30,844 results

1. Jnk3a1

   Plasmid

   #29577

   Depositor

   Dustin Maly

   Article

   Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

   Insert

   Jnk3a1 (MAPK10 Human)
   

   Tags

   HIS6 and TEV

   Expression

   Bacterial

   Mutation

   Contains residues 40-402

   Available Since

   July 16, 2011

   Availability

   Academic Institutions and Nonprofits only

2. 819 pGEX2T PTEN C124S

   Plasmid

   #10745

   Depositor

   William Sellers

   Article

   Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

   Insert

   PTEN (PTEN Human)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C124S

   Available Since

   Sept. 27, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pTorPE-G-GECO1

   Plasmid

   #32466

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   G-GECO1.0
   

   Use

   Lab constructed

   Tags

   6 His tag and Modified TorA MNNNDLFQASRRRFQAQLGGL…

   Expression

   Bacterial

   Mutation

   GCaMP3 K119I/L173Q/S404G/E430V

   Available Since

   Sept. 30, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRKO

   Plasmid

   #49456

   Purpose

   loxP-kanMX-loxP cassette inserted into the ACT1 intron for PCR amplification of the intron/kanMX module for one-step homology-driven genomic disruptions

   Depositor

   Marc Gartenberg

   Article

   Cheng et al Nucleic Acids Res. 2000 Dec 15;28(24):E1

   Insert

   loxP-kanMX-loxP cassette
   

   Use

   Cre/Lox

   Expression

   Bacterial

   Promoter

   none

   Available Since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. MKK2

   Plasmid

   #29580

   Depositor

   Dustin Maly

   Article

   Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

   Insert

   MKK2 (MAP2K2 Human)
   

   Tags

   GST, HIS6, and TEV

   Expression

   Bacterial

   Mutation

   Constitutively active mutant; Starting Met remove…

   Available Since

   June 7, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pET23a-His-hRanGAP1tail

   Plasmid

   #53139

   Purpose

   E.coli expression of Cterminal domain of human RanGAP1 with Nterminal His-tag

   Depositor

   Frauke Melchior

   Article

   Flotho et al Methods Mol Biol. 2012;832:93-110. doi:

   Insert

   RanGAP1 (RANGAP1 Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   C-terminal fragment (amino acids 398-587)

   Promoter

   T7

   Available Since

   May 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBAD/HisB-iRFP682

   Plasmid

   #45454

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

   Insert

   iRFP682
   

   Expression

   Bacterial

   Mutation

   T2A, S13L, A92T, V104T, V114I, E161K, E180V, Y193…

   Promoter

   araBAD

   Available Since

   June 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET23a-HsRPS27a

   Plasmid

   #69561

   Purpose

   Bacterial expression; in vitro transcription/translation (T7)

   Depositor

   Jorge Eduardo Azevedo

   Article

   Grou et al Sci Rep. 2015 Aug 3;5:12836. doi: 10.103

   Insert

   RPS27A (RPS27A Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

9. pLP-pR-himar-apraR

   Plasmid

   #199996

   Purpose

   Landing Pad with T7 RNAP circuit variant T15. pC194+Tn903 seed promoter. pR-himar transposase. In dap auxotrophic conjugation donor

   Depositor

   Farren Isaacs

   Article

   Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

   Insert

   Landing Pads
   

   Expression

   Bacterial

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBTBXh-2

    Plasmid

    #26078

    Depositor

    Ryan Gill

    Article

    Prior et al Biotechnol Bioeng. 2010 Jun 1. 106(2):32

    Type

    Empty backbone

    Tags

    His

    Expression

    Bacterial

    Available Since

    Nov. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBSFlp

    Plasmid

    #45470

    Depositor

    Michele Swanson

    Article

    Bryan et al Appl Environ Microbiol. 2011 Apr;77(7):2

    Insert

    Flp recombinase
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET-45b-mCherry

    Plasmid

    #145279

    Purpose

    Bacterial Expression of 6xHis mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    mCherry
    

    Expression

    Bacterial

    Available Since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28CLY5

    Plasmid

    #21764

    Depositor

    Maarten Merkx

    Article

    Evers et al Biochemistry. 2006 Nov 7. 45(44):13183-9

    Insert

    ECFP -EYFP linked by flexible linker containing 5 SGGSGG repeats
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pQmod2E-GG

    Plasmid

    #191345

    Purpose

    Clostridium expression vector (pBP1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sites

    Depositor

    Andrew Tolonen

    Article

    Rostain et al ACS Synth Biol. 2022 Dec 16;11(12):4077-

    Insert

    Plac-RFP (IPTG-inducible red fluorescent protein)
    

    Expression

    Bacterial

    Promoter

    Plac

    Available Since

    Nov. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCW17 - pAdapter

    Plasmid

    #174664

    Purpose

    Constitutive expression of λCI fused via three alanines to the MS2 coat protein. Useful as RNA-DNA adapter in three-hybrid system.

    Depositor

    Katherine Berry

    Article

    Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507

    Insert

    MS2 coat protein
    

    Tags

    λCI

    Expression

    Bacterial

    Mutation

    V30I, A81G, positions 68-80 deleted (VATQTVGGVELP…

    Promoter

    Synthetic Constitutive Promoter

    Available Since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3xFLAG-CUL7-pCMV7.1

    Plasmid

    #155025

    Purpose

    N-terminal 3xFLAG-tagged CUL7 in a pCMV7.1 vector

    Depositor

    Andre Catic

    Article

    Sweeney et al Sci Rep. 2020 Aug 18;10(1):13942. doi: 1

    Insert

    Cullin 7 (CUL7 Human)
    

    Tags

    3xFLAG

    Expression

    Bacterial

    Promoter

    pCMV7.1

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pV1F

    Plasmid

    #205084

    Purpose

    Prokaryotic expressing of AtVIP1 fused with a sequence encoding a T4SS translocation tag in A. tumefaciens.

    Depositor

    Suomeng Dong

    Article

    Wang et al PLoS Pathog. 2023 Apr 21;19(4):e1011346.

    Insert

    AtVIP1
    

    Expression

    Bacterial

    Promoter

    agorbacterium virB operon promoter

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pQLinkHD_ECFP

    Plasmid

    #118857

    Purpose

    fluorescent protein expression with an N-terminal His-tag

    Depositor

    Marc Birtwistle

    Article

    Holzapfel et al ACS Comb Sci. 2018 Nov 12;20(11):653-659

    Insert

    ECFP
    

    Tags

    His-tag

    Expression

    Bacterial

    Available Since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pQTEV-CDO1

    Plasmid

    #31292

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

    Insert

    cysteine dioxygenase, type I (CDO1 Human)
    

    Tags

    His and TEV

    Expression

    Bacterial

    Available Since

    Nov. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST-HemmarR2

    Plasmid

    #112813

    Purpose

    gateway cloning destination vector for expressing enhancer driven CD4-tdTomato

    Depositor

    Chun Han

    Article

    Poe et al Genetics. 2018 Nov 30. pii: genetics.118

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    June 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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