32,926 results
-
Plasmid#140700PurposeNBαMs/Rt-IgG1-FcDepositorInsertNBαMs/Rt-IgG1-Fc
ExpressionBacterialAvailable SinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
C1-82 pComb3xss anti-cFos nanobody
Plasmid#145829PurposeBacterial expression of anti-cFos (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-cFos (Human) recombinant llama nanobody C1-82 (FOS L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceJune 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pT7NT*-Bri2 113-231 R221E
Plasmid#138134PurposeExpresseds human Bri2 BRICHOS R221E mutant in E. coliDepositorInserthuman Bri2 BRICHOS R221E (ITM2B Human)
TagsNT* tag derived from spider silk proteinsExpressionBacterialPromoterT7Available SinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFloat.cspA_v1
Plasmid#133071PurposeEntry vector for cloning genes under a cold-shock cspA promoter for expression in bacteria. Version 1.0.DepositorTypeEmpty backboneUseGateway CloningTags3C-His6 and His6-3CExpressionBacterialPromotercspAAvailable SinceMay 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4136461
Plasmid#149538PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
Tags6xHIS;Linker_GGSG;MBP;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4136464
Plasmid#149534PurposeBacterial expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
Tags6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEVExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceMay 14, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFP.R269
Plasmid#138181PurposeExpresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHCKan-yibDp-GFPuv
Plasmid#127078PurposeLow phosphate inducible yibD gene promoter expressing GFPuv reporterDepositorInsertGFPuv
ExpressionBacterialPromoteryibDpAvailable SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046758
Plasmid#145670PurposeBacterial Expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)DepositorInsertSARS-CoV-2 S (surface glycoprotein) (S Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;HAExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGBW-m4046731
Plasmid#145633PurposeBacterial Expression plasmid for SARS-CoV-2 nsp2DepositorInsertSARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
TagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available SinceApril 28, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFP.R268
Plasmid#138180PurposeExpresses the split mCherry reporter interrupted by the gp41-8 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialPromoterP(araBAD) and iPCAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCST-mAvicFP1
Plasmid#129509PurposeExpresses mAvicFP1 constitutively in E. coli (most strains)DepositorInsertmAvicFP1
ExpressionBacterialPromotersynthetic constitutive (stationary phase) promote…Available SinceFeb. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-28a-His6-SUMO-Munc18-1wt
Plasmid#135550PurposeExpress rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA ResinDepositorInsertMunc18-1wt
Tags6xHis tag, thrombin recognition and cleavage site…ExpressionBacterialMutationNo ATG start codonPromoterT7 promoterAvailable SinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
PJQ48
Plasmid#134647PurposeContains Promoter 2 with bsa1 cut sites for inserting sgRNADepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterAnderson BBa_J23114Available SinceJan. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMOD3-proD/Lux-nat
Plasmid#120324PurposeBacterial luminescenceDepositorInsertLux operon
ExpressionBacterialPromoterproDAvailable SinceJan. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHIS-GB1-BP1
Plasmid#133427PurposeHuman BP1 fragment of SREBF1 with GB1 solubility tag in vector for in vitro expression, with T7 promoter.DepositorInsertSREBF1 (SREBF1 Human)
ExpressionBacterialMutationSREBP1a amino acids 17-42 fused to GB1 solubilit…Available SinceJan. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAH-CTX1-rha
Plasmid#129390PurposeDerived from mini-CTX1. An integrase vector that can integrate the rhamnose-inducible promoter system into the chromosome of a host bacterium.DepositorInsertRhamnose-inducible promoter
ExpressionBacterialMutationN/APromoterPrhaBADAvailable SinceDec. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJBL7025
Plasmid#128810PurposeFluoride riboswitch-regulated catechol 2,3-dioxygenaseDepositorInsertFluoride riboswitch-regulated catechol 2,3-dioxygenase
UseSynthetic BiologyExpressionBacterialAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
K560_AviC_coBirA
Plasmid#129765PurposeExpresses Drosophila Kinesin-1 truncated at 560 and biotinylated at tail for single-molecule assaysDepositorInsertKHC (Khc Fly)
TagsAvi-tag GLNDIFEAQKIEWH and His6ExpressionBacterialMutationTruncation at 559Available SinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAD-SPS6-Pman
Plasmid#133906PurposeFluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.DepositorInsertsfGFP(SPS6)
UseE.coli/bacillus spp. shuttle vectorExpressionBacterialPromoterPmanAvailable SinceNov. 1, 2019AvailabilityAcademic Institutions and Nonprofits only