32,453 results

Showing: 7761 - 7780 of 32453 results

Results per page:

Sort results by:

pJL017

Plasmid

#53532

Purpose

This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

Depositor

James Fleckenstein

Article

Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

Insert

etpBA

Tags

6His and myc

Expression

Bacterial

Promoter

pBAD

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCORE-UH

Plasmid

#72239

Purpose

Contains the CORE cassette with KlURA3 and hyg markers

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Genet Eng (N Y). 2003;25:189-207.

Inserts

KlURA3
hyg

Expression

Bacterial

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a AHU

Plasmid

#26640

Depositor

Phoebe Rice

Article

Swinger et al EMBO J. 2003 Jul 15. 22(14):3749-60.

Insert

AHU

Expression

Bacterial

Available Since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pEK19

Plasmid

#210578

Purpose

Reporter gene expression in B. dendrobatidis

Depositor

Lillian Fritz-Laylin

Article

Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

Insert

BdBIP1pro-Hshph-G4S-nanoluc-ScADH1ter

Use

Luciferase

Tags

NanoLuc luciferase

Expression

Bacterial and Yeast

Promoter

BdBIP1pro

Available Since

Jan. 22, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
371-GFP

Plasmid

#97030

Purpose

His6-GFP-tev-yORF

Depositor

Chris Jeans

Type

Empty backbone

Tags

GFP fusion, His6 tag, and TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
RepairTemplate_NIPBL_mEGFP_mAID

Plasmid

#217664

Purpose

Repair template to endogenously tag NIPBL with mEGFP-mAID Tag

Depositor

Daniel Gerlich

Article

Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

Insert

NIPBL_mEGFP_mAID (NIPBL Human)

Tags

mAID and meGFP

Expression

Bacterial and Mammalian

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD_mCherry

Plasmid

#187387

Purpose

arabinose-inducible expression of mCherry

Depositor

Roi Avraham

Insert

mCherry

Expression

Bacterial

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEND-446_Cacnes_ProA

Plasmid

#225609

Purpose

Suicide C. acnes vector to generate ProA (PPA_RS04250) deletion leaving an Ery resistance cassette flanked by BxbI recombination sites

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pEND-224_Cacnes_sfGFP

Plasmid

#225611

Purpose

Replicative vector for green fluorescent reporter protein sfGFP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+sfGFP.

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Insert

sfGFP

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for Cutibacterium acnes

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pTlpA_mCherry_Txe/Axe

Plasmid

#229147

Purpose

Toxin-Antitoxin (Txe/Axe)

Depositor

Shrikrishnan Sankaran

Article

Dey et al Microb Biotechnol. 2023 Jun;16(6):1264-1

Insert

mCherry, Txe/Axe

Expression

Bacterial

Promoter

PtlpA (mCherry), Txe/Axe (Native)

Available Since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-GST-2

Plasmid

#228984

Purpose

For bacterial expression of anti-GST nanobody GST-2, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-2

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-1--LaG94-14

Plasmid

#228973

Purpose

For bacterial expression of anti-GFP nanobody dimer of LaG94-1 and LaG94-14, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-1--LaG94-14

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-GST-1

Plasmid

#228983

Purpose

For bacterial expression of anti-GST nanobody GST-1, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

GST-1

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaTdT-1

Plasmid

#228974

Purpose

For bacterial expression of anti-tdTomato nanobody LaTdT-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaTdT-1

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaRIgG-7

Plasmid

#228997

Purpose

For bacterial expression of anti-rabbit IgG nanobody LaRIgG-7, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaRIgG-7

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaRIgG-1

Plasmid

#228995

Purpose

For bacterial expression of anti-rabbit IgG nanobody LaRIgG-1, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaRIgG-1

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaMIgG-5

Plasmid

#228991

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-5, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-5

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHH0103_NR3C2_Hormone-recep

Plasmid

#109848

Purpose

Protein expression and purification of NR3C2_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

NR3C2_Hormone-recep (NR3C2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
SUMO-KTag

Plasmid

#51723

Purpose

Plasmid for recombinant expression in bacteria of SUMO domain fused to KTag, permitting covalent linkage to SpyTag

Depositor

Mark Howarth

Article

Fierer et al Proc Natl Acad Sci U S A. 2014 Mar 17.

Insert

SUMO-KTag

Tags

His6 Tag

Expression

Bacterial

Mutation

none

Promoter

T7

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only
pTXB1-Tn5-F

Plasmid

#160081

Purpose

Bacterial expression of C-terminally FLAG-tagged Tn5 transposase

Depositor

Pier Pandolfi

Article

Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi

Insert

Tn5 transposase

Tags

FLAG and Mxe intein - Chitin-binding domain

Expression

Bacterial

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge