32,069 results
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Plasmid#126994PurposeLevel 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only
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pJUMP29-1D'(sfGFP)
Plasmid#126981PurposeLevel 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialMutationNoneAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1-INTS3-FL
Plasmid#128415PurposeExpress INTS3 full-length in E. coli with a GST tag (N-terminal)DepositorAvailable SinceAug. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJKR-H-acuR
Plasmid#62567PurposeAcrylate biosensor. High copy plasmid that expresses GFP proportional to acrylate concentration.DepositorInsertGFP
ExpressionBacterialAvailable SinceAug. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRelA'
Plasmid#175595PurposeTet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.DepositorInsertCatalytic domain of E.coli relA gene.
ExpressionBacterialMutationOnly the catalytic domain of the enzyme is used (…Available SinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET E. coli expression vector with BioBrick polypromoter restriction sites (14-A)
Plasmid#48307DepositorTypeEmpty backboneExpressionBacterialAvailable SinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFVK5
Plasmid#167522PurposeBroad-Host range expression vector, RSF1010 origin, VanRAM repressor, PVanCC promoter, mKelly2, Gentamicin Resistance Marker.DepositorInsertVanRAM-mKelly2
UseSynthetic BiologyExpressionBacterialPromoterPVanCCAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBR1_DVK_AE(J23100-RiboJ-UTR1-E0040m-B0015)
Plasmid#197662PurposeExpresses GFP under the control of J23100 and RiboJ-UTR1DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialAvailable SinceJune 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
APLF-c001
Plasmid#173077PurposeExpression of recombinant fusion protein in E. coliDepositorAvailable SinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
bdSUMO-E5TAT-GFP-HiBit
Plasmid#170993PurposeFor bacterial expression of bdSUMO tagged muGFP with E5TAT peptide and NanoLuc complementation peptide variant #86DepositorInsert14x-His-bdSUMO-E5TAT-muGFP-HiBiT
UseLuciferaseExpressionBacterialPromoterT7Available SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET_GFP-SZ2
Plasmid#124931PurposeExpression of GFP-SZ2DepositorInsertGFP-SYNZIP2
TagsGFP and HisExpressionBacterialMutationSZ2 domainAvailable SinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pspCas9-Klenow-ST2-com-vector
Plasmid#176235PurposeVector plasmid for expressing spCas9-Klenow fusion protein and sgRNA. There are two copies of HBB 3’ UTR in the 3’UTR of spCas9-Klenow to enhance expression.DepositorTypeEmpty backboneExpressionBacterialAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYFP
Plasmid#175592PurposeTet inducible, KAN Resistant, mVenus gene on a low copy backbone. Used as a negative control to the pRelA_YFP plasmid.DepositorInsertmVenus
ExpressionBacterialMutationAn improved monomeric YFP. See doi: 10.1021/bi051…Available SinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
PA-DAD-wt
Plasmid#53095PurposeExpresses wild type PA-DAD in mammalian cells, an optogenetic tool to activate endogenous diaphanous related formins in live cells using light.DepositorInsertLOV(wt)-6aa-DAD
Tags6x His and mVenusExpressionBacterial, Insect, and Mamm…MutationL47F and C49S in VenusAvailable SinceAug. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
HECTD1
Plasmid#32860PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertHECTD1 (HECTD1 Human)
TagsHis (with TEV cleavage)ExpressionBacterialMutationamino acids 1879-1966 onlyAvailable SinceJan. 6, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSFDuet1-Cdc45
Plasmid#126878PurposeExpresses human Cdc45 in bacteriaDepositorAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCD185
Plasmid#113317PurposedCas9, MCP-SoxS_R93ADepositorInsertdCas9 and MCP-SoxS_R93A
UseCRISPRExpressionBacterialMutationR93AAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-hOPTN
Plasmid#23053DepositorAvailable SinceMay 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pIntegrase_3
Plasmid#60575Purposeplasmid contains the integrase gene under the control of the arabinose-inducible promoter, PbadDepositorInsertIntegrase_3
ExpressionBacterialMutationCodon optimized for E.coliPromoterpBADAvailable SinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
p2attB-FST344-HA-CPL
Plasmid#131460PurposeEncodes human Follistatin proteinDepositorAvailable SinceMay 19, 2022AvailabilityAcademic Institutions and Nonprofits only