30,327 results

1. pMyrPalm_mEGFP_IRES_puro2b

   Plasmid

   #21038

   Depositor

   Daniel Gerlich

   Article

   Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

   Insert

   pMyrPalm
   

   Use

   Tags

   mEGFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 13, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pPbcsx28

   Plasmid

   #89909

   Purpose

   Expresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoter

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   csx28
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. NLS-tdTomato

   Plasmid

   #163443

   Purpose

   Bacterial expression of His-tagged, SV40 NLS-tagged tdTomato in the modified pRSETB protein expression backbone

   Depositor

   Sanford Simon

   Insert

   tdTomato
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pQE80L-SpyCatcher-ELP-GFP

   Plasmid

   #69835

   Purpose

   Bacterial expression plasmid containing SpyCatcher-ELP-GFP fusion. SpyCatcher forms a covalent bond with SpyTag and can be used to label plasma membrane localized SpyTag-C1C2 in live cells.

   Depositor

   Viviana Gradinaru

   Article

   Bedbrook et al Chem Biol. 2015 Aug 20;22(8):1108-21. do

   Insert

   SpyCatcher-ELP-GFP
   

   Use

   Tags

   6x His Tag, GFP, and TEV Tag

   Expression

   Bacterial

   Mutation

   Promoter

   T5 promoter/lac operator element

   Available since

   July 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTHSSe_52

   Plasmid

   #109250

   Purpose

   ColE1 BbsI cloning vector

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PJ23105

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. LHP2691 - pET30-Ub-K63A

   Plasmid

   #32179

   Depositor

   Linda Hicke

   Insert

   synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   K63A

   Promoter

   T7

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

7. LHP2690 - pET30-Ub-K48A

   Plasmid

   #32178

   Depositor

   Linda Hicke

   Insert

   synthetic yeast Ubiquitin (UBA1, UBA2 Budding Yeast)
   

   Use

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   K48A

   Promoter

   T7

   Available since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

8. P-BT3332

   Plasmid

   #188110

   Purpose

   pBolux harboring the promoter region upstream of BT3332

   Depositor

   Guy Townsend

   Article

   Modesto et al Nat Commun. 2023 Jan 17;14(1):275. doi:

   Insert

   The B. thetaiotaomicron BT3332 promoter cloned into pBolux
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BT3332

   Available since

   Jan. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET_SZ1-RGG-GFP-RGG

   Plasmid

   #124937

   Purpose

   Expression of SZ1-RGG-GFP-RGG

   Depositor

   Daniel Hammer, Matthew Good, Benjamin Schuster

   Article

   Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

   Insert

   SYNZIP1-RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-NEMO

    Plasmid

    #11965

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    NEMO (IKBKG Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. MacroH2A1.1-c002

    Plasmid

    #173087

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    MACROH2A1 (MACROH2A1 Human)
    

    Use

    Tags

    mCerulean

    Expression

    Bacterial

    Mutation

    Q162-N369, codon optimized

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a His tag-FRB-Venus C

    Plasmid

    #100980

    Purpose

    Encodes half of a protein delivery sensor (His-FRB-Venus C) for expression in and purification from Bacteria

    Depositor

    Heinrich Leonhardt

    Article

    Chiu et al Nucleus. 2018 Sep 14. doi: 10.1080/19491

    Insert

    His-FRB-Venus C
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pOPINHALO7

    Plasmid

    #41117

    Depositor

    Ray Owens

    Article

    Bird et al Methods. 2011 Sep;55(1):29-37. Epub 2011

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    Available since

    March 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pV1F

    Plasmid

    #205084

    Purpose

    Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.

    Depositor

    Suomeng Dong

    Article

    Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.

    Insert

    AtVIP1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    agorbacterium virB operon promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-2T SHP1 WT

    Plasmid

    #8594

    Depositor

    Ben Neel

    Article

    Plutzky et al Proc Natl Acad Sci U S A 1992 Feb 1;89(3

    Insert

    SHP1 (PTPN6 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 21, 2005

    Availability

    Academic Institutions and Nonprofits only

16. pUC18T-mini-Tn7T-Zeo-eyfp

    Plasmid

    #65039

    Purpose

    ZeoR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    eyfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPro33

    Plasmid

    #17810

    Depositor

    Jay Keasling

    Article

    Lee et al Appl Environ Microbiol. 2005 Nov . 71(11

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 28, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCOLADuet-1_nsp10 (SARS-CoV-2)

    Plasmid

    #169158

    Purpose

    To express SARS-CoV-2 nsp10 in E. coli

    Depositor

    John Diffley

    Article

    Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

    Insert

    nsp10 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    Methionine

    Expression

    Bacterial

    Mutation

    Codon optimised for E. coli

    Promoter

    T7

    Available since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHH0103_RBM17_RRM

    Plasmid

    #109786

    Purpose

    Protein expression and purification of RBM17_RRM

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    RBM17_RRM (RBM17 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWJ68

    Plasmid

    #46571

    Purpose

    Same as pdCas9, but with the w-dCas9 fusion (w is fused at the N-terminal end of dCas9)

    Depositor

    Luciano Marraffini

    Article

    Bikard et al Nucleic Acids Res. 2013 Jun 12.

    Inserts

    tracrRNA
    w-dcas9
    CRISPR array
    

    Use

    CRISPR

    Tags

    rpoZ

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    Available since

    Aug. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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