32,218 results
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Plasmid#35333DepositorInsertRFP
UseSynthetic BiologyExpressionBacterialPromoterTrcAvailable SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDB036d
Plasmid#99216PurposeAccessory plasmid for PACE of MjTyrRS variants; negative selectionDepositorInsertPpsp [SD8] gIII; PproK tyrT(Opt,CUA); PproA [SD4] T7RNAP(S12*,S203*)
ExpressionBacterialMutationamber codons at positions 12 and 203 of T7 RNA po…PromoterPpsp, PproK, and PproAAvailable SinceNov. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pExp-RBD-CHis
Plasmid#195002PurposeProduction of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tagDepositorAvailable SinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCoofy33
Plasmid#44001DepositorTypeEmpty backboneTagsHis10 and OneStrepExpressionBacterialPromoterT7Available SinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits only -
pQLinkHD-SYNZIP4
Plasmid#80660PurposeEncodes the synthetic coiled coil peptide SYNZIP4DepositorInsertSYNZIP4
Tags6xHisExpressionBacterialAvailable SinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFVR5
Plasmid#149489PurposeBroad-Host range expression vector, RSF1010 origin, VanR(AM) repressor, PVanCC promoter, mRFP, Kanamycin Resistance Marker.DepositorInsertVanR(AM)-mRFP
UseSynthetic BiologyExpressionBacterialPromoterPVanCCAvailable SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX Slp76-SH2
Plasmid#46503DepositorInsertSlp76 (LCP2 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 417-533PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX CrkL-SH2
Plasmid#46419DepositorInsertCrkl (CRKL Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 9-108PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pColdII-SIRPT1-Loxodonta Africana
Plasmid#75351PurposeNt His-tag Loxodonta Africana 89-1368DepositorInsertSacsin Loxodonta Africana 89-1368
TagsHis-TagExpressionBacterialAvailable SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-17
Plasmid#172747PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-17; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-17
Tags6xHIS and pelB leader for periplasmic secretionExpressionBacterialPromoterT7Available SinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJMP2774
Plasmid#160667PurposeMobile CRISPRi sfGFP 'test', gmc6 sgRNADepositorInsertgmc6 (gfp) sgRNA
ExpressionBacterialAvailable SinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOGG004
Plasmid#113979PurposeVector construction module. Level 1 golden gate cloning site module with LacZ reporterDepositorInsertLevel 1 golden gate cloning site
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceJan. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp15-DARPinOFF7
Plasmid#182399PurposeBacterial expression of Vesicle Nucleating peptide15-DARPinOFF7 fusionDepositorInsertVNp15-DARPinOFF7
TagsVesicle Nucleating peptide 15 (VNp15)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSkunk3-BLA
Plasmid#61531PurposePhagemid expressing streptomycin/spectinomycin resistance geneDepositorInsertaadA
ExpressionBacterialPromoterlacIAvailable SinceDec. 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
pZH520
Plasmid#102673PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-XIAP1-351
Plasmid#8342DepositorAvailable SinceApril 20, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET29-hMIGA2(170-593)-6xhis
Plasmid#192834PurposeExpress codon-optimized human MIGA2 fragment 170-593 with a C-terminal 6xhis tag (lipid transfer construct) in bacteriaDepositorInsertCodon-optimized human Mitoguardin 2 (MIGA2 Human)
Tags6xhisExpressionBacterialPromoterT7Available SinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLN
Plasmid#40028DepositorInsertsloxP
Neo
ExpressionBacterial and MammalianPromoterNone and SV40Available SinceDec. 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPath-pR-integrase
Plasmid#200000PurposeEntry vector for biosynthetic pathway. pR-phiC31 integraseDepositorInsertPathways
ExpressionBacterialAvailable SinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM-hu FceRI beta
Plasmid#8366DepositorInserthuman Fc epsilon RI beta cDNA (MS4A2 Human)
ExpressionBacterialAvailable SinceJune 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAV124
Plasmid#63190PurposepRS424 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterial and YeastAvailable SinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109HgAmp30E
Plasmid#123149PurposeMercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, PmerT, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
HERC2 (3KCI)
Plasmid#25344PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertherc2.3951.4321 (HERC2 Human)
TagsHisExpressionBacterialMutationcontains amino acid residues 3951-4321Available SinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRSET-pr-mKikGR1 (mKikGR1-V69T)
Plasmid#99227PurposeBacterial expression of the primed conversion capable protein pr-mKikGR1 (mKikGR1-V69T).DepositorInsertpr-mKikGR1
ExpressionBacterialMutationmonomeric variant with mutation V69T (Eos notatio…PromoterT7Available SinceApril 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBM3R1-B1
Plasmid#74678PurposeBM3R1-B1 gateDepositorInsertPTac-RBSB1-BM3R1-PBM3R1-YFP
ExpressionBacterialAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
His-PHD2-181-426
Plasmid#223551PurposeHis-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purificationDepositorInsertPHD2 (EGLN1 Human)
Tags6x HisExpressionBacterialMutationDeleted amino acids 1-180.PromotertrcAvailable SinceSept. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28a_PA4114_Cterm_His
Plasmid#220484Purposeheterologous expression of PA4114 for protein purificationDepositorInsertPA4114
Tags6xHsExpressionBacterialMutationnoneAvailable SinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
HSP90B1
Plasmid#39076PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMay 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pNSSCPG
Plasmid#183187PurposePACE-evolved split N (core) terminal of T7 RNA polymerase-SpmXΔC and SpmXΔC-PACE-evolved split C (sigma) terminal of T7 RNA polymerase both expressed under Tac promoter; sfGFP-LAA expressed under T7 pDepositorInsertssuperfolder GFP
SpmX450 and T7 181+ fusion protein
T7 RNAP 1-179 variant and SpmX450 fusion protein
mRFP1 and popZ fusion protein
ExpressionBacterialAvailable SinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
B7-3
Plasmid#111491PurposeTyr-tRNA synthetaseDepositorInsertTyr-tRNA synthetase
Tags6xHisExpressionBacterialAvailable SinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only