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32,417 results

Showing: 6621 - 6640 of 32417 results

  1. pGEX4T-1-6AlanineHE1

    Plasmid
    #165098
    Purpose
    bacterial expression of a GST fusion of Human Uba1 with a 6alanine linker between the GST encoded by the plasmid and the inserted Uba1
    Depositor
    Arthur Haas
    Insert
    Uba1 (UBA1 Human)
    Tags
    GST-6-alanine
    Expression
    Bacterial
    Mutation
    None
    Available Since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165098-plasmid-map-sequence-id-326697

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  3. pRSET.HaloTag.v7.CoOp.Cys-less

    Plasmid
    #195487
    Purpose
    Bacterial expression plasmid with HaloTag v7 insert; modified to replace cysteines C61, C262, and optimized to human codon bias/preference for use in oxidizing environments.
    Depositor
    Tim Brown, HHMI-JRC Tool Translation Team
    Insert
    HaloTag.v7
    Tags
    6xHIS
    Expression
    Bacterial
    Mutation
    C61T, C262V
    Promoter
    T7
    Available Since
    May 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    195487-plasmid-map-sequence-id-393190

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  4. pBad-CA TAG126

    Plasmid
    #105836
    Purpose
    Expresses thermostable variant of human carbonic anhydrase II with TAG stop codon at site 126 and C-terminal 6x his tag
    Depositor
    Ryan Mehl
    Article
    Mehl et al 2020
    Insert
    human carbonic anhydrase II thermostable variant (CA2 Human)
    Tags
    6x his tag
    Expression
    Bacterial
    Mutation
    TAG stop codon at site 126
    Promoter
    pBad/arabinose
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    105836-plasmid-map-sequence-id-205516

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  5. JMJD1BA-c068

    Plasmid
    #53627
    Purpose
    bacterial expression plasmid
    Depositor
    Nicola Burgess-Brown
    Insert
    JMJD1BA
    Tags
    TEV-His6-Flag
    Expression
    Bacterial
    Mutation
    Bacterial expression for structure determination;…
    Promoter
    T7
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  7. pTNS2

    Plasmid
    #87802
    Purpose
    For constructing NusA-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.
    Depositor
    Yu-Zhu Zhang
    Article
    Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167
    Type
    Empty backbone
    Tags
    6xHis and Strep II
    Expression
    Bacterial
    Available Since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87802-plasmid-map-sequence-id-177263

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  8. pET28a(+)/FbGH17A

    Plasmid
    #100911
    Purpose
    Expression of the Beta-1,3-Endo-Laminarinase FbGH17A in E.coli
    Depositor
    Jan-Hendrik Hehemann
    Insert
    FbGH17A
    Tags
    His-Tag
    Expression
    Bacterial
    Available Since
    March 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    100911-plasmid-map-sequence-id-193697

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  13. pEND-164_Cacnes_pFAST

    Plasmid
    #225616
    Purpose
    Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.
    Depositor
    Marc Güell
    Article
    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi
    Insert
    pFAST
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Available Since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225616-plasmid-map-sequence-id-451365

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  16. pDule2-Tet2.0

    Plasmid
    #85497
    Purpose
    Plasmid for incorporating the non-canonical amino acid Tetrazine 2.0 with the Mj Tet2.0 synthetase and cognate amber suppressing tRNA in Ecoli.
    Depositor
    Ryan Mehl
    Article
    Blizzard et al J Am Chem Soc. 2015 Aug 19;137(32):10044
    Insert
    Tetrazine2.0 tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    Expression
    Bacterial
    Mutation
    Y32G L65Q F108S Q109D D158S L162N
    Promoter
    lpp (constitutive)
    Available Since
    Jan. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    85497-plasmid-map-sequence-id-169854

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Showing: 6621 - 6640 of 32417 results