30,875 results

1. pET22b-ecDHFR

   Plasmid

   #109055

   Purpose

   Bacterial expression of E.coli DHFR protein

   Depositor

   Stephen Benkovic

   Article

   Cameron et al Biochemistry. 1997 Dec 16;36(50):15792-8

   Insert

   ecDHFR
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28_6xHis-PARP1delWGR

   Plasmid

   #173940

   Purpose

   Bacterial expression of PARP1 lacking WGR domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delWGR (PARP1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 521-660; V762A

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. IVT-MCP-MMLV-RT

   Plasmid

   #207457

   Purpose

   in vitro transcription of mRNA

   Depositor

   Erik Sontheimer

   Article

   Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

   Insert

   MCP-RT
   

   Expression

   Bacterial

   Available Since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pVRa20_992

   Plasmid

   #49673

   Depositor

   Christopher Voigt

   Article

   Rhodius et al Mol Syst Biol. 2013 Oct 29;9:702. doi: 1

   Insert

   ECF20_992
   

   Use

   Synthetic Biology

   Tags

   His6-PreScission

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pT7-lacO

   Available Since

   Jan. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. CBX7

   Plasmid

   #25241

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CBX7_04 (CBX7 Human)
   

   Tags

   6x His and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 13, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

6. pOT2

   Plasmid

   #14460

   Depositor

   Philip Poole

   Article

   Karunakaran et al Microbiology. 2005 Oct . 151(Pt 10):3249

   Insert

   Gfp-UV
   

   Expression

   Bacterial

   Available Since

   April 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGBW-m4046955

   Plasmid

   #145616

   Purpose

   Bacterial Expression plasmid for RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   RNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;FLAG_2

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pCOLA-Spec-yibDp-Erv1p

   Plasmid

   #212094

   Purpose

   Expresses sulfydryl oxidase (Erv1p) after phosphate depletion

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Insert

   Sulfydryl oxidase (ERV1 Budding Yeast)
   

   Expression

   Bacterial

   Promoter

   yibDp

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCOLA-Spec-EV

   Plasmid

   #212095

   Purpose

   (Empty Backbone) pCOLA-Spec Empty Vector

   Depositor

   Michael Lynch

   Article

   Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

   Type

   Empty backbone

   Expression

   Bacterial

   Available Since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHHYTK113

    Plasmid

    #200144

    Purpose

    Type 4a part for Modular Cloning in Yeast

    Depositor

    Johannes Herrmann

    Article

    Simakin et al Microb Cell. 2023 Feb 28;10(4):78-87. do

    Insert

    sfGFP1-10
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pITC

    Plasmid

    #192856

    Purpose

    Cloning and expression of transcription factor gene under control of naringenin inducible promoter

    Depositor

    Eveline Peeters

    Article

    Bernauw et al Methods Mol Biol. 2022;2516:113-141. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    PfdeAR

    Available Since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFE-Rs-FI-prk

    Plasmid

    #162697

    Purpose

    Expresses R. spaeroides Form IC rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    R. spaeroides Form IC rubisco and S. elongatus Prk
    

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Promoter

    PLtet0-1 promoter

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCB’ CbbL Y72R

    Plasmid

    #162707

    Purpose

    Carboxysome operon with point mutation to CbbL, prk; Substitution of CsoS2-binding tyrosine with arginine prevents rubisco encapsulation creating rubisco-less carboxysomes

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    pHnCB10 derived carboxysome operon, prk
    

    Expression

    Bacterial

    Mutation

    CbbL Y72R; Substitution of CsoS2-binding tyrosine…

    Promoter

    PLtet0-1 promoter

    Available Since

    March 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFE-Rrub-FII-prk

    Plasmid

    #162695

    Purpose

    Expresses R. rubrum Form II rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Inserts

    Rubrum Form II rubisco
    S. elongatus PRK
    

    Expression

    Bacterial

    Promoter

    PLtet0-1 promoter and under same promoter

    Available Since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYTK048

    Plasmid

    #65155

    Purpose

    Encodes Spacer as a Type 234 part to be used in the Dueber YTK system

    Depositor

    John Dueber

    Article

    Lee et al ACS Synth Biol. 2015 Apr 14.

    Insert

    Spacer
    

    Expression

    Bacterial

    Available Since

    June 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGBW-m4046760

    Plasmid

    #145600

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pOPINVHH_his

    Plasmid

    #210405

    Purpose

    For expression of VHH with C-terminal his tag

    Depositor

    Ray Owens

    Article

    Eyssen et al Bio Protoc. 2024 Mar 20;14(6):e4962. doi

    Type

    Empty backbone

    Tags

    his6

    Expression

    Bacterial

    Promoter

    lac

    Available Since

    March 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAcCAST-sgRNA_entry (CJT83)

    Plasmid

    #181785

    Purpose

    Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AcTnsB, AcTnsC, AcTniQ, AcCas12k
    

    Expression

    Bacterial

    Promoter

    Lac and J23119

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCoofy18

    Plasmid

    #43975

    Depositor

    Sabine Suppmann

    Article

    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.

    Type

    Empty backbone

    Tags

    His10

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCri_L706-antiSH2-OptoMB

    Plasmid

    #159935

    Purpose

    Opto-MB bacterial expression

    Depositor

    Jose Avalos, Jared Toettcher

    Article

    Carrasco-Lopez et al Nat Commun. 2020 Aug 13;11(1):4045. doi:

    Insert

    anti-SH2 MB with SS58 insert
    

    Expression

    Bacterial

    Available Since

    Oct. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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