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pLsCas13a-gRNA-NT

Plasmid

#164857

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-NT

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pRAM18dSFA[MCS]

Plasmid

#84685

Purpose

Can be used for the transformation of rickettsia

Depositor

Ulrike Munderloh

Article

Burkhardt et al PLoS One. 2011;6(12):e29511. doi: 10.137

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PtxS1-c003

Plasmid

#173076

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

PtxS1

Expression

Bacterial

Mutation

D35-I221

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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MSP574

Plasmid

#98807

Purpose

Expression Vector

Depositor

David Liu

Article

Packer et al Nat Commun. 2017 Oct 16;8(1):956. doi: 1

Insert

pET28 MBP-ENLYFQS-GST

Expression

Bacterial

Mutation

None

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX SHIP1-SH2

Plasmid

#46492

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SHIP1 (INPP5D Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1-118

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA-32Asp8

Plasmid

#102617

Purpose

Expression of human RPA with aspartic acid substituted phosphorylation domain

Depositor

Marc Wold

Article

Binz et al J Biol Chem. 2003 Sep 12;278(37):35584-9

Inserts

RPA1 (RPA1 Human)
RPA3 (RPA3 )
RPA2 (RPA2 )

Expression

Bacterial

Mutation

with aspartic acid substituted phosphorylation do…

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pFloat.T7_v1

Plasmid

#133070

Purpose

Entry vector for cloning genes under a T7 promoter for expression in bacteria. Version 1.0.

Depositor

Kacper Rogala, David Sabatini

Article

Rogala et al Science. 2019 Oct 25;366(6464):468-475.

Type

Empty backbone

Use

Entry vector

Tags

3C-His6 and His6-3C

Expression

Bacterial

Promoter

T7

Available Since

May 26, 2020

Availability

Academic Institutions and Nonprofits only

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pFS_0414_pET30_MCS-BBaJ23100-LuxR-B0015term-pLux rR-Fluc-rrnBterm-pCat-tetR-Term-ptetA-Rluc-rrnBterm_FsRT-Cas1-Cas2_ARRAY2_FaqI

Plasmid

#117005

Purpose

Expresses FsRT-Cas1-Cas2 under pT7lac, Fluc under pLuxR and Rluc under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

Depositor

Randall Platt

Article

Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

Insert

Fusicatenibacter saccharivorans RT-Cas1-Cas2

Expression

Bacterial

Promoter

T7

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAJM.884

Plasmid

#108536

Purpose

AcuRAM + PAcu-YFP Reporter. Acr sensor for use in non-Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

AcuRAM + PAcu-YFP

Expression

Bacterial

Mutation

AcuR: E80K R154H L202M

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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lacI-PT7-gusA-pBAV1k

Plasmid

#30504

Depositor

Ichiro Matsumura

Article

Murin et al Appl Environ Microbiol. 2011 Oct 21.

Insert

beta-glucoronidase (gusA Escherichia coli)

Tags

His

Expression

Bacterial

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pKESK22

Plasmid

#64857

Purpose

expression vector, low to medium copy number, tac promoter lacI, kanamycin resistance, p15A origin of replication

Depositor

Karin Schnetz

Article

Stratmann et al J Bacteriol. 2008 Feb;190(3):926-35. Epu

Type

Empty backbone

Expression

Bacterial

Promoter

Ptac

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

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pBad-UXPS

Plasmid

#172138

Purpose

bacteria expression plasmid for a UDP/UTP sensor

Depositor

Huiwang Ai

Article

Li et al ACS Cent Sci. 2021 Oct 27;7(10):1763-177

Insert

UXPS

Expression

Bacterial

Promoter

araBAD

Available Since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCHARGE3

Plasmid

#24658

Depositor

Tanya Parish

Article

Carroll et al PLoS One. 2010 . 5(3):e9823.

Insert

Turbo

Expression

Bacterial

Available Since

June 9, 2010

Availability

Academic Institutions and Nonprofits only

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pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu

Plasmid

#216421

Purpose

Empty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.

Depositor

Ellen Bushell

Article

Jonsdottir et al bioRxiv 2024.04.20.590404

Type

Empty backbone

Use

CRISPR; Expression in plasmodium berghei

Tags

3xmyc

Expression

Bacterial

Available Since

April 28, 2024

Availability

Academic Institutions and Nonprofits only

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pPbU6_hdhfr/yfcu

Plasmid

#216422

Purpose

Empty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.

Depositor

Ellen Bushell

Article

Jonsdottir et al bioRxiv 2024.04.20.590404

Type

Empty backbone

Use

CRISPR; Expression in plasmodium berghei

Expression

Bacterial

Available Since

April 28, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SARS-CoV-2 NSP13

Plasmid

#176492

Purpose

Expresses the N-terminal His6-tagged SARS-CoV2 helicase (NSP13) in E coli.

Depositor

Xiaodong Cheng

Article

White et al J Phys Chem Lett. 2020 Nov 5;11(21):9144

Insert

SARS-CoV-2 NSP13 (ORF1ab SARS-CoV)

Tags

His6 and T7

Expression

Bacterial

Mutation

I570 from SARS-CoV NSP13 was mutated to V to conv…

Promoter

T7

Available Since

Jan. 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-PTBP1CCtoSS

Plasmid

#89157

Purpose

Recombinant expression of PTBP1 C250S,C251S in E.coli

Depositor

Frédéric Allain

Article

Dorn et al Nat Methods. 2017 Mar 27. doi: 10.1038/n

Insert

Polypyrimidine Tract Binding Protein 1 mutant Cys250Ser, Cys251Ser (PTBP1 Human)

Tags

hexa His

Expression

Bacterial

Mutation

mutations: Cys 250 to Ser, Cys 251 to Ser

Promoter

T7

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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MP2

Plasmid

#69628

Purpose

Untargetted Bacterial Mutagenesis

Depositor

David Liu

Article

Badran et al Nat Commun. 2015 Oct 7;6:8425. doi: 10.1

Insert

dnaQ926

Expression

Bacterial

Promoter

E. coli PBAD

Available Since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pEB2-mCherry2-L

Plasmid

#104003

Purpose

Plasmid encoding mCherry2-L

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mCherry2-L

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to DsRed (MSKGEE NNLA IIKEF… V…

Promoter

proC

Available Since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pZa pBAD csgA CBDcex pLac Ara csgGEF

Plasmid

#226378

Purpose

Plasmid carries csgA and csgG,E,F genes of curli operon

Depositor

Urartu Seker

Article

Koksaldi et al Biosens Bioelectron. 2024 Aug 10;264:116

Insert

csgA and csgG,E,F genes of curli operon

Expression

Bacterial

Promoter

pBAD

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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