30,875 results

1. pET21-pelB-LaG-10

   Plasmid

   #172743

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-10; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-10
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pETM11-6xHis-TEV-hORF1p_K3AK4A

   Plasmid

   #202585

   Purpose

   Allows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scar

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   ORF1p
   

   Tags

   6x His and TEV protease cleavage site

   Expression

   Bacterial

   Mutation

   Changed ORF1p Lysine 3 to Alanine, changed ORF1p …

   Available Since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pALlR1

   Plasmid

   #172566

   Purpose

   Inducible gene expression, pACYC origin, LacI repressor, PLlacO-1 promoter, mRFP, Chloramphenicol Resistance Marker.

   Depositor

   Christopher Reisch

   Insert

   LacI-mRFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PLlacO-1

   Available Since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Gfp-rt-vasa

   Plasmid

   #167322

   Purpose

   Plasmid for synthesis of GFP chimeric RNA that contained vasa-3'UTRs of rainbow trout by in vitro transcription.

   Depositor

   Goro Yoshizaki

   Article

   Yoshizaki et al Biol Reprod. 2005 Jul;73(1):88-93. doi:

   Insert

   rt vasa 3'UTRs (ddx4 rainbow trout)
   

   Expression

   Bacterial

   Available Since

   June 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. ptRNA100

   Plasmid

   #154133

   Purpose

   Expresses a set of tRNAs in bacterial cells

   Depositor

   Alexander Mankin

   Article

   Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

   Insert

   tRNAs operon under control of Ptac promoter
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBSV2_Psyn-sgRNA500

   Plasmid

   #149613

   Purpose

   gRNA expression in B. burgdorferi, parental vector

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   Psyn

   Available Since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. 6xHis-TEV-ATG3 (Human autophagy E2-like enzyme)

   Plasmid

   #169079

   Purpose

   Human ATG3

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Insert

   ATG3 (ATG3 Human)
   

   Tags

   6X Histidine Tag, TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 lac promoter

   Available Since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET303-colE1TR

   Plasmid

   #155181

   Purpose

   Expresses colicin E1 residues 1-364

   Depositor

   Joanna Slusky

   Article

   Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

   Insert

   colicin E1 residues 1-364
   

   Tags

   C-terminal 6x Histidine tag

   Expression

   Bacterial

   Mutation

   truncation of colicin E1 including 1-364

   Promoter

   T7

   Available Since

   Aug. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. MDA5A

   Plasmid

   #42394

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MDA5A (IFIH1 Human)
   

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   See Comments

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

10. B3-2

    Plasmid

    #111472

    Purpose

    translational elongation factor 4

    Depositor

    Cheemeng Tan

    Article

    Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

    Insert

    translational elongation factor 4
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAbaMCi-GFP-gfp_pJMP2768

    Plasmid

    #208889

    Purpose

    Acinetobacter baumannii CRISPRi test plasmid (GFP, gfp-targeting gRNA).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette, sfGFP
    

    Expression

    Bacterial

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRT7 NT Topo arabinose

    Plasmid

    #73446

    Purpose

    Used for arabinose-induced expression of N-term GST/C-term 6xHis tagged peptides

    Depositor

    Jesse Rinehart

    Type

    Empty backbone

    Tags

    GST (N-terminal), 6xHis (C-terminal)

    Expression

    Bacterial

    Promoter

    pBad

    Available Since

    March 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMBP-HIS2-Vps2

    Plasmid

    #21494

    Depositor

    James Hurley

    Article

    Wollert et al Nature. 2009 Mar 12. 458(7235):172-7.

    Insert

    VPS2 (DID4 Budding Yeast)
    

    Tags

    6x His and MBP

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Sept. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pSUMO-YHRC

    Plasmid

    #54336

    Purpose

    Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.

    Depositor

    Claes Andréasson

    Article

    Holmberg et al Protein Expr Purif. 2014 Jun;98:38-45. d

    Type

    Empty backbone

    Tags

    6His-SUMO (yeast gene: SMT3)

    Expression

    Bacterial

    Promoter

    T7-LacO

    Available Since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYTK047

    Plasmid

    #65154

    Purpose

    Encodes GFP dropout as a Type 234r part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    GFP dropout
    

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pfMDH_WT

    Plasmid

    #67721

    Purpose

    Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Article

    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

    Insert

    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    

    Tags

    6x-His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

17. p38delta

    Plasmid

    #29575

    Depositor

    Dustin Maly

    Article

    Hari et al Chem Biol. 2013 Jun 20;20(6):806-15. doi

    Insert

    p38delta (Mapk13 Mouse)
    

    Tags

    HIS6 and TEV

    Expression

    Bacterial

    Mutation

    Starting Met replaced with Ala

    Available Since

    July 13, 2011

    Availability

    Academic Institutions and Nonprofits only

18. pHH0103_RBM17_RRM

    Plasmid

    #109786

    Purpose

    Protein expression and purification of RBM17_RRM

    Depositor

    Sachdev Sidhu

    Article

    Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

    Insert

    RBM17_RRM (RBM17 Human)
    

    Expression

    Bacterial

    Available Since

    Oct. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJUMP44-2A(sfGFP)

    Plasmid

    #126988

    Purpose

    Level 2 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

    Depositor

    Chris French

    Article

    Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    None

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCOLA-T7-WspR:D70E

    Plasmid

    #79164

    Purpose

    Expresses constitutively active WspR D70E under a T7 promoter

    Depositor

    Ming Hammond

    Article

    Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

    Insert

    WspR (wspR P. areuginosa)
    

    Expression

    Bacterial

    Mutation

    D70E

    Promoter

    T7

    Available Since

    Aug. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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