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31,059 results

Showing: 6181 - 6200 of 31059 results

  3. SHMT2

    Plasmid
    #25479
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SHMT2 (SHMT2 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    A269T
    Available Since
    Aug. 23, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    25479-plasmid-map-sequence-id-168679

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  5. pPBO.ACT006

    Plasmid
    #182716
    Purpose
    Constituitvely expressed CasRx crRNA cloning vector, truncated 3' terminator region, with eGFP for cloning
    Depositor
    Joseph Schoeniger
    Article
    Otoupal et al Nucleic Acids Res. 2022 Aug 11. pii: 666
    Insert
    CasRx crRNA cloning backbone
    Use
    CRISPR
    Tags
    GFP in cloning site of crRNA for easier screening…
    Expression
    Bacterial
    Mutation
    Catalytically deactivated R295A, H300A, R849A, H8…
    Promoter
    pJ23119 (TTGACAGCTAGCTCAGTCCTAGGTATAATACTAGT)
    Available Since
    Aug. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    182716-plasmid-map-sequence-id-361297

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  9. pMAL-p4X

    Plasmid
    #75289
    Purpose
    Expresses proteins in the periplasm as fusions to a high-binding mutant (A313V) maltose-binding protein
    Depositor
    Paul Riggs
    Article
    Walker et al Appl Microbiol Biotechnol. 2010 Sep;88(1
    Type
    Empty backbone
    Tags
    maltose-binding protein
    Expression
    Bacterial
    Promoter
    tac
    Available Since
    July 19, 2016
    Availability
    Industry, Academic Institutions, and Nonprofits
    75289-plasmid-map-sequence-id-142416

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  11. pMSR

    Plasmid
    #161983
    Purpose
    Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile 630. Uses inducible expression of CD2517.1 toxin as a counter-selection marker
    Depositor
    Olga Soutourina
    Article
    Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    Oct. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    161983-plasmid-map-sequence-id-321920

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  16. pOPINCDE

    Plasmid
    #41135
    Depositor
    Ray Owens
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    March 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41135-plasmid-map-sequence-id-54729

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  17. MSP2292

    Plasmid
    #70707
    Purpose
    Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    T7
    Available Since
    Dec. 23, 2015
    Availability
    Academic Institutions and Nonprofits only
    70707-plasmid-map-sequence-id-127770

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  19. pET21b-MBPx-MBPx-SpyTag003

    Plasmid
    #216291
    Purpose
    Expresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.
    Depositor
    Mark Howarth
    Article
    Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:
    Insert
    MBPx-MBPx-SpyTag003
    Use
    Affinity Reagent/ Antibody
    Tags
    His6 and SpyTag003
    Expression
    Bacterial
    Promoter
    T7 promoter
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    216291-plasmid-map-sequence-id-443903

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  20. pSL0828 (pQCascade_crRNA-4)

    Plasmid
    #130637
    Purpose
    Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from one T7 promoter, and a CRISPR RNA from a second T7 promoter. The CRISPR array encodes a guide RNA that targets lacZ.
    Depositor
    Samuel H. Sternberg
    Article
    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:
    Insert
    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(target-4)
    Use
    CRISPR; Transposon
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    130637-plasmid-map-sequence-id-255021

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Showing: 6181 - 6200 of 31059 results