30,176 results

1. pET-Duet1_6xHis-TEV-GABARAP-Gly

   Plasmid

   #190930

   Purpose

   Plasmid for the expression and purification of GABARAP. Internal Reference: SMC894

   Depositor

   Sascha Martens

   Article

   Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

   Insert

   GABARAP (GABARAP Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Ends with Glycine 116

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-CohIII-CBM-HIS-LPETGG

   Plasmid

   #91697

   Purpose

   CohIII-CBM handle with C-Terminal Sortase Recognition Sequence LPETGG for Posttranslational Coupling to a N-terminal GGG

   Depositor

   Hermann Gaub

   Article

   Ott et al ACS Nano. 2017 Jun 12. doi: 10.1021/acsn

   Insert

   CohIII-CBM-HIS-LPETGG
   

   Use

   Tags

   HIS-Tag and Sortase-Tag

   Expression

   Bacterial

   Mutation

   Cysteine deletion in CBM C63S

   Promoter

   T7

   Available since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSETB-de4GFP

   Plasmid

   #85472

   Purpose

   Inducible expression of dual emitting pH sensitive GFP in bacterial cells

   Depositor

   S. James Remington

   Article

   Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

   Insert

   de4GFP
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   S65T/C48S/H148C/T203C

   Promoter

   T7

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSWAP_Entry_H2_beta_GM

   Plasmid

   #184871

   Purpose

   pSwap plasmid part with H2_beta homology arms and gentamycin cassette

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. pE-SUMO CRYBB2

   Plasmid

   #80749

   Purpose

   Expression of human beta B2-crystallin in E. coli

   Depositor

   Larry David

   Insert

   CRYBB2 (CRYBB2 Human)
   

   Use

   Tags

   6xHIS and Smt3 SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGSTag Bmf

   Plasmid

   #24264

   Depositor

   Roger Davis

   Article

   Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

   Insert

   Bmf (BMF Human)
   

   Use

   Tags

   Gst

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 1, 2010

   Availability

   Academic Institutions and Nonprofits only

7. T4 Lysozyme S38D L99A M102Q N144D

   Plasmid

   #18477

   Depositor

   Brian Matthews

   Insert

   T4 Lysozyme (e Bacteriophage T4)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   S38D L99A M102Q N144D

   Promoter

   Available since

   Sept. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

8. pIA1400

   Plasmid

   #172519

   Purpose

   Expression of SARS-COV-2 Nsp12 in E. coli

   Depositor

   Irina Artsimovitch

   Article

   Wang et al Nucleic Acids Res. 2021 Aug 5. pii: 6342

   Insert

   SARS-COV-2 Nsp12 (ORF1ab Synthetic)
   

   Use

   Tags

   His-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21b-Caspase-3(D3A: D175A D28A D9A)

   Plasmid

   #90090

   Purpose

   Expresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cells

   Depositor

   Clay Clark

   Article

   Bose et al Biochemistry. 2003 Oct 28;42(42):12298-3

   Insert

   Caspase 3 (CASP3 Human)
   

   Use

   Tags

   His tag

   Expression

   Bacterial

   Mutation

   D175A D28A D9A

   Promoter

   Available since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. KE-009

    Plasmid

    #133441

    Purpose

    Human elF4H, residue 1-228 (full-length), N-terminal His, GB1, TEV; in pET21 backbone.

    Depositor

    Gerhard Wagner

    Insert

    EIF4H (EIF4H Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBBR1_DVK_AE(J23106-B0034m-E0040m-B0015)

    Plasmid

    #197671

    Purpose

    Expresses GFP under the control of J23106 and B0034m

    Depositor

    Eric Young

    Article

    Keating et al ACS Synth Biol. 2023 Jun 9. doi: 10.1021

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDest-521

    Plasmid

    #159688

    Purpose

    Gateway destination vector for E. coli protein expression

    Depositor

    Dominic Esposito

    Article

    Tran et al Nat Commun. 2021 Feb 19;12(1):1176. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHYP-NPC-10H

    Plasmid

    #162789

    Purpose

    SARS-CoV-2 Nucleocapsid Protein expression in E.coli

    Depositor

    Ivan Vorobiev

    Article

    Kolesov et al PeerJ. 2022 Jan 7;10:e12751. doi: 10.771

    Insert

    Nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7lac

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOPO717

    Plasmid

    #195302

    Purpose

    pBAD322-MycaIDCas-lacZsp5, arapBAD promoter expressing type I-D Cascade and one spacer array of McCAST (Tn7575), the single spacer is lacZ spacer 5.

    Depositor

    Joseph Peters

    Article

    Hsieh et al Nucleic Acids Res. 2023 Jan 25;51(2):765

    Insert

    McCAST Cascade and lacZ spacer 5 in native array
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Arabinose inducible arapBAD

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. RAP1B

    Plasmid

    #55665

    Purpose

    expression of mouse RAP1B

    Depositor

    Julian Downward

    Article

    Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

    Insert

    RAP1B (Rap1b Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pGBW-m4046955

    Plasmid

    #145616

    Purpose

    Bacterial Expression plasmid for RNA-dependent RNA polymerase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    RNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pLL2787

    Plasmid

    #15460

    Depositor

    Chia Lee

    Article

    Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

    Insert

    phage phi11 int gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDule2-3-nitroTyrosine (5B)

    Plasmid

    #85499

    Purpose

    Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.

    Depositor

    Ryan Mehl

    Article

    Cooley et al Biochemistry. 2014 Apr 1;53(12):1916-24.

    Insert

    3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32H H70C D158S I159A L162R

    Promoter

    lpp (constitutive)

    Available since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRSFDuet-1_VNp-LZ-mNeongreen

    Plasmid

    #182392

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-LZ-mNeongreen
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pExp-NHis

    Plasmid

    #112558

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Use

    Tags

    8xHis and Strep tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge