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pRSFDuet-sumo-h-cGASCD(K427E/K428E)

Plasmid

#127162

Purpose

expression of truncated human cGAS protein

Depositor

Thomas Tuschl

Article

Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

Insert

MB21D1 (CGAS Human)

Tags

His-SUMO tag

Expression

Bacterial

Mutation

None

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-A11(2-196, G38R)-Strep

Plasmid

#196209

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-196, G38R)-Strep.

Depositor

Lalit Deshmukh

Article

Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

Insert

Annexin A11 (ANXA11 Human)

Tags

GB1-6xHis-TEV and Strep-II tag

Expression

Bacterial

Mutation

G38R

Available Since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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Human USP30 (64-502, construct 2, MG-26-18)

Plasmid

#110744

Purpose

Bacterial expression of human USP30 (construct 2)

Depositor

David Komander

Article

Gersch et al Nat Struct Mol Biol. 2017 Sep 25. doi: 1

Insert

USP30 (USP30 Human)

Tags

N-His6-3C-GST-3C

Expression

Bacterial

Promoter

T7

Available Since

June 14, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX6P2-p53 (1-90) (72R)

Plasmid

#62081

Purpose

Expression of human p53 (TAD residues 1-90) (72R) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

GST

Expression

Bacterial

Mutation

Contains TAD/PP residues 1-90, 72R

Promoter

tac

Available Since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only
RhoG

Plasmid

#55694

Purpose

expression of mouse RhoG

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoG (Rhog Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only
ClCry4 Y319D

Plasmid

#160649

Purpose

Expression of protein for various experiments including finding interaction partners

Depositor

Joseph Takahashi

Article

Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

Insert

Clcry4 PHR Y319D

Tags

Hexa his

Expression

Bacterial

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pQTEV-UFD1L

Plasmid

#31560

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

ubiquitin fusion degradation 1-like (UFD1 Human)

Tags

His and TEV

Expression

Bacterial

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pETDuet-T7-Ct-YhjH

Plasmid

#79167

Purpose

Dual expression of Ct biosensor and YhjH under individual T7 promoters

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Inserts

yhjH
T7-Spinach2-Ct

Expression

Bacterial

Promoter

T7

Available Since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pET11a_3xFlag-nsp14 (SARS-CoV-2)

Plasmid

#169159

Purpose

To express SARS-CoV-2 nsp14 in E. coli

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-nsp5CS[VATLQ]-nsp14 (ORF1ab SARS-CoV-2)

Tags

3xFlag and nsp5CS[VATLQ]: predicted nsp5 protease…

Expression

Bacterial

Mutation

Codon optimised for E. coli

Promoter

T7

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET28b-DiCas7-11-His

Plasmid

#234482

Purpose

Plasmid for bacterial expression and purification of DiCas7-11 protein (human codon-optimized)

Depositor

Miguel Angel Moreno-Mateos

Article

Moreno-Sanchez et al Nat Commun. 2025 Mar 16;16(1):2591. doi:

Insert

DiCas7-11

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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EcRho

Plasmid

#113121

Purpose

Expresses E. coli Rho

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

Rho

Tags

MGH

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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FKBP1AA-c001

Plasmid

#53671

Purpose

bacterial expression plasmid

Depositor

Nicola Burgess-Brown

Insert

FKBP1AA

Tags

His6-TEV

Expression

Bacterial

Mutation

Bacterial expression for structure determination;…

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
C14orf169

Plasmid

#39114

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

C14orf169 (C14orf169 Human)

Tags

Tev His Flag

Expression

Bacterial

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
human EB1 in PET28a for protein expression

Plasmid

#39297

Depositor

Tim Mitchison, Jennifer Tirnauer

Article

Tirnauer et al Mol Biol Cell. 2002 Oct . 13(10):3614-26

Insert

EB1 (MAPRE1 Human)

Tags

6HIS

Expression

Bacterial

Promoter

T7

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pET_SZ1-RGG-RGG

Plasmid

#124942

Purpose

Expression of SZ1-RGG-RGG

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

SYNZIP1-RGG-RGG (using LAF-1 RGG domain) (laf-1 Nematode)

Expression

Bacterial

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMyCA

Plasmid

#84268

Purpose

A versatile vector for recombinant mycobacterial protein production

Depositor

Young-Hwa Song

Article

Magana Vergara et al Protein Sci. 2017 Aug 31. doi: 10.1002/p

Type

Empty backbone

Tags

His6

Expression

Bacterial

Promoter

Aceteamidase

Available Since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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TRBV5-1

Plasmid

#123414

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV5-1 (TRBV5-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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pCoofy4

Plasmid

#43986

Depositor

Sabine Suppmann

Article

Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

Type

Empty backbone

Tags

His6-MBP

Expression

Bacterial

Promoter

T7

Available Since

Oct. 28, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX6-Smad3 WT

Plasmid

#27010

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Tags

GST

Expression

Bacterial

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pLG018 (AVAST type 2)

Plasmid

#157896

Purpose

Expresses the AVAST type 2 defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

AVAST type 2 (STAND ATPase with uncharacterized helical N-terminal domain)

Expression

Bacterial

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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