30,875 results

1. GMFG

   Plasmid

   #39037

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   GMFG (GMFG Human)
   

   Tags

   His Tev

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pQE60-Exp5

   Plasmid

   #12553

   Depositor

   Ian Macara

   Article

   Brownawell et al J Cell Biol. 2002 Jan 7. 156(1):53-64.

   Insert

   EXPORTIN-5 (XPO5 Human)
   

   Tags

   HIS

   Expression

   Bacterial

   Mutation

   NONE

   Available Since

   Oct. 2, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pMRE-Tn5-153

   Plasmid

   #118535

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sYFP2
   

   Use

   Minitn5 delivery vector

   Expression

   Bacterial

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His

   Plasmid

   #171587

   Purpose

   Bacterial expression of NLS-RfxCas13d-NLS 6His

   Depositor

   Neville Sanjana

   Article

   Mendez-Mancilla et al Cell Chem Biol. 2022 Feb 17;29(2):321-32

   Insert

   MKIEE-NLS-RfxCas13d-NLS-HA-6His
   

   Tags

   HA-6HIS and MKIEE

   Expression

   Bacterial

   Available Since

   Oct. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET303-ColE1-T-E192C

   Plasmid

   #180233

   Purpose

   Expresses T domain of Colicin E1 (residues 1-190) with C terminal cysteine for maleimide chemistry

   Depositor

   Joanna Slusky

   Article

   Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

   Insert

   Colicin E1
   

   Tags

   6x His-tag

   Expression

   Bacterial

   Mutation

   Truncation of ColE1 containing T domains (residue…

   Promoter

   T7

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEND-193_Cacnes_HS

   Plasmid

   #225622

   Purpose

   C. acnes with heat shock inducible expression of pFAST. pBRESP36A with P(PPA_RS10245)+RBS_1+pFAST // P(PPA_RS06745)+RBS_1+HspR(PPA_RS10230)

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   HspR
   

   Use

   Synthetic Biology

   Tags

   pFAST-6XHis

   Expression

   Bacterial

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEND-229_Cacnes_EforCP

   Plasmid

   #225615

   Purpose

   Replicative vector for red fluorescent reporter protein eForCP strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+eForCP

   Depositor

   Marc Güell

   Article

   Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

   Insert

   EforCP
   

   Use

   Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Available Since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. BPTI-[5,55]Gly14Ile38

   Plasmid

   #195059

   Purpose

   Thermal stability of BPTI

   Depositor

   Yutaka Kuroda

   Article

   Islam et al FEBS J. 2019 Oct;286(20):4122-4134. doi:

   Insert

   bovine pancreatic trypsin inhibitor
   

   Tags

   His tag and trpΔLE leader sequences

   Expression

   Bacterial

   Mutation

   Changed Alanine residues at position of 14 and 38…

   Promoter

   T7 promotor

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSR:MSMEG_0835:Dendra

   Plasmid

   #174923

   Purpose

   C-term fluorescent fusion

   Depositor

   Keith Derbyshire, Joseph Wade

   Article

   Judd et al mBio. 2021 Mar 2;12(2). pii: mBio.02401-

   Insert

   MSMEG_0835
   

   Use

   Integrating mycobacterial expression vector attl5…

   Tags

   Gly-Ala Linker/Dendra2/1x Flag Tag

   Expression

   Bacterial

   Promoter

   pmycTetO

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-21a_2xNLS_LbCpf1_protein_expression

    Plasmid

    #114366

    Purpose

    Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

    Depositor

    Scot Wolfe

    Article

    Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

    Insert

    2xNLS_LbCpf1
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUC-UAS1B8-TEF(272)

    Plasmid

    #44376

    Depositor

    Hal Alper

    Article

    Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

    Insert

    UAS1B8-TEF(272) promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    UAS1B8-TEF(272)

    Available Since

    April 16, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pNG1256

    Plasmid

    #149710

    Purpose

    Expresses RpoA, RpoB, RpoC-9His and RpoZ from Bacillus subtilis to produce core RNAP complex

    Depositor

    Peter Lewis

    Article

    Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

    Insert

    rpoZ (rpoZ )
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCOLA-T7-YhjH

    Plasmid

    #79165

    Purpose

    Expresses YhjH under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    yhjH
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. R619-X67-527: His6-SARS-CoV-2 N

    Plasmid

    #170204

    Purpose

    E. coli expression of SARS-CoV-2 nucleocapsid protein

    Depositor

    Dominic Esposito

    Insert

    SARS-CoV-2 N (N SARS-CoV-2)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. k3(long) luciferase

    Plasmid

    #11110

    Depositor

    Anjana Rao

    Article

    Tsai et al Mol Cell Biol. 1996 Feb . 16(2):459-67.

    Insert

    k3(L) (TNF Human)
    

    Tags

    luciferase

    Expression

    Bacterial

    Available Since

    April 24, 2006

    Availability

    Academic Institutions and Nonprofits only

16. hnRNPA2_RRM1

    Plasmid

    #157865

    Purpose

    express His tagged hnRNPA2 RRM1

    Depositor

    Nicolas Fawzi

    Article

    Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

    Insert

    hnRNPA2 (HNRNPA2B1 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGLO-GFP-3UAG

    Plasmid

    #82501

    Purpose

    Expresses GFP with 3 UAG codons at the amino acid position 3, 151, and 153

    Depositor

    Chang Liu

    Article

    Schwessinger et al Integr Biol (Camb). 2016 Apr 18;8(4):542

    Insert

    Green Fluorescence Protein with 3 UAG codons
    

    Expression

    Bacterial

    Mutation

    Added an UAG codon at the amino acid position 3, …

    Promoter

    AraBAD

    Available Since

    Sept. 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. BBa_J72113-BBa_J72152

    Plasmid

    #40784

    Depositor

    Christopher Anderson

    Article

    Kittleson et al ACS Synth Biol. 2012 Dec 21;1(12):583-9.

    Insert

    Phagemid + GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. KEAP1 (KEAP1A-c029)

    Plasmid

    #110247

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    KEAP1 (KEAP1 Human)
    

    Tags

    His-TEV

    Expression

    Bacterial

    Promoter

    T7 - lacO

    Available Since

    June 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJN105-Hygro-GFP

    Plasmid

    #41883

    Depositor

    Howard Steinman

    Article

    Bandyopadhyay et al Infect Immun. 2004 Aug;72(8):4541-51.

    Insert

    Ptac::GFP-mut-2
    

    Use

    Bacterial broad host range vector

    Expression

    Bacterial

    Mutation

    GFP-mut-2 is GFP with Ser65Ala, Val68Leu, and Ser…

    Promoter

    Ptac

    Available Since

    June 11, 2013

    Availability

    Academic Institutions and Nonprofits only