32,120 results
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Plasmid#79577PurposeTP901 attB-attP. GFP will express if sites recombine.DepositorInsertGFPmut3b
ExpressionBacterialAvailable SinceJuly 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNR239
Plasmid#79576PurposeA118 attB-attP. GFP will express if sites recombine.DepositorInsertGFPmut3b
ExpressionBacterialAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pATZ-HA-GFP11
Plasmid#177720PurposeER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to an HA tag and the eleventh β-strand of GFPDepositorInsertalpha-1-antitrypsin (SERPINA1 Human)
TagsGFP11 and HAExpressionBacterial and MammalianMutationE342K (ATZ mutant)Available SinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET His6 ProteinG TEV LIC cloning vector (2P-T)
Plasmid#29713DepositorTypeEmpty backboneTagsHis6-ProteinG-TEVExpressionBacterialAvailable SinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
DDX20A
Plasmid#42376PurposeBacterial expression for structure determination; may not be full ORFDepositorAvailable SinceMarch 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
4R3H
Plasmid#64652PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertYTHDC1:JMC092-F09:C231531 (YTHDC1 Human)
Tags6xHisExpressionBacterialMutationSee commentsPromoterT7Available SinceApril 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPbcsx28
Plasmid#89909PurposeExpresses csx28 from P. buccae ATCC 33574 in bacteria. Expression driven from the Lac promoterDepositorInsertcsx28
ExpressionBacterialPromoterLacAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXW117As(RS-E11-RinA)2
Plasmid#123146PurposeArsenic sensor with three-layered amplifier cascade. Need to work with plasmid pXWPrinA-gfp2. pSB4A3 carrying J117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-tDepositorInsertJ117-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-31ECF11(ASV)-t-Pecf11-33rinA-t
UseSynthetic BiologyExpressionBacterialPromoterJ117, ParsR, PhrpLE, Pecf11Available SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXWPrinA-gfp2
Plasmid#123147PurposepSB1K3 carrying PrinA-30gfp-tDepositorInsertPrinA-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterPrinAAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET26b_Nb.AD101
Plasmid#131402PurposeE. coli periplasmic expression of conformational nanobody Nb.AD101 recognizing purified active-state A2a adenosine receptorDepositorInsertNanobody AD101
Tags6xHis-tag and pelB leaderExpressionBacterialPromoterT7Available SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pR_Lux
Plasmid#226457Purposelambda immunity region to the luciferase operon (lux)DepositorInsertlambda immunity region to the luciferase operon (lux) from Photorhabdus luminescens
ExpressionBacterialAvailable SinceNov. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII
Plasmid#174424PurposeExpresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteriaDepositorInsertDrp1
Tags6xHis(Thrombin)EGFP(TEV) and StrepIIExpressionBacterialMutationNonePromoterT7Available SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCJ202
Plasmid#162675PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.DepositorInsertPETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)
TagsHisExpressionBacterialMutationW159C, S238C; codon optimized for expression in E…PromoterT7/lacAvailable SinceFeb. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEM mouse islet1 [TJ#28]
Plasmid#16270DepositorInsertislet1 (Isl1 Mouse)
ExpressionBacterialAvailable SinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pATmin-GG
Plasmid#191352PurposeClostridium expression vector (pAMB1 origin, ermR) with drop-out Plac-RFP cassette flanked with BsaI sitesDepositorInsertPlac-RFP (IPTG-inducible red fluorescent protein)
ExpressionBacterialPromoterPlacAvailable SinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pETM41_KPNA4_Arm
Plasmid#178478PurposeBacterial expression of human domain with His-tag and MBP tagDepositorAvailable SinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHYSTC299
Plasmid#124354PurposePlasmid expressing gp41-1 intein inserted into CcdA antitoxinDepositorInsertgp41-1 intein
TagsCcdA-C (SFAD)ExpressionBacterialPromoterSTCAvailable SinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET24a MDH_9CREN
Plasmid#204243PurposeBacterial expression of I. islandicus malate dehydrogenase; Use in CURES (integration of research into undergraduate teaching labs).InsertMDH_9CREN
Tags6X HisExpressionBacterialPromoterLacAvailable SinceNov. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tus pGST-//
Plasmid#53305PurposeExpresses GST-tagged E.coli replication terminator protein Tus.DepositorInsertE.Coli Tus replication terminator protein
TagsGSTExpressionBacterialAvailable SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJL1 Aquamarine
Plasmid#106285PurposeExpresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoterDepositorInsertAquamarine
Tags6xHis and TEV cleavage (N terminal on insert)ExpressionBacterialMutationT65S and H148GPromoterT7Available SinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only