33,459 results
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Plasmid#149638Purposeparental, all-in-one CRISPRi vector for B. burgdorferi, emptyDepositorTypeEmpty backboneUseCRISPRExpressionBacterialPromoterPflaB, PpQE30Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only
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T7 promoter + rpsL reporter plasmid
Plasmid#156455PurposeA mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.DepositorInsertsrpsL
TcR
ExpressionBacterialMutationATG start codon mutated to ACGPromotertac and tetAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2834
Plasmid#160673PurposeMobile CRISPRi sfGFP 'test', gmc6 sgRNADepositorInsertgmc6 (gfp) sgRNA
ExpressionBacterialAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pJMP2822
Plasmid#160670PurposeMobile CRISPRi sfGFP 'test', empty sgRNADepositorInsertempty sgRNA (BsaI)
ExpressionBacterialAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTET-IUPi
Plasmid#122019PurposeExpresses IUP pathway genes (ChK, IPK, idi) under inducible promoter. aTC inducible. Kan. resist.DepositorInsertsExpressionBacterialMutationCodon optimized for E. coliPromoterpTETAvailable sinceOct. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
EcRho
Plasmid#113121PurposeExpresses E. coli RhoDepositorInsertRho
TagsMGHExpressionBacterialPromoterT7Available sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T1-Rpb4/7
Plasmid#138484PurposeExpresses GST-tagged Rpb4 and His-tagged Rpb7 in bacteriaDepositorTags6xHis and GSTExpressionBacterialPromotertacAvailable sinceOct. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
poRBS
Plasmid#154125PurposeExpresses orthogonal rRNAs operon in bacterial cellsDepositorInsertengineered bacterial rRNAs operon under control of pL promotor
UseSynthetic BiologyExpressionBacterialAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCri_L706-antiSH2-OptoMB
Plasmid#159935PurposeOpto-MB bacterial expressionDepositorInsertanti-SH2 MB with SS58 insert
ExpressionBacterialAvailable sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJDC60
Plasmid#157780PurposetdTomato is expressed under the mycobacterial L5 promoter for in vivo and in vitro detection.DepositorInserttdTomato
ExpressionBacterialPromoterL5Available sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
R989-X05-566: His6-MBP-tev-G-Hs.KRAS4b(1-188)
Plasmid#159546PurposeE. coli protein expression of His6-MBP-tev-G-Hs.KRAS4b(1-188)DepositorAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
R711-X12-566: His6-MBP-tev-Hs.NF1iso2 (1203-1530)
Plasmid#159580PurposeE. coli protein expression of His6-MBP-tev-Hs.NF1iso2 (1203-1530)DepositorAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
R701-X52-566: His6-MBP-tev-Avi-Hs.KRAS4b(2-169)
Plasmid#159560PurposeE. coli protein expression of His6-MBP-tev-Avi-Hs.KRAS4b(2-169)DepositorAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSEVA231-CRISPR
Plasmid#138712PurposePlasmid designed to clone a specific spacer into BsaI sites to generate a CRISPR ArrayDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionBacterialAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
EC10161
Plasmid#154060PurposeLevel 0.5 Golden Gate backbone, containing loxP sites.DepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyExpressionBacterialPromoterN/AAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-45b-RUNX2(WT)-IDR-mCherry
Plasmid#145286PurposeBacterial Expression of 6xHis RUNX2(WT) IDR mCherry Fusion ProteinDepositorInsertRUNX2(WT)-IDR (RUNX2 Human)
ExpressionBacterialAvailable sinceSept. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEST_OR2-OR1-Pr-UTR1_ClpP-T500
Plasmid#153302PurposeExpresses ClpP protease in the cell-free TX-TL systemDepositorInsertclpP (clpP Escherichia coli)
ExpressionBacterialAvailable sinceSept. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPfor
Plasmid#158059PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the forward orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyExpressionBacterialPromoteracpPAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
hnRNPA2_RRM1
Plasmid#157865Purposeexpress His tagged hnRNPA2 RRM1DepositorAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hnRNPA2_RRM2
Plasmid#157866Purposeexpress His tagged hnRNPA2 RRM2DepositorAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by