31,591 results
-
-
pEE075
Plasmid#176832PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert714
Tags6xHisExpressionBacterialPromoterT7Available SinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-NADPH-LUCID-3 (C50 1.4 uM)
Plasmid#117910PurposePlasmid encoding the bacterial expression of the BRET semi-synthetic biosensor measuring the NADPH concentration with a C50 of 1.4 uMDepositorInsertSNAP_Pro30_[cpNLuc23_24]eDHFR(R44A)
TagsHis-tag and Strep-tagExpressionBacterialMutationR44A on eDHFRPromoterT7Available SinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET3aTr
Plasmid#64008PurposeSingle donor or transfer plasmid for source of translation cassette for plasmid suiteDepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
RNF146-c005
Plasmid#173091PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pROP-IP-FBFP
Plasmid#122138PurposeFMN-based fluorescent protein Reporter plasmid for B-Rex contain imperfect operator sequence (anaerobic purpose)DepositorInsertFBFP
ExpressionBacterialPromoterJ23101Available SinceMarch 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
"AND-gate" receiver plasmid
Plasmid#127676PurposeEncodes the transcriptional activator LuxR and GFP. LuxR has an pLlacO-1 promoter and the GFP has a pLux promoter.DepositorInsertLuxR, GFP
UseSynthetic BiologyExpressionBacterialPromoterpLlacO-1 for LuxR, pLux for GFPAvailable SinceJuly 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CATdeltaHD
Plasmid#174811PurposeBacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomainDepositorInsertPARP1-CATdeltaHD (PARP1 Human)
Tags6xHisExpressionBacterialMutationResidues 678-787 replaced by eight-residue linker…Available SinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMM441
Plasmid#61271PurposeAlso referred to as mRGNndm-1. Expresses Cas9, tracrRNA and a guide RNA which target the NDM-1 gene. Contains an R1162 origin and chloramphenicol resistance. Can be mobilized by S17-1 lambda pir.DepositorInsertCas9, tracrRNA, crRNA
UseCRISPRTags6xHisExpressionBacterialAvailable SinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNO183
Plasmid#101068PurposeppsR2-bphP1 bicistronic operon plasmidDepositorInsertsUseSynthetic BiologyExpressionBacterialMutationinsert guanine at position 430 to fix frameshift …Available SinceNov. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAT205
Plasmid#134882PurposeBacterial expressionDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKEK3395
Plasmid#234079PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertYukonOFP
ExpressionBacterialPromoterpJ23100-BsaIAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-22b(+) pCopA RBS30 mscarlet
Plasmid#226370PurposePlasmid carries copper sensor circuit genes for whole-cell sensing of copper ions with RBS30DepositorInsertmScarlet
Tags6x HistagExpressionBacterialPromotercopA promoter (pCopA)Available SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
Plasmid#198450PurposeProduces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody; also contains truncated spike tip protein (Pvc10).DepositorInsertPVCpnf_pvc13-antiMouseMHCIINb_Δpvc10
ExpressionBacterialAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 stat
Plasmid#112886PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertstat
Tags6xHisExpressionBacterialPromoterPblaAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLZ12Km2-P23R:TA:ffluc
Plasmid#88900PurposeToxin-antitoxin stabilized bioluminescent reporter plasmid for Streptococcus expressionDepositorInserttoxin-antitoxin + firefly luciferase
UseLuciferaseExpressionBacterialAvailable SinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
TS_pLuxLac
Plasmid#140426PurposeToggle switch circuit responsive to AHL gradient.DepositorInsertsGFP
LacI
TetR
mCherry
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTPH413
Plasmid#185728PurposeBacterial PAM profiling of adenine base editor variantsDepositorInsertsecTadA(8e)-neNme2-C
Nme2Cas9 sgRNA
UseCRISPRExpressionBacterialMutationNme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…Available SinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAT207
Plasmid#134884PurposeBacterial expressionDepositorInsertmRFP1
UseSynthetic BiologyExpressionBacterialAvailable SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAWP78-PVCpnf_pvc13-ALFA
Plasmid#198301PurposeProduces PVCs retargeted with ALFA tagDepositorInsertPVCpnf_pvc13-ALFA
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only
