31,059 results
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Plasmid#199754PurposeExpresses codon optimized thermostabile TccCas13a in E.coliDepositorInsertTccCas13a
TagsSUMOExpressionBacterialPromoterLacIAvailable SinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS413-GAL1-yEGFP1
Plasmid#40235DepositorInsertyEGFP1
TagsHIS3MX6ExpressionBacterialPromoterGAL1Available SinceOct. 25, 2012AvailabilityAcademic Institutions and Nonprofits only -
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Ydf NanoLuc
Plasmid#225656PurposeReporter plasmid expressing NanoLuciferase under the control of the promoter PydfJ115, cognate to YdfI. Responds to NarL/NarX chimeras from this study.DepositorInsertNanoLuciferase
UseCell free expressionTagsMycExpressionBacterialPromoterPydfJ115Available SinceDec. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarL YdfI Flag
Plasmid#225650PurposeExpresses NarL with a mutated DNA binding domain (YdfI). For use with NarX chimeras and reporter plasmids from this study.DepositorInsertNarL-YdfI (narL Synthetic)
UseCell free expressionTagsFLAGExpressionBacterialMutationNarL with native DNA-binding domain replaced with…PromoterT7Available SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL NarX Myc
Plasmid#225651PurposeExpresses WT NarX capable of sensing nitrate. For use with NarL-YdfI and reporter plasmids from this study.DepositorAvailable SinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-1
Plasmid#163127PurposepLyGo cloning vector for cytoplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding Ubiquitin with an internal His10-tag and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyTagsUbiquitinExpressionBacterialPromoterT7Available SinceMay 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp5_Mpro
Plasmid#156475PurposeBacterial expression of Sars-CoV2 Nsp5_Mpro protein with His-tag and GST-tagDepositorInsertNsp5_Mpro (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpyC-GFP
Plasmid#220191PurposesgRNA expression vector - pBG42 promoter with GFP in sgRNA siteDepositorInsertpBG42 promoter with GFP in sgRNA site
UseCRISPRExpressionBacterialMutationmonomeric superfolder green fluorescent proteinPromoterpBG42Available SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRS-LacI-dCas9
Plasmid#220194Purposemini-Tn7 dCas9 expression vector - LacI/pLac promoterDepositorInsertPseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
UseCRISPRExpressionBacterialMutationPseudomonas aeruginosa codon optimizedPromoterLacI/pLacAvailable SinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only