30,808 results

1. pTrcHisA-PPARalpha(aa101-468)

   Plasmid

   #13574

   Depositor

   Martin Scanlon

   Insert

   human peroxisome proliferator activated receptor alpha A/B domain deletion construct (aa101-468) (PPARA Human)
   

   Tags

   x6His N-terminal, enterokinase cleavage site

   Expression

   Bacterial

   Mutation

   deleted residues 1-100.

   Available Since

   March 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOPINNUSA

   Plasmid

   #41119

   Depositor

   Ray Owens

   Article

   Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

   Type

   Empty backbone

   Expression

   Bacterial, Insect, and Mamm…

   Available Since

   March 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 1457_pDEST_miniTol2_R4-R2_Cryst_mCherry

   Plasmid

   #171796

   Purpose

   pDEST miniTol2-clone for R4-R2 3-component gateway assembly (p5E + pME). Include a mCherry selection marker (Crystallin promoter Lens/Eyes)

   Depositor

   Jean Giacomotto

   Article

   Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Jan. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMRE-Tn5-143

   Plasmid

   #118527

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sYFP2
   

   Use

   Minitn5 delivery vector

   Expression

   Bacterial

   Available Since

   July 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRU1701

   Plasmid

   #14461

   Depositor

   Philip Poole

   Article

   Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

   Insert

   gfp+
   

   Expression

   Bacterial

   Available Since

   April 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX-4T1-14-3-3 gamma GST

   Plasmid

   #13280

   Depositor

   Michael Yaffe

   Article

   Rittinger et al Mol Cell. 1999 Aug . 4(2):153-66.

   Insert

   14-3-3 gamma (Ywhag Rat)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Oct. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pMUT2-VtrA-mCherry

   Plasmid

   #192862

   Purpose

   pMUT2 based vector for sensing human secondary bile acids in E. coli Nissle 1917

   Depositor

   Morten Sommer

   Article

   Vaaben et al ACS Synth Biol. 2022 Dec 16;11(12):4184-

   Insert

   VtrA-CadC fusion protein and cognate promoter inducible by secondary bile acids
   

   Expression

   Bacterial

   Available Since

   April 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAbaMCi-GFP-BsaI_pJMP2748

   Plasmid

   #208888

   Purpose

   Acinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).

   Depositor

   Jason Peters

   Article

   Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

   Insert

   dCas9, sgRNA expression cassette, sfGFP
   

   Use

   Pir dependent origin of replication

   Expression

   Bacterial

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. WT A1-LCD +NLS

   Plasmid

   #178658

   Purpose

   Bacterial expression of N-terminally 6His tagged WT A1-LCD (LCD: low-complexity domain, aa 186-320)

   Depositor

   Tanja Mittag

   Article

   Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

   Insert

   WT A1-LCD +NLS (HNRNPA1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Available Since

   March 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKB231

    Plasmid

    #170018

    Purpose

    Encodes LacI-controlled expression of surface-displayed cecropin P1 and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    Cecropin P1
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Promoter

    Ptac and PvirK

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. SIRT6

    Plasmid

    #28271

    Depositor

    Cheryl Arrowsmith

    Insert

    SIRT6 (SIRT6 Human)
    

    Tags

    His tag with thrombin cleavage

    Expression

    Bacterial

    Available Since

    Feb. 10, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

12. pBBR5pemIKpKan

    Plasmid

    #129271

    Purpose

    Broad host-range plasmid with toxin-antitoxin system for maintenance in the absence of antibiotic selection, and constitutive promoter

    Depositor

    Lindsey Burbank

    Article

    Burbank et al Microbiol Resour Announc. 2019 Sep 5;8(3

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. NFAT5 luciferase

    Plasmid

    #14110

    Depositor

    Anjana Rao

    Article

    Jauliac et al Nat Cell Biol. 2002 Jul . 4(7):540-4.

    Insert

    NM_001113178.2 (NFAT5 Human)
    

    Use

    Luciferase

    Expression

    Bacterial

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

14. CD Ugi3

    Plasmid

    #177195

    Purpose

    Greengate entry vector pGGC000 modified to contain bladderwort insulator 3

    Depositor

    Wayne Parrott

    Article

    Park et al bioRxiv 2021.11.04.467280

    Insert

    Utricularia gibba insulator 3
    

    Expression

    Bacterial

    Available Since

    June 28, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. pRetroX-PTuner DD-linker-M.EcoGII-v5-Lamin B1

    Plasmid

    #122083

    Purpose

    Express M.EcoGII fused to LaminB1 with detabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII-V5-LaminB1
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of LaminB1

    Expression

    Bacterial and Mammalian

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. D. vulgaris sp2 CRISPR/pACYCDuet-1

    Plasmid

    #81186

    Purpose

    Expresses CRISPR array containing 3 copies of Desulfovibrio vulgaris spacer 2 and flanking repeats in E. coli

    Depositor

    Jennifer Doudna

    Article

    Hochstrasser et al Mol Cell. 2016 Sep 1;63(5):840-51. doi:

    Insert

    Synthetic CRISPR array (3 copies of D. vulgaris spacer #2 flanked by repeats)
    

    Use

    CRISPR

    Tags

    None

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET-jAspSnFR3

    Plasmid

    #203458

    Purpose

    jAspSnFR3 in T7 bacterial expression vector

    Depositor

    Lucas Sullivan

    Article

    Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

    Insert

    jAspSnFR3
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28b-NM23-H1

    Plasmid

    #157987

    Purpose

    Expresses NM23-H1 in E.coli cell

    Depositor

    Byung Il Lee

    Article

    Han et al Bulletin of the Kor Chem Soc 2010; 31(5)

    Insert

    NM23-H1 (NME1 Human)
    

    Expression

    Bacterial

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDule-3-nitroTyrosine (A7)

    Plasmid

    #174078

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Expression

    Bacterial

    Mutation

    Y32H H70T D158H I159A L162R

    Promoter

    GlnRS (constitutive)

    Available Since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGlu57

    Plasmid

    #211560

    Purpose

    Amber Suppressor tRNA for Glutamate

    Depositor

    Aron Fenton, Liskin Swint-Kruse

    Insert

    tRNAGlu
    

    Expression

    Bacterial

    Promoter

    Synthetic (Miller 1986)

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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