

   Available Since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-XIAP

   Plasmid

   #8340

   Depositor

   Jon Ashwell

   Article

   Yang et al Science 2000 May 5;288(5467):874-7.

   Insert

   XIAP (XIAP Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   March 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSCARS1

   Plasmid

   #174868

   Purpose

   Contains TEF1p-TEF1in-hGFP-CYCt cassette and wildtype ARS1

   Depositor

   Zengyi Shao

   Article

   Lopez et al Appl Microbiol Biotechnol. 2021 Aug 6. p

   Insert

   ARS1
   

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. H9 pMalLP

   Plasmid

   #41811

   Depositor

   Chris Garcia

   Article

   Levin et al Nature. 2012 Mar 25;484(7395):529-33. do

   Insert

   H9 Super-2 (IL2 Human)
   

   Tags

   3C protease site, 6x His, and Maltose Binding Pro…

   Expression

   Bacterial

   Promoter

   pTac

   Available Since

   Jan. 8, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pET19b+H3

   Plasmid

   #157807

   Purpose

   Expression of cysteine-free human histone H3 (c111A)

   Depositor

   Michael Rosen

   Article

   Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

   Insert

   H3
   

   Expression

   Bacterial

   Mutation

   C111A

   Promoter

   T7

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHEA

   Plasmid

   #168297

   Purpose

   Bacterial surface display of polypeptides (nanobodies) in E. coli

   Depositor

   Luis Ángel Fernández

   Article

   Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.

   Insert

   Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
   

   Tags

   6xHis, E-tag, and PelB signal peptide

   Expression

   Bacterial

   Promoter

   LacI-Plac

   Available Since

   June 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJUMP21-1A(sfGFP)

   Plasmid

   #126968

   Purpose

   Level 1 vector. sfGFP reporter. Origin R6K (pir conditional).

   Depositor

   Chris French

   Article

   Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   None

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-TEV-TBP6.9F34MF37MF77M

   Plasmid

   #168268

   Purpose

   Expresses human TBP6.9 with the F34M/F37M/F77M triple mutant

   Depositor

   Joseph Wedekind

   Article

   Srivastava et al Crystals (Basel). 2021 Mar;11(3). doi: 1

   Insert

   TBP6.9
   

   Tags

   6xHis tag N-terminus, TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   changed F34M, F37M and F77M

   Promoter

   T7 promotor

   Available Since

   April 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKOR1-mcs

    Plasmid

    #181755

    Purpose

    Allele replacement for Staphylococcus aureus

    Depositor

    Suzan Rooijakkers

    Article

    Stapels et al Proc Natl Acad Sci U S A. 2014 Sep 9;111

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    April 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. SW5

    Plasmid

    #40108

    Depositor

    Michel Maharbiz

    Article

    Huang et al J Biol Eng. 2012 Jul 9;6(1):9.

    Insert

    Bistable switch using dynamic degradation
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBbA5c-MevT-MBI (JBEI-3085)

    Plasmid

    #87950

    Purpose

    the overproduction of the universal building blocks to make isoprenoids (i.e. IPP and DMAPP).

    Depositor

    Taek Soon Lee

    Article

    Alonso-Gutierrez et al Metab Eng. 2013 May 29;19C:33-41. doi: 1

    Insert

    MevT-MBI
    

    Expression

    Bacterial

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGEMTEZ-Kir2.1

    Plasmid

    #32641

    Depositor

    Richard Axel, Joseph Gogos, C. Ron Yu

    Article

    Yu et al Neuron. 2004 May 27;42(4):553-66.

    Insert

    Kir2.1 (Kcnj2 Mouse)
    

    Expression

    Bacterial

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGEM-NLS-Cre-2A-mCherry-FKF

    Plasmid

    #119868

    Purpose

    Donor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

    Depositor

    Tatjana Sauka-Spengler

    Article

    Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

    Insert

    NLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
    

    Tags

    mCherry

    Expression

    Bacterial

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDF_6xHIS_Csx29

    Plasmid

    #197613

    Purpose

    For protein purification of Csx29

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    6-HIS tagged Csx29
    

    Expression

    Bacterial

    Available Since

    Aug. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCP20 PilB

    Plasmid

    #162845

    Purpose

    Expression of PilB with a C-terminal histag

    Depositor

    Katrina Forest

    Insert

    PilB
    

    Tags

    Histidine tag (H6x)

    Expression

    Bacterial

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUS250

    Plasmid

    #198322

    Purpose

    Cumate-inducible gene expression in diverse gram negative bacteria. Features also include mobilisation via oriT, a gigantic multiple cloning site, and blue/white screening via amilCP chromoprotein

    Depositor

    Nicholas Coleman

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    cumate-inducible

    Available Since

    April 12, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pJBL713

    Plasmid

    #140383

    Purpose

    Expresses CtcS protein under the control of T7-lacO promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-lacO-CtcS-T
    

    Tags

    6XHis

    Expression

    Bacterial

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. lacI-PT7-gusA-pBAV1k

    Plasmid

    #30504

    Depositor

    Ichiro Matsumura

    Article

    Murin et al Appl Environ Microbiol. 2011 Oct 21.

    Insert

    beta-glucoronidase (gusA Escherichia coli)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. BASIC_8_RBS34- mCherry -B0015

    Plasmid

    #68142

    Purpose

    storage vector for BASIC part: RBS-RFP(ORF)

    Depositor

    Geoffrey Baldwin

    Article

    Storch et al ACS Synth Biol. 2015 Apr 16.

    Insert

    RBS34-mcherry-B0015
    

    Expression

    Bacterial

    Available Since

    Aug. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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