33,449 results
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Plasmid#173895PurposeTransposon allowing the expression of the fluorescent protein mEos3.2 in multiple mycoplasma species, under the control of the Spiralin promoterDepositorInsertmEos3.2
ExpressionBacterialPromoterSpiralinAvailable sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHYP-NPC-10H
Plasmid#162789PurposeSARS-CoV-2 Nucleocapsid Protein expression in E.coliDepositorInsertNucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
Tags10xHisExpressionBacterialPromoterT7lacAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huErCas12a
Plasmid#174685PurposeExpresses 6xHis-MBP tagged ErCas12aDepositorPublicationInserthuErCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV, NLS and NLS, 6xHisExpressionBacterialMutationQ926R mutation is observed as indicated from the …PromoterT7Available sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-51b-HaloTag11
Plasmid#175519PurposeExpression of HaloTag11 in bacterial cellsDepositorInsertHaloTag11
TagsHisExpressionBacterialAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSIBR003
Plasmid#177666PurposeSIBR Intron 3 (Int3) interrupts the CDS of FnCas12aDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterlacUV5Available sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-Sfp R4-4 mutant
Plasmid#173491PurposeExpression plasmid for mutant R4-4 of Sfp phosphopantetheinyl transferase. R4-4 can use 3′-dephospho CoA for adding the phosphopantetheinyl group to the carrier proteins.DepositorInsertSfp-R44
TagsHis tagExpressionBacterialAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMesh1
Plasmid#175594PurposeLac inducible, AMP Resistant, non labelled gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.DepositorInsertMesh1 (Mesh1 Fly)
ExpressionBacterialMutationCodon optimized for translation in E.coli. AGGAGG…Available sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDonorGFP(Ptr)
Plasmid#175578PurposeDNA donor for transpositionDepositorInsertMini-Tn, GFP cassette
ExpressionBacterialMutationNOAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-MBP-huTsCas12a
Plasmid#174687PurposeExpresses 6xHis-MBP tagged TsCas12aDepositorPublicationInserthuTsCas12a
UseCRISPR and Synthetic BiologyTags6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHAExpressionBacterialPromoterT7Available sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFLxR3
Plasmid#167518PurposeBroad-Host range expression vector, RSF1010 origin, LuxR activator, PLuxB promoter, mRFP, Tetracycline Resistance Marker.DepositorInsertLuxR-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPLuxBAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHS0663 pET45b(+) KraIscB-1 TwinStrep
Plasmid#176536PurposeBacterial expression of KraIscB-1-TwinStrepDepositorInsertKraIscB-1
TagsTwinStrepExpressionBacterialAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSAN-QUE37C
Plasmid#129312PurposeATP measurementDepositorPublicationInsertQUEEN-37C
ExpressionBacterialAvailable sinceOct. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1 ZIKV E
Plasmid#145341PurposeCell-free/mammalian expression vector for Zika virus Envelope protein with N-term eGFPDepositorInsertZIKV Envelope
Tags8xHis and eGFPExpressionBacterialPromoterT7- CMV/CAGAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDT386
Plasmid#174733PurposeExpresses His-tagged DT386, a truncated diphtheria toxin without a receptor-binding domain.DepositorInsertDT386
TagsHistagExpressionBacterialMutationDeleted amino acids 412-560 (the receptor binding…PromoterT5Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDsEndoNA2
Plasmid#174728PurposeExpresses DsRed-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.DepositorInsertDsRed-Monomer
TagsHistagExpressionBacterialPromoterT5Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-TAT-GFP-HiBit
Plasmid#170991PurposeFor bacterial expression of bdSUMO tagged muGFP with TAT peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-TAT-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB223
Plasmid#164127PurposeNew measurement standardDepositorInsertYFP-PP7
ExpressionBacterialPromoterJ23101Available sinceSept. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaM-2
Plasmid#172768PurposeBacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.DepositorInsertLaM-2
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GST-TAX1BP1
Plasmid#171423PurposeFor the purifcation of TAX1BP1 proteinDepositorInsertTAX1BP1 (TAX1BP1 Human)
ExpressionBacterialAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -