32,453 results
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pET151-GyrB
Plasmid#216233PurposeS. aureus USA300 GyrB Codon-optimized for E. coliDepositorInsertDNA gyrase
Tags6xHisExpressionBacterialPromoterT7Available SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
Yellow Cameleon-Nano15/pBig
Plasmid#51962PurposeExpression of Yellow Cameleon-Nano15, an ultrasensitive Ca2+ indicator with Kd = 15 nM, in DictyosteliumDepositorInsertYellow Cameleon-Nano15
ExpressionBacterialAvailable SinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC-UAS1B16-TEF(504)
Plasmid#44371DepositorInsertUAS1B16-TEF(504) promoter
UseSynthetic BiologyExpressionBacterialMutationN/APromoterUAS1B16-TEF(504)Available SinceJuly 15, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
p15A-OptoCre-knt-P*-R
Plasmid#189850PurposeCre-inducible activation of knt for kanamycin resistance.DepositorInsertP*-loxP-TT-loxP-R-knt
UseCre/Lox and Synthetic BiologyExpressionBacterialPromoterP* (modified T7 phage gene 10)Available SinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
p12x601
Plasmid#157785PurposeEcoRV digestion produces 12x601 chromatin assembly construct with central TetO following PEG removal of plasmid backboneDepositorInsertpMD2.G(spei/spei)&12x601_tetO
ExpressionBacterialAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pE1n
Plasmid#17441Purposemodified pENTR vector for N-terminal STREP tag fusion with your gene of interestDepositorTypeEmpty backboneUseModified gateway entry vectorTagsSTREPExpressionBacterial, Insect, Mammalia…Available SinceMarch 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6P-1 Stu2 1-306
Plasmid#38315DepositorAvailable SinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Bacteroidetes-bGSDM-TEV
Plasmid#222101PurposeFor expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.DepositorInsertBacteroidetes-bGSDM-TEV ORF
Tags6xHis-SUMO2ExpressionBacterialMutationTEV protease site replaces residues E242–G248PromoterT7Available SinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTU1-B-SP44s-GUS
Plasmid#172430PurposeExpresses Streptomyces codon-optimised beta-D-glucuronidaseDepositorInsertbeta-D-glucuronidase
UseSynthetic BiologyTagsNoneExpressionBacterialPromoterSP44sAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDD04neo pmyo2::gfp
Plasmid#26393DepositorTypeEmpty backboneExpressionBacterial and WormAvailable SinceOct. 22, 2010AvailabilityAcademic Institutions and Nonprofits only -
pSR1051
Plasmid#208739PurposeCharacterization plasmid for PZwf (sfgfp expressed from PZwf on the S. aureus pC194 replicon)DepositorInsertPZwf
UseSynthetic BiologyExpressionBacterialPromoterPZwfAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSR1057
Plasmid#208742PurposeCharacterization plasmid for PPgk (sfgfp expressed from PPgk on the S. aureus pC194 replicon)DepositorInsertPPgk
UseSynthetic BiologyExpressionBacterialPromoterPPgkAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM33_CRK_SH3
Plasmid#178472PurposeBacterial expression of human domain with His-tag and GST tagDepositorAvailable SinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
GST-PS-Gads 1-155 GB1 Gads 261-330-Cys (XSB365)
Plasmid#78507PurposeConstruct for expressing Gads SH3, SH2, and SH3 domain in E. Coli, with a C-terminal Cys for labelingDepositorInsertGads SH3 SH2 SH3 domain (GRAP2 Human)
TagsGST followed by a prescission protease claevage s…ExpressionBacterialAvailable SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-jREX-GECO1
Plasmid#113941PurposeBacterial expression of red fluorescent Ca2+ sensor jREX-GECO1DepositorInsertjREX-GECO1
Tags6xHis and Modified TorA MNNNDLFQASRRRFLAQLG[G to …ExpressionBacterialAvailable SinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLsCas13a-gRNA-NT
Plasmid#164857PurposeConstitutive expression of single-spacer CRISPR array with non-targeting spacer for LsCas13a in bacteria.DepositorInsertLsCas13a-gRNA-NT
ExpressionBacterialPromoterJ23119Available SinceFeb. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACT2-CAS9c
Plasmid#51055PurposeExpress Eukaryotic-codon-optimized Cas9c gene in yeast under ADH1 promoter for genome editing. SV40 NLS is fused to the C terminal. GAL4 region from pACT2 is removed.DepositorInsertCas9c
UseCRISPRTagsSV40 NLSExpressionBacterial and YeastPromoteryeast ADH1 promoterAvailable SinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGCP123-GFP_g1
Plasmid#153518PurposesgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcodedDepositorInsertPnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
6798 pJC25NMstop
Plasmid#1228DepositorInsertNM (SUP35 Budding Yeast)
ExpressionBacterialAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only
