33,449 results

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pSG86-J23151-LasR

Plasmid

#102456

Purpose

Expresses LasR protein in E. coli. The expression is controlled by a constitutive promoter.

Depositor

Richard Murray

Publication

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

LasR (lasR )

Expression

Bacterial

Promoter

J23151

Available since

Oct. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pMRE152

Plasmid

#118502

Purpose

broad-host range plasmid to constitutively express fluorescent protein genes in bacteria

Depositor

Mitja Remus-Emsermann

Publication

Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

Insert

sGFP2

Expression

Bacterial

Available since

Oct. 30, 2018

Availability

Academic Institutions and Nonprofits only

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pKEW336-MBP-TEV-MbCas12a-33362

Plasmid

#115670

Purpose

Expresses MbCas12 (from M. bovoculi 33362) in bacteria via T7 RNAP, for purification

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

MbCas12a

Use

CRISPR

Tags

MBP-TEV

Expression

Bacterial

Promoter

T7 RNAP

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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His6_BcLOV4_mCherry_BamUK

Plasmid

#114596

Purpose

Encodes mCherry-tagged BcLOV4 for expression in bacterial cells

Depositor

Brian Chow

Publication

Glantz et al Proc Natl Acad Sci U S A. 2018 Aug 14;11

Insert

BcLOV4

Tags

His6 and mCherry

Expression

Bacterial

Promoter

T7

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCF525-EF1a-Hygro-P2A-AcrVA5-lenti

Plasmid

#115664

Purpose

Expresses AcrVA5 in human cells, contained in a lentiviral vector.

Depositor

Jennifer Doudna

Publication

Watters et al Science. 2018 Oct 12;362(6411):236-239.

Insert

AcrVA5

Use

Lentiviral

Expression

Bacterial and Mammalian

Promoter

EF-1a promoter

Available since

Oct. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pOPS0377

Plasmid

#115509

Purpose

Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing sfGFP

Depositor

Philip Poole

Publication

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Constitutive promoter pNeo pOGG001

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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HBT-GCaMP6-HA

Plasmid

#107433

Purpose

For Arabidopsis mesophyll protoplast transient expression assay

Depositor

Jen Sheen

Publication

Liu et al Nature. 2017 May 18;545(7654):311-316. d

Insert

GCaMP6S

Tags

HA

Expression

Bacterial

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

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B1-4

Plasmid

#111466

Purpose

translational elongation factor Tu

Depositor

Cheemeng Tan

Publication

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

translational elongation factor Tu

Tags

6xHis

Expression

Bacterial

Available since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pDJSVT89

Plasmid

#109303

Purpose

Express periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tag

Depositor

Daniel Slade

Publication

Casasanta et al Methods Mol Biol. 2019;1960:123-138. doi

Type

Empty backbone

Tags

TamA 1-27, 6His

Expression

Bacterial

Promoter

T7

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103 NEDD4L WW domain #2

Plasmid

#104205

Purpose

Bacterial expression of WW domain #2 from NEDD4L

Depositor

Sachdev Sidhu, Marius Sudol

Insert

NEDD4L WW domain #2 (NEDD4L Human)

Tags

GST

Expression

Bacterial

Mutation

codon optimized for expression in bacteria

Promoter

tac

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pHAT4

Plasmid

#112585

Purpose

Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag fusions

Depositor

Marko Hyvönen, Johan Peränen

Publication

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Tags

6His

Expression

Bacterial

Available since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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BB3nK_ext_AC

Plasmid

#108686

Purpose

Plasmid for assembly of donor DNA templates consisting of two expression units

Depositor

Brigitte Gasser

Publication

Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pET28b-His-hCE

Plasmid

#112710

Purpose

Expresses N-terminally His-tagged human CE/RNGTT in bacterial cells

Depositor

Daniel Schoenberg

Publication

Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

RNGTT (RNGTT Human)

Tags

6xHis and Stop codons placed after RNGTT sequence…

Expression

Bacterial

Mutation

R594H

Promoter

T7

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-FRT

Plasmid

#113398

Purpose

expresses gRNA for Cas9 FRT targetting

Depositor

Jan Michiels

Publication

Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

Insert

exo, beta, gam, sgRNA-FRT

Use

CRISPR

Expression

Bacterial

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pScaf-10080.1

Plasmid

#111410

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 10080 bases in length

Depositor

Shawn Douglas

Publication

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-ACKN

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pScaf-5544.1

Plasmid

#111405

Purpose

pScaf phagemid with insert for generating DNA origami scaffold 5544 bases in length (v1)

Depositor

Shawn Douglas

Publication

Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

Insert

pScaf-GH'

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.944

Plasmid

#108523

Purpose

PCin-YFP Reporter. OHC14 sensor for use in Marionette strain.

Depositor

Christopher Voigt

Publication

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PCin-YFP

Expression

Bacterial

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pJL1 sortase

Plasmid

#106288

Purpose

Expresses sortase in Ecoli off a T7 promoter

Depositor

James Collins

Publication

Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

Insert

sortase A pentamutant

Expression

Bacterial

Promoter

T7

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGEXNKI-his-GST-3C-LIC

Plasmid

#108709

Purpose

Expression of a GST-6xHis-tagged target protein with 3C protease cleavage site.

Depositor

Titia Sixma

Publication

Luna-Vargas et al J Struct Biol. 2011 Aug;175(2):113-9. do

Type

Empty backbone

Tags

GST-6xHis-3C protease cleavage site

Expression

Bacterial

Promoter

tac

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pTK-enhNC1.1_M3-MCS-2A-eGFP-SV40pA

Plasmid

#110203

Purpose

Recipient vector for Cranial Neural Crest-specific expression of protein of interest

Depositor

Tatjana Sauka-Spengler

Insert

enhNC1.1_M3-TF_MCS-2A-eGFP-SV40pA

Tags

eGFP

Expression

Bacterial

Available since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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