31,813 results
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Plasmid#39192PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceApril 21, 2016AvailabilityAcademic Institutions and Nonprofits only
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pBbdCas9S_Psyn-sgRNA500
Plasmid#149640Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloningDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPflaB, PpQE30, PsynAvailable SinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
MBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
Plasmid#135454PurposeBacterial expression of TEVΔ proteaseDepositorInsertMBP-TEVcs(ENLYFQ/G)-His6-TEVΔ(220-242)-R5
TagsMBP, His6ExpressionBacterialMutationS219V mutation improves stability. Truncated form…Promotertac promoterAvailable SinceJan. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBbE0k-RFP
Plasmid#35365DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialAvailable SinceMarch 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pExp-mCherry
Plasmid#112569PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis, Strep II, and mCherryExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
SNX10-PX (1-201)
Plasmid#119091PurposeBacterial expression of human phox homology (PX) domain, SNX10-PX (1-201)DepositorAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
GC1 pComb3xss anti-Gephyrin nanobody
Plasmid#134720PurposeBacterial expression of anti-Gephyrin (Human) recombinant llama nanobody for use as an immunolabelDepositorInsertanti-Gephyrin (Human) recombinant llama nanobody GC1 (GPHN L. glama (llama))
Tags6xHis-HAExpressionBacterialPromoterlacAvailable SinceDec. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBRG2
Plasmid#149474PurposeBroad-Host range expression vector, pBBR origin, RhaR-RhaS activator, PrhaBAD promoter, GFPmut3b, Kanamycin Resistance Marker.DepositorInsertRhaS-RhaR-GFP
UseSynthetic BiologyExpressionBacterialPromoterPrhaBADAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-LOC57019
Plasmid#34811DepositorAvailable SinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
DT037A
Plasmid#159398PurposeConstitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23101 (strong) promoterDepositorInsertpr.J23101-sfGFP
UseSynthetic BiologyTagsFLAGExpressionBacterialPromoterJ23101 (strong) promoterAvailable SinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
SB_loop
Plasmid#110103PurposepBXPHM3 with scaffold Sybody of the loop libraryDepositorInsertscaffold Sybody of the loop library
TagsHisExpressionBacterialPromoterPBADAvailable SinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-SpyCatcher003-TEVs-SpyTag003DA
Plasmid#216283PurposeExpresses SpyCatcher003-TEV site-SpyTag003DA in bacterial cytoplasm, to allow TEV protease to remove SpyTag003DA and thereby uncage SpyCatcher003 reactivity.DepositorInsertSpyCatcher003-TEVs-SpyTag003DA
TagsHis6 and TEV cleavage siteExpressionBacterialPromoterT7 promoterAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p1-GST-ArcΔCTD
Plasmid#119878PurposeBacterial Expression construct for the production and purification of Arc missing C-terminal DomainDepositorInsertActivity Regulated Cytoskeletal Protein (Arc Rat)
TagsGSTExpressionBacterialMutationDeletion of nt 832-1110PromotertacAvailable SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMH610
Plasmid#131862PurposeProduces holo-PhyB* in E. coliDepositorInsertsPhyB(1-651)-RGD-His6-Cys
HO1
PcyA
ExpressionBacterialPromoterT7 promoter-1 and T7 promoter-2Available SinceOct. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKL11-GCaMP6f bb100
Plasmid#158983PurposeConstitutive expression (sigma 70 promoter) of GCaMP6f fluorophore in E. coli.DepositorInsertGCaMP6f
ExpressionBacterialMutationcodon optimized for e. coli from Addgene#100833PromoterBba_J23100 (sigma 70)Available SinceSept. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBS PKC theta
Plasmid#8426DepositorInsertMouse Protein Kinase C theta (Prkcq Mouse)
ExpressionBacterialAvailable SinceNov. 22, 2005AvailabilityAcademic Institutions and Nonprofits only -
CLK1
Plasmid#39149PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMAL-c5e-Rubicon RH
Plasmid#216706PurposeBacterial protein expression of Rubicon RH domainDepositorAvailable SinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-UbE2F
Plasmid#15800DepositorAvailable SinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pACRISPR-CM
Plasmid#203588PurposeA sgRNA expression plasmid for genome editing in BurkholderiaDepositorTypeEmpty backboneExpressionBacterialAvailable SinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only