30,808 results

1. pET LIC cloning vector with BioBrick polycistronic restriction sites (9A)

   Plasmid

   #48283

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETDUET-GST AO7REwt

   Plasmid

   #213935

   Purpose

   GST tagged AO7 encoding aa 126-258 for expression in bacteria

   Depositor

   Allan Weissman

   Article

   Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

   Insert

   AO7 aa 126-258 (RNF25 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   AO7 cloned into the first multiple cloning site o…

   Promoter

   T7 promoter-1

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pThermoCas9i_ctrl

   Plasmid

   #100982

   Purpose

   Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module

   Depositor

   John van der Oost

   Article

   Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:

   Inserts

   Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
   ThermoCas9 single guide RNA expressing module
   

   Tags

   None

   Expression

   Bacterial

   Mutation

   changed aspartate 8 to alanine and histidine 582 …

   Promoter

   B. coagulans DSM 1 pta promoter and B. smithii xy…

   Available Since

   Nov. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-51b(+)-Caprola_05

   Plasmid

   #194666

   Purpose

   Protein production of the split HaloTag based calcium recorder Caprola_05 in bacteria

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_05
   

   Tags

   His-tag and Strep-tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pACRISPR-TP

   Plasmid

   #203589

   Purpose

   A sgRNA expression plasmid for genome editing in Burkholderia

   Depositor

   Leonilde Moreira

   Article

   Ferreira et al Appl Environ Microbiol. 2024 Feb 21;90(2

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbE7k-RFP

   Plasmid

   #35315

   Depositor

   Jay Keasling

   Article

   Lee et al J Biol Eng. 2011 Sep 20;5:12.

   Insert

   RFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMW96

   Plasmid

   #178066

   Purpose

   Expression of His6-SUMO-TEV-GFP-PreScission-γ-TuNA in E. coli

   Depositor

   Tarun Kapoor

   Article

   Wieczorek et al Cell. 2020 Jan 9;180(1):165-175.e16. doi

   Insert

   CDK5RAP2 (CDK5RAP2 Human)
   

   Expression

   Bacterial

   Available Since

   Dec. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGEX6P1-human full-length LIC1

   Plasmid

   #74597

   Purpose

   used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag

   Depositor

   Ron Vale

   Article

   Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

   Insert

   full length human dynein light intermediate chain 1 (DYNC1LI1 Human)
   

   Tags

   N-terminal GST and Strep II

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEThT-SAHS1

   Plasmid

   #90025

   Purpose

   To express His-tagged SAHS1 in bacteria

   Depositor

   Takekazu Kunieda

   Article

   Yamaguchi et al PLoS One. 2012;7(8):e44209.

   Insert

   SAHS1
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD_mCherry

    Plasmid

    #187387

    Purpose

    arabinose-inducible expression of mCherry

    Depositor

    Roi Avraham

    Insert

    mCherry
    

    Expression

    Bacterial

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTW170b2

    Plasmid

    #136929

    Purpose

    Directed evolution of SpCas9

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    gVI
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-Bst-LF-Idaho

    Plasmid

    #170469

    Purpose

    Expresses His-tagged Bst-LF-Idaho in E. coli

    Depositor

    Jonathan Schmid-Burgk

    Insert

    Bst-LF-Idaho
    

    Tags

    N-terminal His-tag

    Expression

    Bacterial

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET20b-mPTER

    Plasmid

    #222189

    Purpose

    Bacterial expression of mouse PTER protein

    Depositor

    Jonathan Long

    Article

    Wei et al bioRxiv [Preprint]. 2024 Mar 22:2024.03.

    Insert

    PTER (Pter Mouse)
    

    Tags

    6xHis and Strep

    Expression

    Bacterial

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJH776_pJH_LldR_PlldR-sfGFP.3opal

    Plasmid

    #163735

    Purpose

    Lactate-inducible expression of green fluorescent protein encoding three opal codons

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    lldr gfp.3TGA
    

    Expression

    Bacterial

    Mutation

    gfp with first three leucine codons mutated to am…

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJH726_pJH_LldR_PlldR-sfGFP.15TAG

    Plasmid

    #163730

    Purpose

    Lactate-inducible expression of green fluorescent protein encoding fifteen amber codons

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    lldr gfp.15TAG
    

    Expression

    Bacterial

    Mutation

    gfp with first 15 leucine codons mutated to amber

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJH773_pTech_PlldR-HHRz-supP(opal)

    Plasmid

    #163732

    Purpose

    Lactate-inducible expression of supP(opal), preceded by a hammerhead ribozyme

    Depositor

    Matthew Bennett

    Article

    Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

    Insert

    hhrz-supP
    

    Expression

    Bacterial

    Available Since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJPC12-ΔPS-mCherry-PCS/PM,CS-GFP

    Plasmid

    #134357

    Purpose

    Reporter plasmid

    Depositor

    Mark Isalan

    Article

    Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

    Inserts

    GFP
    mCherry
    

    Expression

    Bacterial

    Available Since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. His-Tev-WWP2

    Plasmid

    #186807

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.

    Depositor

    Lalit Deshmukh

    Article

    Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

    Insert

    His-Tev-WWP2 (WWP2 Human)
    

    Tags

    His6-Tev

    Expression

    Bacterial

    Available Since

    March 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pETM-11-6His-DmBelle_B

    Plasmid

    #145950

    Purpose

    Bacterial Expression of DmBelle

    Depositor

    Elisa Izaurralde

    Insert

    DmBelle (bel Fly)
    

    Expression

    Bacterial

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEMF037

    Plasmid

    #157634

    Purpose

    Batis maritima MHT

    Depositor

    Jonathan Silberg

    Article

    Fulk et al ACS Synth Biol. 2020 Nov 20;9(11):3104-3

    Insert

    MHT
    

    Expression

    Bacterial

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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