32,218 results
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Plasmid#104439PurposeDestination vector expressing plant-codon-optimized Cas9 under 35S promoter, with sgRNAs be shuffled in; seed coat specific red fluorescence for screening trangene freeDepositorTypeEmpty backboneUseCRISPRExpressionBacterial and PlantPromoter35SAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only
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pET-22b(+)-HpENR
Plasmid#12651DepositorInsertnoyl-acyl carrier protein reductase (fabI Helicobacter pylori)
ExpressionBacterialAvailable SinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
pBFC0694
Plasmid#186693PurposeShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_lacZ_α_1 gRNA, GmR+sfGFP cargoDepositorInsertstnsB
tnsC
tniQ
Cas12k
Sh_lacZ_α_1 gRNA
Tn7 transposon
GmR+sfGFP ShDART Cargo
ExpressionBacterialPromoterPLacAvailable SinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAB082b
Plasmid#79234PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPpro1 rpoZ-Cry1Ac-d3
ExpressionBacterialPromoterPpro1Available SinceDec. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZH521
Plasmid#102674PurposeTetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assemblyDepositorInsertGFPmut2
ExpressionBacterialAvailable SinceDec. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBbB1k-GFP
Plasmid#35342DepositorInsertGFP
UseSynthetic BiologyExpressionBacterialPromoterTrcAvailable SinceFeb. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET29b-mVenus-dspB(E184Q)-6xHis
Plasmid#176576PurposePlasmid for overexpression of recombinant His-tagged fusion protein mVenus-DspB(E184Q), used as a fluorescent probe for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
TagsHexahistidine tag and mVenusExpressionBacterialMutationE184QPromoterT7Available SinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-RARS-5
Plasmid#166535PurposescFv of a human scaffold targeting Arginyl-tRNA synthetase. Antigen coverage aa 70-660 of 660DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-KARS-5
Plasmid#166530PurposescFv of a human scaffold targeting Lysyl-tRNA synthetase. Antigen coverage aa 1-597 of 597, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-DARS-1
Plasmid#166538PurposescFv of a human scaffold targeting Aspartyl-tRNA synthetase. Antigen coverage aa 1-501 of 501, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
O-LARS-15
Plasmid#166542PurposescFv of a human scaffold targeting Leucyl-tRNA synthetase. Antigen coverage aa 260-509 of 1176DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialPromoterLacZAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
KIF2C
Plasmid#25551PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 25, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pXD70CT5-PxylR-CymR, Pveg(CuO)-LacZ
Plasmid#191627PurposeE. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5 (engineered from pCT5-bac2.0)-LacZDepositorInsertlacZ
ExpressionBacterialAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
SETD2
Plasmid#25348PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 17, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
HOXC9 (murine) HIS-tag pET
Plasmid#8559DepositorAvailable SinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
RAB9B
Plasmid#25625PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceAug. 30, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pet15_rbsk
Plasmid#220227PurposeBacterial expression of Rbsk in E. coli BL21_DE3 (pLys) or similarDepositorAvailable SinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET3C-ARL16(CO)-His6
Plasmid#216485PurposeUsed for expression of human ARL16, short form, in bacteria. The open reading frame has been codon optimized for bacterial expression and contains a glycine rich linker and His6 tag at the C-terminus.DepositorInsertADP-ribosylation factor-like 16 (ARL16 Human)
Tagsincludes GSGSGSHHHHHH (His6) tag at C-terminusExpressionBacterialPromoterT7Available SinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-CV-Ykt6
Plasmid#168030PurposeExpresses eCFP-Ykt6(Q131-K190)-mVenus in bacterial cellsDepositorInserteCFP-Ykt6(Q131-K190)-mVenus
TagsHisx6ExpressionBacterialAvailable SinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
B6-1
Plasmid#111485PurposePhe-tRNA synthetase BDepositorInsertPhe-tRNA synthetase B
Tags6xHisExpressionBacterialAvailable SinceSept. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
sfTq2-PBP5
Plasmid#117959PurposePeriplasmic expression of superfolder mTurquoise2 (pNM067 pTHV037-DsbAss-LEGPAGL-sfTq2-EFGS-∆1-17-PBP5)DepositorInsertsfTq2-PBP5 (dacA )
ExpressionBacterialAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
5787 pET28aSX104B/ pES42
Plasmid#1229DepositorAvailable SinceJan. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
pSrpR-S4
Plasmid#74694PurposeSrpR-S4 gateDepositorInsertPTac-RBSS4-SrpR-PSrpR-YFP
ExpressionBacterialAvailable SinceApril 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBM3R1-B3
Plasmid#74680PurposeBM3R1-B3 gateDepositorInsertPTac-RBSB3-BM3R1-PBM3R1-YFP
ExpressionBacterialAvailable SinceApril 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDJSVT89
Plasmid#109303PurposeExpress periplasmic, outer membrane, or secreted proteins in E. coli - TamA signal and His6 tagDepositorTypeEmpty backboneTagsTamA 1-27, 6HisExpressionBacterialPromoterT7Available SinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
MBP-hnRNPA2_LC
Plasmid#98661PurposeExpresses MBP-tagged hnRNPA2 AA 190-341DepositorAvailable SinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBPHLWL-GAL4DBD
Plasmid#26256DepositorInsertZIP- Gal4DBD (GAL4 Budding Yeast)
UseDrosophila transgenesisExpressionBacterial and InsectAvailable SinceFeb. 2, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBluescript goosecoid
Plasmid#16935DepositorInsertgoosecoid (gsc Zebrafish)
ExpressionBacterialAvailable SinceMay 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pALS343 (pGEX-2TP-cTevHis-Gibson/MCS)
Plasmid#139788PurposeVector for recombinant protein expression in E. coli; PreScission-cleavable N-terminal GST and TEV-cleavable C-terminal 6xHisDepositorTypeEmpty backboneTags6xHis (cleavable by TEV protease) and GST (cleava…ExpressionBacterialAvailable SinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3b_ADRP
Plasmid#156468PurposeBacterial expression of Sars-CoV2 Nsp3b_ADRP protein with His-tag and GST-tagDepositorInsertNsp3b_ADRP (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only