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pET51b(+)_SNAP-p24

Plasmid

#130718

Purpose

Plasmid encoding the bacterial expression of a SNAP-fusion of p24 HIV Capsid protein

Depositor

Kai Johnsson

Publication

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

SNAP-Tag-p24

Tags

Hisx10 and StrepTag

Expression

Bacterial

Mutation

Deleted first Met, Ala and Last Ser from the Enha…

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCPP5912

Plasmid

#128703

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) and chaperone Gateway donor clone

Depositor

Alan Collmer

Publication

Kvitko et al PLoS Pathog. 2009 Apr;5(4):e1000388. doi

Insert

shcE-avrE1

Expression

Bacterial

Mutation

Three synonymous mutations Ala 362 (GCT-GCC), Ala…

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pSG019

Plasmid

#126502

Purpose

Pseudomonas aeruginosa disaggregase ClpG cloned as ClpG-6xHis

Depositor

Ute Romling

Publication

Lee et al Proc Natl Acad Sci U S A. 2018 Jan 9;115

Insert

disaggregase ClpG with 6xHis-tag

Tags

C-terminal 6xHis

Expression

Bacterial

Promoter

PBAD

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP3373

Plasmid

#128718

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Publication

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopQ1-1

Expression

Bacterial

Mutation

Synonymous point mutation in the wobble position…

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5238

Plasmid

#128711

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Publication

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopG1

Expression

Bacterial

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5052

Plasmid

#128729

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Publication

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopAM1

Expression

Bacterial

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP3416

Plasmid

#128720

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Publication

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopT1-1

Expression

Bacterial

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCPP5534

Plasmid

#128723

Purpose

Phytopathogen Pseudomonas syringae pv. tomato type III secretion system effector (gene lacking stop codon) Gateway donor clone

Depositor

Alan Collmer

Publication

Munkvold et al Mol Plant Microbe Interact. 2008 Apr;21(

Insert

hopX1

Expression

Bacterial

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pET-His6-Sumo-MBP-CfaN

Plasmid

#126016

Purpose

Bacterial expression of MBP-CfaN fusion protein (with N-terminal His5-Sumo)

Depositor

Thomas Muir

Insert

His5-Sumo-MBP-CfaN

Tags

5xHis-SUMO

Expression

Bacterial

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP23-1A(sfGFP)

Plasmid

#126970

Purpose

Level 1 vector. sfGFP reporter. Origin pBBR1 (medium-copy; broad-host-range)

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pFPV25.1_rpsM_mCerulean3

Plasmid

#124904

Purpose

rpsM promoter (S. typhimurium) driving mCerulean3 (codon-optimized for S. typhimurium)

Depositor

Markus Covert

Publication

Lane et al Cell Syst. 2019 Mar 27;8(3):183-196.e4.

Insert

rpsM promoter and mCerulean3

Expression

Bacterial

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pSUMO-LIC-CHIKV-nsP2h

Plasmid

#127935

Purpose

Expresses CHIKV nsP2h (1-465 aa) in bacterial cells

Depositor

Dahai Luo

Publication

Law et al Proc Natl Acad Sci U S A. 2019 May 7;116

Insert

CHIKV nsP2h

Tags

His6-Sumo-TEV

Expression

Bacterial

Promoter

T7 promoter

Available since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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tIVR

Plasmid

#122663

Purpose

Express tIVR protein in E. coli

Depositor

Robert Cohen

Publication

Choi et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

Insert

tIVR

Tags

His-tag

Expression

Bacterial

Promoter

T7 promoter

Available since

July 17, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pET-Sac-Abeta(MC1-42)

Plasmid

#127151

Purpose

Expresses N-terminal cysteine Abeta(1-42)

Depositor

James Nowick

Publication

Zhang et al Biochemistry. 2021 Apr 20;60(15):1191-12

Insert

Abeta(MC1-42) (APP Human)

Expression

Bacterial

Promoter

T7

Available since

July 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP42x-2A(sfGFP)

Plasmid

#126986

Purpose

Level 2 vector. sfGFP reporter. Origin RK2 (broad-host-range). CONTAINS one BsmBI and multiple BtgZI and AarI forbidden sites.

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pJUMP28-1A(sfGFP)

Plasmid

#126976

Purpose

Level 1 vector. sfGFP reporter. Origin pUC (high copy number)

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_WTDHFR_WTTS

Plasmid

#91226

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Publication

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET51b(+)-SNAP-CLIP

Plasmid

#127180

Purpose

Bacterial expression of the fused self-labeling tags SNAP-tag and CLIP-tag

Depositor

Kai Johnsson

Publication

Scarabelli et al ACS Sens. 2017 Aug 25;2(8):1191-1197. do

Insert

SNAP-CLIP

Tags

HisTag and StrepTag

Expression

Bacterial

Promoter

T7

Available since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pXWJ109As(RS-te-AAV)

Plasmid

#123156

Purpose

For TEV-based leakiness regulation. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t

Depositor

Baojun Wang

Publication

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-te-AAV-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J109, ParsR, PhrpLE

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pXW117As(RS-E11-RinA)2

Plasmid

#123146

Purpose

Arsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t

Depositor

Baojun Wang

Publication

Wan et al Nat Chem Biol. 2019 May;15(5):540-548. d

Insert

J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t

Use

Synthetic Biology

Expression

Bacterial

Promoter

J117, ParsR, PhrpLE, Pecf11

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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