33,449 results
-
Plasmid#87346PurposeCysteine-free (C80S/C574S) S. pyogenes Cas9 expression plasmidDepositorInsertCas9
TagsHis-MBPExpressionBacterialMutationC80S/C574SPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET11a-CA(A14C, E45C)
Plasmid#79045PurposeBacterial vector for cloning and propagation in DH5alphaDepositorInsertCA (A14C, E45C)
ExpressionBacterialMutationA14C and E45C mutations in HIV-1 CA proteinPromoterT7Available sinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYGS
Plasmid#80753PurposeExpression of human gamma S-crystallin in E. coliDepositorAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAP84
Plasmid#86554PurposeExpresses sLucopt luciferase from the constitive Bacillus subtilis promoter Pveg in Clostridium difficileDepositorInsertPveg-sLucopt
UseLuciferase and Synthetic BiologyExpressionBacterialPromoterPvegAvailable sinceFeb. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-CLIP-KanMX6
Plasmid#87025PurposeTag S. pombe genes in C terminal with CLIPDepositorInsertCLIP
ExpressionBacterial and YeastAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-mEGFP-KanMX6
Plasmid#87023PurposeTag S. pombe genes in C terminal with mEGFPDepositorInsertmEGFP
ExpressionBacterial and YeastAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQLinkHD-SYNZIP1
Plasmid#80647PurposeEncodes the synthetic coiled coil peptide SYNZIP1DepositorInsertSYNZIP1
Tags6xHisExpressionBacterialAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pVHed02
Plasmid#82352PurposeBxb1/TP901 temporal logic gate with mKate2 and sfGFP on low-copy plasmidDepositorInsertsmKate2 RFP
Superfolder GFP
Temporal logic gate with TP901 and Bxb1 attachment sites
UseSynthetic BiologyExpressionBacterialAvailable sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
hsDCX-EGFP-pHAT
Plasmid#83918Purposehuman Doublecortin, N-terminal 6-His tag, C-terminal EGFP-Strep-tag IIDepositorInsertDoublecortin isoform b (DCX Human, NM_178152.2)
Tags6-His and EGFP+Strep-tag IIExpressionBacterialAvailable sinceDec. 8, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFJ1320
Plasmid#84829PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and smu-2 intronsDepositorInsertC. elegans codon optimized GFP
ExpressionBacterial and WormPromoterPeft-3Available sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEN25-Ub Hs
Plasmid#83232PurposepHis-par2-Ub HsDepositorAvailable sinceNov. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
AURKA_HUMAN_D0
Plasmid#79739PurposeThis plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.DepositorAvailable sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hygMV
Plasmid#73891Purposefission yeast drug selection markerDepositorInserthygMV
ExpressionBacterial and YeastPromoterpombe TEFAvailable sinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET15b LFn-Fla 3A
Plasmid#84872PurposeBacterial expression of LFn-Fla 3A fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
Fla
TagsHisExpressionBacterialMutation3 C-terminal leucines have been replaced by alani…PromoterT7Available sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMH-macroHC
Plasmid#82337PurposeProvides a C-terminal macro domain and 6xHis tag for expression in E. coliDepositorInsertSPX domain of the Vacuolar Transporter Chaperone 4 (VTC4 Budding Yeast)
Tags6xHis non-cleavable and macro domain of human his…ExpressionBacterialMutationTruncated protein, amino acids 1-178Available sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
SPS-7942
Plasmid#80459PurposeIPTG inducible expressoin of sucrose phosphate synthase derived from Synechococcus elongatus PCC 7942DepositorInsertSucrose phosphate synthase
ExpressionBacterialAvailable sinceOct. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pECJ3B
Plasmid#75466PurposeToggle switch plasmid containing lacI-pdt3BDepositorInsertlacI-pdt3B
ExpressionBacterialPromoterpLtetOAvailable sinceOct. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSucCD2
Plasmid#83324Purposeexpresses E. coli succinyl-CoA synthetaseDepositorInsertsucCD
ExpressionBacterialAvailable sinceSept. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGLO-GFP-3UAG
Plasmid#82501PurposeExpresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153DepositorInsertGreen Fluorescence Protein with 3 UAG codons
ExpressionBacterialMutationAdded an UAG codon at the amino acid position 3, …PromoterAraBADAvailable sinceSept. 22, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHLU
Plasmid#64167Purposeharbours marker genes HIS3, LEU2 and URA3DepositorTypeEmpty backboneExpressionBacterial and YeastAvailable sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by