Addgene

Showing: 7101 - 7120 of 29566 results

Results per page:

Sort results by:

BASIC_SEVA_66.11

Plasmid

#194213

Purpose

BASIC DNA assembly vector

Depositor

Paul Freemont

Article

Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

Insert

mScarlet counter-selection cassette

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2023

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pHH0103_NR4A3_Hormone-recep

Plasmid

#109952

Purpose

Protein expression and purification of NR4A3_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR4A3_Hormone-recep

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_RORB_Hormone-recep

Plasmid

#109958

Purpose

Protein expression and purification of RORB_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RORB_Hormone-recep

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_HNF4G_Hormone-recep

Plasmid

#109916

Purpose

Protein expression and purification of HNF4G_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

HNF4G_Hormone-recep (HNF4G Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAN-PA4-RFP

Plasmid

#62250

Purpose

reporter plasmid that encodes one of five synthetic sgRNA-repressible promoters that express mRFP1

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Insert

mRFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

pA4

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGBW-m4046814

Plasmid

#145601

Purpose

Bacterial Expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Benjie Chen, Ginkgo Bioworks

Insert

SARS-CoV-2 E (envelope) (E Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Cleavable TEV;6xHIS

Expression

Bacterial

Mutation

Escherichia coli recode 1

Promoter

Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

Available since

April 28, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
R989-X02-566: His6-MBP-tev-G-Hs.KRAS4b(1-169)

Plasmid

#159539

Purpose

E. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-169)

Depositor

Dominic Esposito

Article

Gillette et al Sci Rep. 2015 Nov 2;5:15916. doi: 10.103

Insert

His6-MBP-tev-G-Hs.KRAS4b(1-169) (KRAS Human)

Use

Tags

His6-MBP-tev

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
His6-Twin-Strep-SUMO-pET28a-EfaCas2

Plasmid

#112794

Purpose

To express enterococcus faecalis Cas2 protein in E. coli

Depositor

Ailong Ke

Article

Xiao et al Nature. 2017 Oct 5;550(7674):137-141. do

Insert

EfaCas2

Use

Tags

His6-Twin-Strep-SUMO

Expression

Bacterial

Mutation

Promoter

Available since

July 20, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDSG289

Plasmid

#115608

Purpose

surface-displayed Nb2, used in composability experiments; low copy

Depositor

Ingmar Riedel-Kruse

Article

Glass et al Cell. 2018 Jul 26;174(3):649-658.e16. do

Insert

regulated adhesin construct

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pJMP1189

Plasmid

#119257

Purpose

rfp "test" strain

Depositor

Jason Peters, Carol Gross, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Use

Tags

Expression

Bacterial

Mutation

D10A and H840A

Promoter

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEB1-T-Sapphire

Plasmid

#103977

Purpose

Plasmid encoding T-Sapphire

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

T-Sapphire

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (Q69M, C70V, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
Lapronic/pRSETB

Plasmid

#140755

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Lapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pETDuet RagA(D130N)-RagC

Plasmid

#99642

Purpose

Bacterial expression of RagA(D130N)-RagC

Depositor

David Sabatini, Kuang Shen

Article

Shen et al Mol Cell. 2017 Oct 17. pii: S1097-2765(1

Inserts

RagA (RRAGA Human)
RagC (RRAGC Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

D130N

Promoter

Available since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pKS101

Plasmid

#24631

Depositor

Jay Keasling

Article

Steen et al Nature. 2010 Jan 28;463(7280):559-62.

Insert

FAA2 (FAA2 Budding Yeast)

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pMD18-T-pyrF

Plasmid

#174388

Purpose

modified pMD18-T containing pyrF and its native promoter, Ampr

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

pyrF

Use

Template for cloning fragments for chromosomal in…

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHLUK

Plasmid

#64181

Purpose

harbours marker genes HIS3, LEU2, URA3 and LYS2

Depositor

Markus Ralser

Article

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS hBAF53a

Plasmid

#17879

Depositor

Jerry Crabtree

Article

Zhao et al Cell. 1998 Nov 25. 95(5):625-36.

Insert

BAF53a (ACTL6A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only
pELlR1

Plasmid

#172569

Purpose

Inducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Chloramphenicol Resistance Marker.

Depositor

Christopher Reisch

Insert

LacI-mRFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLlacO-1

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pELlR2

Plasmid

#172555

Purpose

Inducible gene expression, pET origin, LacI repressor, PLlacO-1 promoter, mRFP, Kanamycin Resistance Marker.

Depositor

Christopher Reisch

Insert

LacI-mRFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PLlacO-1

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJSC129 - Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

Plasmid

#101211

Purpose

Bacterial expression plasmid for SpCas9-HF1, REC2 FRET variant

Depositor

Jennifer Doudna, Keith Joung

Article

Chen et al Nature. 2017 Sep 20. doi: 10.1038/nature

Insert

SpCas9 variant C80S/C574S/E60C/D273C/N497A/R661A/Q695A/Q926A

Use

Tags

10x His, MBP, and TEV site

Expression

Bacterial

Mutation

C80S, C574S, E60C, D273C, N497A, R661A, Q695A and…

Promoter

T7

Available since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge