31,587 results
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Plasmid#209317PurposeExpresses a sgRNA template with a protospacer cloning site flanked by BsaI restriction sites for easy insertion of a targeting cassette.DepositorInsertGroup B Streptococcus-compatible single guide RNA
ExpressionBacterialPromoterXyl/tet promoter that is constitutively activeAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_2A4D
Plasmid#234196PurposeHeterologous protein expression of ubiquitin-conjugating enzyme E2 variant 1 (UEV-1) (CROC-1) in Escherichia coliDepositorAvailable SinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSANG10-3F
Plasmid#39264DepositorTypeEmpty backboneUseBacterial expression, bacterial expression of re…Tags6xHis, pelB leader, and tri-FLAGExpressionBacterialPromoterT7Available SinceOct. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc12-HiBiT
Plasmid#198308PurposeProduces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assaysDepositorInsertPVCpnf_pvc12-HiBiT
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT31
Plasmid#89666Purposeexpression of doubleTAL staple protein dT31 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT31
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB1A3_GFP_dT27
Plasmid#89665Purposeexpression of doubleTAL staple protein dT27 with N-terminally fused cycle3-GFPDepositorInsertGFP_dT27
TagsHis6ExpressionBacterialPromoterT7Available SinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLS.405
Plasmid#187203PurposeExpresses wild-type retron Eco1 ncRNA + Eco1 RT, and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA (wildtype)
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterT7/LacAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-His-Dre
Plasmid#123130PurposeBacterial expression plasmid for wild-type Dre with an N-terminal His-tag.DepositorInsertDre recombinase
Tags6x His tagExpressionBacterialPromoterT7Available SinceApril 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLG020 (AVAST type 4)
Plasmid#157898PurposeExpresses the AVAST type 4 defense systemDepositorInsertAVAST type 4 (STAND ATPase with Mrr-like N-terminal domain)
ExpressionBacterialAvailable SinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLG019 (AVAST type 3)
Plasmid#157897PurposeExpresses the AVAST type 3 defense systemDepositorInsertAVAST type 3 (STAND ATPase with N-terminal DUF4297)
ExpressionBacterialAvailable SinceDec. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMan operon
Plasmid#124888PurposeExpresses proteins encoding Man operon in E. faecalisDepositorInsertmanX1, manX2, manY, manZ, manO, EF0025
ExpressionBacterialAvailable SinceApril 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
JMJD3
Plasmid#39069PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMMLV-EBFP-P2A-Puro
Plasmid#160227PurposeExpresses EBFP in mammalian cellsDepositorInsertEBFP-P2A-Puro
UseRetroviralExpressionBacterial and MammalianPromoterLTRAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pdCas9-omega
Plasmid#114632PurposeAddgene plasmid 44249 where dCas9 has been replaced with dCas9 fused to the omega subunit of RNAPDepositorInsertdCas9-omega
UseCRISPRExpressionBacterialPromoterTet promoterAvailable SinceSept. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_VASP_WH1
Plasmid#109790PurposeProtein expression and purification of VASP_WH1DepositorInsertVASP_WH1 (VASP Human)
ExpressionBacterialAvailable SinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
FHL-cpmTq2-Calcium-lifetime-sensor
Plasmid#129628PurposeGenetically encoded calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsertFHL-cpmTq2-Calcium-lifetime-sensor
Tags6x His - Modified TorA MNNNDLFQASRRRFLAQLG[G to …ExpressionBacterial and MammalianPromoterCMV,&RhamnoseAvailable SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSETB-roGFP1-R12
Plasmid#83311PurposeInducible expression of redox sensitive GFP in bacteriaDepositorInsertroGFP1-R12
Tags6xHISExpressionBacterialMutationC48S/Q80R/S147C/Q204C/N149K/F223R/S202KPromoterT7Available SinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBbE2a-RFP
Plasmid#35322DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterTetAvailable SinceFeb. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
cr3Dd
Plasmid#184862Purposecr3 helper plasmid for recombination and Cas9 induction; harbors the Cas9 endonuclease and a species-specific recombination systemDepositorInsertCas9, bet, exo
TagsCas9 ssrA degradation tagExpressionBacterialAvailable SinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only