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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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34,238 results

Showing: 6981 - 7000 of 34238 results

  8. p28BIOHTEV-LIC

    Plasmid
    #167852
    Purpose
    Bacterial expression vector with N-terminal AviTag and C-terminal 6X-his tag for protein expression and purification
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Tags
    6xHis and AviTag
    Expression
    Bacterial
    Available since
    April 22, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    167852-plasmid-map-sequence-id-331065

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  9. t1-435

    Plasmid
    #166578
    Purpose
    For expression of human talin head (residues 1-435) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.
    Depositor
    Vesa Hytönen
    Publication
    Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11
    Insert
    Tln1 Head (1-435) (TLN1 Human)
    Tags
    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…
    Expression
    Bacterial
    Mutation
    residues 1-435 only
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166578-plasmid-map-sequence-id-330255

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  10. pBbB8k-csg-amylase

    Plasmid
    #166859
    Purpose
    Arabinose-inducible csgBACEFG operon for curli fiber synthesis in which curli subunit CsgA has been fused to α-amylase via a flexible linker. Derived from https://www.addgene.org/35363.
    Depositor
    Neel Joshi
    Publication
    Birnbaum et al Adv Sci (Weinh). 2021 May 3;8(11):200469
    Insert
    csgBACEFG, with α-amylase fused to CsgA via flexible linker
    Use
    Synthetic Biology
    Expression
    Bacterial
    Available since
    April 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    166859-plasmid-map-sequence-id-329647

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  11. Q-IL6-9

    Plasmid
    #166552
    Purpose
    scFv of a human scaffold targeting Interleukin-6. Antigen coverage aa 28-212 of 212, full-length excl. signal peptide
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166552-plasmid-map-sequence-id-328267

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  12. KG#908

    Plasmid
    #110914
    Purpose
    Expresses the plant proteins TIR1 (necessary for Auxin-Inducible Degradation) pan-neuronally in C. elegans
    Depositor
    Kenneth Miller
    Inserts
    rab-3 promoter
    TIR1 cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    Expression
    Bacterial
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    110914-plasmid-map-sequence-id-212553

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  13. pGEX4T-3-6AOptParkin

    Plasmid
    #165107
    Purpose
    bacterial expression of a GST fusion of e.coli optimized Parkin with 6 alanine linker between the GST and the inserted Parkin gene
    Depositor
    Arthur Haas
    Insert
    Parkin isoform 1 (PRKN Human)
    Tags
    GST-6-alanine
    Expression
    Bacterial
    Mutation
    codon optimized for E. coli expression
    Available since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    165107-plasmid-map-sequence-id-326710

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  18. pExp-MsyB

    Plasmid
    #112570
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MsyB (acidic protein MsyB from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Available since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    112570-plasmid-map-sequence-id-255699

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  20. pExp-mCherry

    Plasmid
    #112569
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8xHis, Strep II, and mCherry
    Expression
    Bacterial
    Available since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    112569-plasmid-map-sequence-id-217213

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Showing: 6981 - 7000 of 34238 results